BG4_ARATH
ID BG4_ARATH Reviewed; 345 AA.
AC O49352; Q84LG3; Q8LEU0;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable glucan endo-1,3-beta-glucosidase BG4 {ECO:0000305};
DE EC=3.2.1.39 {ECO:0000305};
DE AltName: Full=Beta-1,3-glucanase 4 {ECO:0000305};
DE Flags: Precursor;
GN Name=BG4 {ECO:0000303|PubMed:10092183};
GN Synonyms=BETAG4 {ECO:0000312|EMBL:AED92832.1};
GN OrderedLocusNames=At5g20330 {ECO:0000312|Araport:AT5G20330};
GN ORFNames=F5O24.220 {ECO:0000312|EMBL:AF296825};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10092183; DOI=10.1023/a:1006194822666;
RA Delp G., Palva E.T.;
RT "A novel flower-specific Arabidopsis gene related to both pathogen-induced
RT and developmentally regulated plant beta-1,3-glucanase genes.";
RL Plant Mol. Biol. 39:565-575(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 257-345.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: May play a role in plant defense against pathogens.
CC {ECO:0000250|UniProtKB:F4J270}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P33157}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; X79694; CAA56134.1; -; Genomic_DNA.
DR EMBL; AF296825; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92832.1; -; Genomic_DNA.
DR EMBL; AY085241; AAM62473.1; -; mRNA.
DR EMBL; BT003836; AAO41887.1; -; mRNA.
DR RefSeq; NP_197533.1; NM_122040.4.
DR AlphaFoldDB; O49352; -.
DR SMR; O49352; -.
DR STRING; 3702.AT5G20330.1; -.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR PaxDb; O49352; -.
DR PRIDE; O49352; -.
DR ProteomicsDB; 240329; -.
DR EnsemblPlants; AT5G20330.1; AT5G20330.1; AT5G20330.
DR GeneID; 832155; -.
DR Gramene; AT5G20330.1; AT5G20330.1; AT5G20330.
DR KEGG; ath:AT5G20330; -.
DR Araport; AT5G20330; -.
DR TAIR; locus:2149279; AT5G20330.
DR eggNOG; ENOG502QVKW; Eukaryota.
DR HOGENOM; CLU_024953_0_0_1; -.
DR InParanoid; O49352; -.
DR OMA; WFAANIE; -.
DR OrthoDB; 966331at2759; -.
DR PhylomeDB; O49352; -.
DR BioCyc; ARA:AT5G20330-MON; -.
DR PRO; PR:O49352; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O49352; baseline and differential.
DR Genevisible; O49352; AT.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR044965; Glyco_hydro_17_plant.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR32227; PTHR32227; 1.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Plant defense; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..345
FT /note="Probable glucan endo-1,3-beta-glucosidase BG4"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434698"
FT ACT_SITE 128
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT ACT_SITE 267
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 277
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 306
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 22
FT /note="S -> Y (in Ref. 4; AAM62473)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="P -> S (in Ref. 4; AAM62473)"
FT /evidence="ECO:0000305"
FT CONFLICT 126
FT /note="G -> A (in Ref. 4; AAM62473)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="N -> D (in Ref. 4; AAM62473)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="N -> D (in Ref. 4; AAM62473)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 345 AA; 37684 MW; 25B1CDD540297CBE CRC64;
MLYSPKKLFL FFLSCIVLYV NSNNSGFVTA ANSIGLNYGL LGDNLPSPSN VINLYKSIGI
SRIRIFDPNT EVLNALRGHR DIEVTVGVKD QDLAALAASE EAVKGWFAAN IESYLADVNI
TFITVGNEVI PGPIGPQVLP VMQSLTNLVK SRNLPISIST VVAMSNLEQS YPPSAGMFTS
QAREQLVPVL KLLSQTSTPI LVNIYPYFAY ASDPANIRLD YASFNTKSIV VQDGSLGYSN
MFDAIFDAFV WAMEKEGVKN LPMVVSETGW PSAGNGNFTT PAIASTYNRN FVKHIASGKG
TPKRPNKSMN GFLFATFNEN QKPAGTEQNF GLYNPSDMKP IYKLF
