SEC74_SCHPO
ID SEC74_SCHPO Reviewed; 928 AA.
AC Q10491; Q9URW4; Q9UU74;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Arf guanine nucleotide exchange factor sec74;
GN Name=sec74; ORFNames=SPAC26F1.01, SPAPJ691.01c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 53-207.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Guanine nucleotide exchange factor for Arf GTPases,
CC stimulating the nucleotide exchange from the GDP-bound to the GTP-bound
CC form. Involved in vesicular transport (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Cell
CC septum {ECO:0000269|PubMed:16823372}. Cell tip
CC {ECO:0000269|PubMed:16823372}.
CC -!- DOMAIN: The SEC7 domain is sufficient for nucleotide exchange activity.
CC {ECO:0000250}.
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DR EMBL; CU329670; CAA97358.1; -; Genomic_DNA.
DR EMBL; AB027773; BAA87077.1; -; Genomic_DNA.
DR PIR; T38419; T38419.
DR RefSeq; NP_594894.2; NM_001020323.2.
DR AlphaFoldDB; Q10491; -.
DR SMR; Q10491; -.
DR BioGRID; 278550; 17.
DR STRING; 4896.SPAC26F1.01.1; -.
DR iPTMnet; Q10491; -.
DR MaxQB; Q10491; -.
DR PaxDb; Q10491; -.
DR PRIDE; Q10491; -.
DR EnsemblFungi; SPAC26F1.01.1; SPAC26F1.01.1:pep; SPAC26F1.01.
DR GeneID; 2542073; -.
DR KEGG; spo:SPAC26F1.01; -.
DR PomBase; SPAC26F1.01; sec74.
DR VEuPathDB; FungiDB:SPAC26F1.01; -.
DR eggNOG; KOG0929; Eukaryota.
DR HOGENOM; CLU_315269_0_0_1; -.
DR InParanoid; Q10491; -.
DR OMA; SSDECYI; -.
DR PhylomeDB; Q10491; -.
DR Reactome; R-SPO-199992; trans-Golgi Network Vesicle Budding.
DR Reactome; R-SPO-5620916; VxPx cargo-targeting to cilium.
DR Reactome; R-SPO-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SPO-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-SPO-6811438; Intra-Golgi traffic.
DR PRO; PR:Q10491; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0032153; C:cell division site; HDA:PomBase.
DR GO; GO:0030428; C:cell septum; IEA:UniProtKB-SubCell.
DR GO; GO:0051286; C:cell tip; HDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:PomBase.
DR GO; GO:0008289; F:lipid binding; ISM:PomBase.
DR GO; GO:0006887; P:exocytosis; ISO:PomBase.
DR GO; GO:0006886; P:intracellular protein transport; IC:PomBase.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR GO; GO:0023052; P:signaling; NAS:PomBase.
DR Gene3D; 1.10.1000.11; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF01369; Sec7; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48425; SSF48425; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..928
FT /note="Arf guanine nucleotide exchange factor sec74"
FT /id="PRO_0000120221"
FT DOMAIN 228..420
FT /note="SEC7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00189"
FT DOMAIN 548..677
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 928 AA; 103633 MW; 6D9EA3CA411B8E80 CRC64;
MDESSRIASS SALHGLDEMV SAHKPSPPLP SRRKGKSALR SALEKKNRKS KSKPKVTITS
DTPKVSSQHS PVSSAYTGDS TTDLDSKSGH SSSQKLSNKV SSALKLTIPK RWRSSKSSSS
QCSSPFLPTS SSNGHGDDAS LNLPDKKSRP SSQSSIFLSN WSTIFSSNAS PTDSQLSPTH
TSTIAELAAS TLSIFPSGSY AGSTFGSPSR SSIDSSTYLP RSKSVNSLSS NFSARTPASN
QSSVSEDFGA APNCDHKHNS VTLSDFALPD IDQHDTVETI LEKVEFTIPK QFTPAILSQG
TSSLLKLCLR KYLSVVNFKC ISLDMALRKF LAVYVLPNET QQIDRVLSTF SDQYFHCNPD
LYDSSDECYI LTFSLMILHT DFFNIHNRQK MTRAEFISNT NLPTILPEIL ECFYDNITYT
PFVHVEHIGC VVTSPSNEKP SLSQRLGDSQ FRLTKRNTFA TESDHQALQE QLTGFRINLD
SILDLKKIDS VRTSIESPTR TADDLYKEFA RAPFLQIVSH RSQPQAFSYH FEPSAESEST
NPAIINVKVF KLGILIQNEK VRKDRLLSNR EVGVILTSSQ LMFFKNVYWI SGLLDQLEDF
KRSHSFSPCY FSPHLTSLSA DYIIPLSDLV AYGDGSRIEN ELYSFSVKQK NQRTHVFSVT
NVDELNDWLV KINFVSTFAT AGLAPRERLP CVESSDKSIK ASVSVLPDIY EDSDAMSKAS
KETRSEVLEC SKVRVYIVQN RIYKLEEALR QGESKMTVQR RNAANILHLE PIQSRTRIRL
ARCSNTLKKN MLAQMIEIQK FKISIKFLKE DLEMDSHLQD YLRSVFPSSV MGKENASEDT
ILESNFRNHS NPSVRRSNLR VGKTVESVQN TKLKSVENDS GKAETETVGK NPEVVRKSPA
KLPNIANIMT VNGHRYSVVE LPDDFLRE
