SEC6_ARATH
ID SEC6_ARATH Reviewed; 752 AA.
AC Q94AI6; Q8RVQ6; Q9M9G4;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Exocyst complex component SEC6;
DE Short=AtSec6;
GN Name=SEC6; OrderedLocusNames=At1g71820; ORFNames=F14O23.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Elias M., Cvrckova F., Zarsky V.;
RT "Molecular characterization of the exocyst complex in Arabidopsis
RT thaliana.";
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP COMPONENT OF THE EXOCYST COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=18492870; DOI=10.1105/tpc.108.059105;
RA Hala M., Cole R., Synek L., Drdova E., Pecenkova T., Nordheim A.,
RA Lamkemeyer T., Madlung J., Hochholdinger F., Fowler J.E., Zarsky V.;
RT "An exocyst complex functions in plant cell growth in Arabidopsis and
RT tobacco.";
RL Plant Cell 20:1330-1345(2008).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=19895414; DOI=10.1111/j.1469-8137.2009.03070.x;
RA Chong Y.T., Gidda S.K., Sanford C., Parkinson J., Mullen R.T., Goring D.R.;
RT "Characterization of the Arabidopsis thaliana exocyst complex gene families
RT by phylogenetic, expression profiling, and subcellular localization
RT studies.";
RL New Phytol. 185:401-419(2010).
RN [7]
RP FUNCTION, INTERACTION WITH EXO84B, AND SUBCELLULAR LOCATION.
RX PubMed=20870962; DOI=10.1105/tpc.110.074351;
RA Fendrych M., Synek L., Pecenkova T., Toupalova H., Cole R., Drdova E.,
RA Nebesarova J., Sedinova M., Hala M., Fowler J.E., Zarsky V.;
RT "The Arabidopsis exocyst complex is involved in cytokinesis and cell plate
RT maturation.";
RL Plant Cell 22:3053-3065(2010).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23283982; DOI=10.1091/mbc.e12-06-0492;
RA Fendrych M., Synek L., Pecenkova T., Drdova E.J., Sekeres J., de Rycke R.,
RA Nowack M.K., Zarsky V.;
RT "Visualization of the exocyst complex dynamics at the plasma membrane of
RT Arabidopsis thaliana.";
RL Mol. Biol. Cell 24:510-520(2013).
RN [10]
RP FUNCTION, INTERACTION WITH KEU, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23702595; DOI=10.1093/mp/sst082;
RA Wu J., Tan X., Wu C., Cao K., Li Y., Bao Y.;
RT "Regulation of cytokinesis by exocyst subunit SEC6 and KEULE in Arabidopsis
RT thaliana.";
RL Mol. Plant 6:1863-1876(2013).
RN [11]
RP INTERACTION WITH EXO70E2, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24307681; DOI=10.1091/mbc.e13-10-0586;
RA Ding Y., Wang J., Chun Lai J.H., Ling Chan V.H., Wang X., Cai Y., Tan X.,
RA Bao Y., Xia J., Robinson D.G., Jiang L.;
RT "Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in
RT both plants and animals.";
RL Mol. Biol. Cell 25:412-426(2014).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane during
CC regulated or polarized secretion. Involved in polarized cell growth and
CC organ morphogenesis. During cytokinesis, involved in cell plate
CC initiation, cell plate maturation and formation of new primary cell
CC wall. {ECO:0000269|PubMed:20870962, ECO:0000269|PubMed:23283982,
CC ECO:0000269|PubMed:23702595}.
CC -!- SUBUNIT: The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8,
CC SEC10, EXO70A1 and EXO84B. Interacts with EXO84B and KEU (via C-
CC terminus). Binds to EXO70E2 (PubMed:24307681).
CC {ECO:0000269|PubMed:20870962, ECO:0000269|PubMed:23702595,
CC ECO:0000269|PubMed:24307681}.
CC -!- INTERACTION:
CC Q94AI6; Q9LZD3: EXO70A1; NbExp=3; IntAct=EBI-1797182, EBI-1797218;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19895414,
CC ECO:0000269|PubMed:23283982, ECO:0000269|PubMed:24307681}. Cell
CC membrane {ECO:0000269|PubMed:23283982}. Cytoplasm, cytoskeleton,
CC phragmoplast {ECO:0000269|PubMed:20870962,
CC ECO:0000269|PubMed:23702595}. Secreted, cell wall
CC {ECO:0000269|PubMed:20870962}. Secreted, extracellular exosome
CC {ECO:0000269|PubMed:24307681}. Note=During cytokinesis, localizes to
CC the nascent cell plate and later to the cell plate insertion site and
CC along the post-cytokinetic wall (PubMed:20870962). Shuttles from the
CC cytoplasm to the exocyst-positive organelle (EXPO) in the presence of
CC EXO70E2 (PubMed:24307681). {ECO:0000269|PubMed:20870962,
CC ECO:0000269|PubMed:24307681}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q94AI6-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Male gametophytic lethal due to defect in pollen
CC germination and pollen tube growth. {ECO:0000269|PubMed:18492870,
CC ECO:0000269|PubMed:23702595}.
CC -!- SIMILARITY: Belongs to the SEC6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF43235.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAL87122.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF479279; AAL87122.1; ALT_INIT; mRNA.
DR EMBL; AC012654; AAF43235.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35236.1; -; Genomic_DNA.
DR EMBL; AY046014; AAK76688.1; -; mRNA.
DR EMBL; AY133819; AAM91753.1; -; mRNA.
DR PIR; G96740; G96740.
DR RefSeq; NP_565026.1; NM_105840.4. [Q94AI6-1]
DR AlphaFoldDB; Q94AI6; -.
DR SMR; Q94AI6; -.
DR BioGRID; 28732; 7.
DR IntAct; Q94AI6; 3.
DR STRING; 3702.AT1G71820.2; -.
DR TCDB; 1.F.2.1.3; the octameric exocyst (exocyst) family.
DR iPTMnet; Q94AI6; -.
DR PRIDE; Q94AI6; -.
DR ProteomicsDB; 232782; -. [Q94AI6-1]
DR EnsemblPlants; AT1G71820.1; AT1G71820.1; AT1G71820. [Q94AI6-1]
DR GeneID; 843512; -.
DR Gramene; AT1G71820.1; AT1G71820.1; AT1G71820. [Q94AI6-1]
DR KEGG; ath:AT1G71820; -.
DR Araport; AT1G71820; -.
DR eggNOG; KOG2286; Eukaryota.
DR HOGENOM; CLU_011776_1_0_1; -.
DR OMA; MNIGPKT; -.
DR PhylomeDB; Q94AI6; -.
DR PRO; PR:Q94AI6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94AI6; baseline and differential.
DR Genevisible; Q94AI6; AT.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0051601; P:exocyst localization; IBA:GO_Central.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR Gene3D; 1.10.357.70; -; 1.
DR InterPro; IPR010326; EXOC3/Sec6.
DR InterPro; IPR042532; EXOC3/Sec6_C.
DR PANTHER; PTHR21292; PTHR21292; 1.
DR Pfam; PF06046; Sec6; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell membrane; Cell wall; Cytoplasm;
KW Cytoskeleton; Exocytosis; Membrane; Reference proteome; Secreted;
KW Transport.
FT CHAIN 1..752
FT /note="Exocyst complex component SEC6"
FT /id="PRO_0000424571"
FT REGION 229..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 752 AA; 85674 MW; 4A775EB27FC24CFA CRC64;
MMVEDLGVEA KEAAVREVAK LLPLPELLQS ISSIKADYIA RQQANDAQLS TMVAEQVEQA
QAGLESLSSS EKTIYELRDN FISIDKLCQE CQTLIDNHDQ IKLLSNARNN LNKTLKDVEG
MMSISVEAAA ARDSLSDDKE IVNTYERLTA LDGKRRFALA AAGEEVGRLR EYFEDVDRTW
ETFEKTLWGH VSNYYKLSKE SPQTLVRALR VVEMQEILDQ QLAEEAAEAE GEGAMASVAN
PRRPGKKSTT TSASSKGLAQ QKLKVQGKGY KDKCYEQIRK AVEDRFNRLL TLVFEDLKAA
LEEARMIGEE LGDIYDYVAP CFPPRYEIFQ LMVNLYTERF IQMLRLLSDR ANDLTNIEIL
KVTGWVVEYQ ENLIALGVDD SLAQVCSESG SMDPLMNAYV ERMQATTKKW YMNILEADKV
QPPKKTEEGK LYTPAAVDLF RILGEQVQIV RDNSTDVMLY RIALAIIQVM IDFQAAEKKR
VDEPASDIGL EPLCAMINNN LRCYDLAMEL SNSTLEALPQ NYAEQVNFED TCKGFLEVAK
EAVHQTVRVI FEDPGVQELL VKLYQKEWCE GQVTEYLVAT FGDYFTDVKM YVEERSFRRF
VEACLEETVV VYVDHLLTQK NYIKEETIER MRLDEEVLMD FFREYISASK VESRIRIMSD
LRELASAESL DAFTLVYSNI LEHQPDCPAE VVEKLVSLRE GIPRKDTKEV VQECKEIYEN
TLVDGNPPKT GFVFPRVKCL TASKGSMWRK LT
