BGA15_ORYSJ
ID BGA15_ORYSJ Reviewed; 919 AA.
AC Q0INM3; A0A0P0Y9M8; Q2QSG5;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Beta-galactosidase 15;
DE Short=Lactase 15;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN OrderedLocusNames=Os12g0429200, LOC_Os12g24170;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABA97655.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DP000011; ABA97655.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008218; BAF29692.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT16936.1; -; Genomic_DNA.
DR EMBL; AK065546; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q0INM3; -.
DR SMR; Q0INM3; -.
DR STRING; 4530.OS12T0429200-01; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q0INM3; -.
DR PRIDE; Q0INM3; -.
DR EnsemblPlants; Os12t0429200-01; Os12t0429200-01; Os12g0429200.
DR Gramene; Os12t0429200-01; Os12t0429200-01; Os12g0429200.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR InParanoid; Q0INM3; -.
DR OMA; NFFEPFN; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q0INM3; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.740; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..919
FT /note="Beta-galactosidase 15"
FT /id="PRO_0000294167"
FT DOMAIN 822..907
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT ACT_SITE 220
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 289
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 63
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 530
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 546
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 855
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 919 AA; 101006 MW; 84C4826B18470736 CRC64;
MAASRGPPLL GFRALALALL LAILLLLGCS AAAAYAGAEG VLRQVVGRRG DDGGGGNFFE
PFNVTYDHRA VLIGGKRRML VSAGLHYPRA TPEMWPSLIA KCKEGGADVI ETYVFWNGHE
PAKGQYYFEE RFDLVKFAKL VAAEGLFLFL RIGPYACAEW NFGGFPVWLR DIPGIEFRTD
NEPFKAEMQT FVTKIVTLMK EEKLYSWQGG PIILQQIENE YGNIQGNYGQ AGKRYMQWAA
QMAIGLDTGI PWVMCRQTDA PEEIIDTCNA FYCDGFKPNS YNKPTIWTED WDGWYADWGG
ALPHRPAEDS AFAVARFYQR GGSLQNYYMY FGGTNFARTA GGPLQITSYD YDAPIDEYGI
LRQPKWGHLK DLHTAIKLCE PALIAVDGSP QYIKLGSMQE AHVYSTGEVH TNGSMAGNAQ
ICSAFLANID EHKYASVWIF GKSYSLPPWS VSILPDCENV AFNTARIGAQ TSVFTVESGS
PSRSSRHKPS ILSLTSGGPY LSSTWWTSKE TIGTWGGNNF AVQGILEHLN VTKDISDYLW
YTTRVNISDA DVAFWSSKGV LPSLTIDKIR DVARVFVNGK LAGSQVGHWV SLKQPIQLVE
GLNELTLLSE IVGLQNYGAF LEKDGAGFRG QVTLTGLSDG DVDLTNSLWT YQVGLKGEFS
MIYAPEKQGC AGWSRMQKDS VQPFTWYKTM FSTPKGTDPV AIDLGSMGKG QAWVNGHLIG
RYWSLVAPES GCSSSCYYPG AYNERKCQSN CGMPTQNWYH IPREWLKESD NLLVLFEETG
GDPSLISLEA HYAKTVCSRI SENYYPPLSA WSHLSSGRAS VNAATPELRL QCDDGHVISE
ITFASYGTPS GGCLNFSKGN CHASSTLDLV TEACVGNTKC AISVSNDVFG DPCRGVLKDL
AVEAKCSPPS TTKEPRGEM
