BG5_ARATH
ID BG5_ARATH Reviewed; 354 AA.
AC O49353;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable glucan endo-1,3-beta-glucosidase BG5 {ECO:0000305};
DE EC=3.2.1.39 {ECO:0000305};
DE AltName: Full=Beta-1,3-glucanase 5 {ECO:0000305};
DE Flags: Precursor;
GN Name=BG5 {ECO:0000303|PubMed:10092183};
GN OrderedLocusNames=At5g20340 {ECO:0000312|Araport:AT5G20340,
GN ECO:0000312|EMBL:AED92833.1};
GN ORFNames=F5O24.230 {ECO:0000312|EMBL:AF296825};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10092183; DOI=10.1023/a:1006194822666;
RA Delp G., Palva E.T.;
RT "A novel flower-specific Arabidopsis gene related to both pathogen-induced
RT and developmentally regulated plant beta-1,3-glucanase genes.";
RL Plant Mol. Biol. 39:565-575(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May play a role in plant defense against pathogens.
CC {ECO:0000250|UniProtKB:F4J270}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P33157}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; X79694; CAA56135.1; -; Genomic_DNA.
DR EMBL; AF296825; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92833.1; -; Genomic_DNA.
DR RefSeq; NP_197534.1; NM_122041.2.
DR AlphaFoldDB; O49353; -.
DR SMR; O49353; -.
DR STRING; 3702.AT5G20340.1; -.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR PaxDb; O49353; -.
DR PRIDE; O49353; -.
DR ProteomicsDB; 240824; -.
DR EnsemblPlants; AT5G20340.1; AT5G20340.1; AT5G20340.
DR GeneID; 832156; -.
DR Gramene; AT5G20340.1; AT5G20340.1; AT5G20340.
DR KEGG; ath:AT5G20340; -.
DR Araport; AT5G20340; -.
DR TAIR; locus:2149289; AT5G20340.
DR eggNOG; ENOG502QVKW; Eukaryota.
DR HOGENOM; CLU_024953_0_0_1; -.
DR InParanoid; O49353; -.
DR OMA; YKSIDIT; -.
DR OrthoDB; 966331at2759; -.
DR PhylomeDB; O49353; -.
DR BioCyc; ARA:AT5G20340-MON; -.
DR PRO; PR:O49353; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O49353; baseline and differential.
DR Genevisible; O49353; AT.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004338; F:glucan exo-1,3-beta-glucosidase activity; TAS:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR044965; Glyco_hydro_17_plant.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR32227; PTHR32227; 1.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Plant defense; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..354
FT /note="Probable glucan endo-1,3-beta-glucosidase BG5"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434699"
FT ACT_SITE 137
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT ACT_SITE 276
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT CARBOHYD 286
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 354 AA; 38877 MW; A75D060B6AE16238 CRC64;
MLYLPKKLFL FFFSCIVVIV NYNNSDFVNA ANSIGFVNAA NSIGLNYGLL GDNLPSPSKV
ITLYKSIDIT KIRIFDPNTE VLNALRGHRD IAVTVGVRDQ DLAALSASEE AVKGWFATNI
EPYLSDINIA FITVGNEVIP GPIGPQVLPV MQSLTNLVKS RNLPISISTV VAMWNLEQSY
PPSAGMFTSQ AREQLVPVLK LLSQTNSPIL VKIYPYFSYA SDPSSIRLDY ATFNTEAIVV
QDGSLGYSNM FDAIFDAFVW AMEKEGVKDL PMVVSETGWP SAGNGNITTP DIAGTYNRNF
VKHIASGKGT PKRPNKGIDG FLFATFNENQ KPVGTEQNFG LYNPNDMKPI YNLF
