SEC8_ARATH
ID SEC8_ARATH Reviewed; 1053 AA.
AC Q93YU5; Q541W5; Q9SS49;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Exocyst complex component SEC8;
DE Short=AtSec8;
DE AltName: Full=Exocyst complex component 4;
GN Name=SEC8; OrderedLocusNames=At3g10380; ORFNames=F14P13.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16040664; DOI=10.1104/pp.105.062273;
RA Cole R.A., Synek L., Zarsky V., Fowler J.E.;
RT "SEC8, a subunit of the putative Arabidopsis exocyst complex, facilitates
RT pollen germination and competitive pollen tube growth.";
RL Plant Physiol. 138:2005-2018(2005).
RN [6]
RP COMPONENT OF THE EXOCYST COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=18492870; DOI=10.1105/tpc.108.059105;
RA Hala M., Cole R., Synek L., Drdova E., Pecenkova T., Nordheim A.,
RA Lamkemeyer T., Madlung J., Hochholdinger F., Fowler J.E., Zarsky V.;
RT "An exocyst complex functions in plant cell growth in Arabidopsis and
RT tobacco.";
RL Plant Cell 20:1330-1345(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=19895414; DOI=10.1111/j.1469-8137.2009.03070.x;
RA Chong Y.T., Gidda S.K., Sanford C., Parkinson J., Mullen R.T., Goring D.R.;
RT "Characterization of the Arabidopsis thaliana exocyst complex gene families
RT by phylogenetic, expression profiling, and subcellular localization
RT studies.";
RL New Phytol. 185:401-419(2010).
RN [9]
RP FUNCTION.
RX PubMed=20618910; DOI=10.1111/j.1469-8137.2010.03372.x;
RA Kulich I., Cole R., Drdova E., Cvrckova F., Soukup A., Fowler J.,
RA Zarsky V.;
RT "Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1
RT are involved in the localized deposition of seed coat pectin.";
RL New Phytol. 188:615-625(2010).
RN [10]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=20870962; DOI=10.1105/tpc.110.074351;
RA Fendrych M., Synek L., Pecenkova T., Toupalova H., Cole R., Drdova E.,
RA Nebesarova J., Sedinova M., Hala M., Fowler J.E., Zarsky V.;
RT "The Arabidopsis exocyst complex is involved in cytokinesis and cell plate
RT maturation.";
RL Plant Cell 22:3053-3065(2010).
RN [11]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24307681; DOI=10.1091/mbc.e13-10-0586;
RA Ding Y., Wang J., Chun Lai J.H., Ling Chan V.H., Wang X., Cai Y., Tan X.,
RA Bao Y., Xia J., Robinson D.G., Jiang L.;
RT "Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in
RT both plants and animals.";
RL Mol. Biol. Cell 25:412-426(2014).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane during
CC regulated or polarized secretion. Involved in polarized cell growth and
CC organ morphogenesis. During cytokinesis, involved in secretory
CC processes during cell plate initiation, cell plate maturation and
CC formation of new primary cell wall.
CC -!- FUNCTION: Participates in polarized pectin delivery required for the
CC polarized development of the mucilage-producing volcano cells of the
CC seed coat.
CC -!- SUBUNIT: The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8,
CC SEC10, EXO70A1 and EXO84B.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19895414,
CC ECO:0000269|PubMed:24307681}. Cytoplasm, cytoskeleton, phragmoplast
CC {ECO:0000269|PubMed:20870962}. Secreted, cell wall
CC {ECO:0000269|PubMed:20870962}. Secreted, extracellular exosome
CC {ECO:0000269|PubMed:24307681}. Note=During cytokinesis, localizes to
CC the nascent cell plate and later to the cell plate insertion site and
CC along the post-cytokinetic wall (PubMed:20870962). Shuttles from the
CC cytoplasm to the exocyst-positive organelle (EXPO) in the presence of
CC EXO70E2 (PubMed:24307681). {ECO:0000269|PubMed:20870962,
CC ECO:0000269|PubMed:24307681}.
CC -!- DISRUPTION PHENOTYPE: Male gametophytic lethal due to defect in pollen
CC germination and pollen tube growth. {ECO:0000269|PubMed:16040664,
CC ECO:0000269|PubMed:18492870}.
CC -!- SIMILARITY: Belongs to the SEC8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF02806.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009400; AAF02806.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74898.1; -; Genomic_DNA.
DR EMBL; AK118541; BAC43144.1; -; mRNA.
DR EMBL; AY059763; AAL24111.1; -; mRNA.
DR RefSeq; NP_566372.1; NM_111873.4.
DR AlphaFoldDB; Q93YU5; -.
DR BioGRID; 5535; 7.
DR IntAct; Q93YU5; 2.
DR STRING; 3702.AT3G10380.1; -.
DR TCDB; 1.F.2.1.3; the octameric exocyst (exocyst) family.
DR iPTMnet; Q93YU5; -.
DR PaxDb; Q93YU5; -.
DR PRIDE; Q93YU5; -.
DR ProteomicsDB; 232779; -.
DR EnsemblPlants; AT3G10380.1; AT3G10380.1; AT3G10380.
DR GeneID; 820201; -.
DR Gramene; AT3G10380.1; AT3G10380.1; AT3G10380.
DR KEGG; ath:AT3G10380; -.
DR Araport; AT3G10380; -.
DR TAIR; locus:2076249; AT3G10380.
DR eggNOG; ENOG502QRJ3; Eukaryota.
DR HOGENOM; CLU_009514_0_0_1; -.
DR InParanoid; Q93YU5; -.
DR OMA; SLPNWTD; -.
DR OrthoDB; 96167at2759; -.
DR PhylomeDB; Q93YU5; -.
DR PRO; PR:Q93YU5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q93YU5; baseline and differential.
DR Genevisible; Q93YU5; AT.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0000145; C:exocyst; IDA:TAIR.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0060321; P:acceptance of pollen; IMP:TAIR.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0048354; P:mucilage biosynthetic process involved in seed coat development; IMP:TAIR.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IEA:InterPro.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IEA:InterPro.
DR InterPro; IPR039682; Sec8/EXOC4.
DR InterPro; IPR007191; Sec8_exocyst_N.
DR PANTHER; PTHR14146; PTHR14146; 2.
DR Pfam; PF04048; Sec8_exocyst; 1.
PE 1: Evidence at protein level;
KW Cell wall; Coiled coil; Cytoplasm; Cytoskeleton; Exocytosis;
KW Reference proteome; Secreted; Transport.
FT CHAIN 1..1053
FT /note="Exocyst complex component SEC8"
FT /id="PRO_0000118939"
FT REGION 255..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 91..121
FT /evidence="ECO:0000255"
FT COMPBIAS 255..274
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1053 AA; 116605 MW; A8D6559FFDA0E039 CRC64;
MGIFNGLPVP SDKTYLREEL ARIDESWAAA RFDSLPHVVH ILTSKDREAD IHILKEQSDV
VEEVVDEVVH AYHGGFNKAI QNYSQILRLF SESTEKIGDL KHDLAEAKQS LGARNKQLHQ
LWYRSVTLRH IISLLDQIEG IAKVPSRIEK LIADKQFYAA IQVYLQSSLM LEREGLQTVG
ALQDVRSELT KLRGALFFKI LDDLHAHLYN RGEYSSVASS IYERDDEVPT TTAVAASRMS
SQPLSRRTRT LKGDSQFGVR GLTNGSYRTA SNDESSSFDG HDEEDSVEHD ESTADTARNG
TDSKLLSHQL PPWLSDSTPD EFIEAVRKSD DPLHVKYLQT LVQCLCMLGK VAAAGAIICQ
KLRPTIHEII ISKIKAHLET TNLSKSACSQ GDRSVAAGLH LIKGQSEAYR LSKEKPQNGI
SNSGTHLAVS PVSPLMAPGG KAQAAAKELL DSILDTIVKI FENHVVIGEL LEMKASQHDI
NTPRSLPTDV NWNTESEASQ ATGGYTISFP LTVLQSECQQ LICEILRATP EAASADAAAQ
TAKLAKKAPK KDKRDAPEDG LTFTFRFTDA TVSISNQGAD LIRQGWGKRA PNASLEGYGS
AAVLPEQGIY LAASIYRPVL QFTDKITSML PKKHSQLVND GLLTFTENFV KDHLLPTMFV
DYRKGVQQAI SSAAAFRPRA HTTTYTATVE KGRPILQGLL AIDLLAKEVL GWAQAMPKFA
TDLVKYVQTF LERTFERCRT SYMEAVLEKL SYMLIGRHDI EKLMRLDAAS ACLPSTLGHA
VSHSEAVGTE VELSDLFLSL RPIKQDNLIR DDNKLILLAS LSDSLEYVAD SIERLGQAVP
RVASQAEGNS RNQAASPRNL ASFADEYRKL ATDCLKVLRV EMQLETVFHL QEMTNREYLE
DEDAEEPDDF VISLTSQITR REEGMAPFIS GEKRNYVFGG ISGIAANASI KALADMRSIN
LFGVQQICRN TIAVEQAMAA IPYIDGETVQ QNLDRVRTYF ELLNMPFEAL LAFIAEHDQM
FTPTEYSNLL KVNVPGRDTP SDAQSRLLEI LSH
