BGA17_ARATH
ID BGA17_ARATH Reviewed; 697 AA.
AC Q93Z24; Q2V4C9; Q9SSM8;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Beta-galactosidase 17;
DE Short=Lactase 17;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN Name=BGAL17; OrderedLocusNames=At1g72990; ORFNames=F3N23.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16267099; DOI=10.1093/pcp/pci223;
RA Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.;
RT "Apoplastic glycosidases active against xyloglucan oligosaccharides of
RT Arabidopsis thaliana.";
RL Plant Cell Physiol. 47:55-63(2006).
RN [5]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17466346; DOI=10.1016/j.phytochem.2007.03.021;
RA Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P.,
RA Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 35.";
RL Phytochemistry 68:1510-1520(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93Z24-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93Z24-2; Sequence=VSP_026468;
CC -!- TISSUE SPECIFICITY: Ubiquitous, with higher expression levels in roots
CC and siliques. {ECO:0000269|PubMed:16267099,
CC ECO:0000269|PubMed:17466346}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD55646.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC008017; AAD55646.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35400.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35401.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58944.1; -; Genomic_DNA.
DR EMBL; AY058198; AAL25611.1; -; mRNA.
DR EMBL; AY142008; AAM98272.1; -; mRNA.
DR PIR; C96755; C96755.
DR RefSeq; NP_001031273.1; NM_001036196.2. [Q93Z24-2]
DR RefSeq; NP_001321343.1; NM_001334560.1. [Q93Z24-1]
DR RefSeq; NP_565051.1; NM_105957.3. [Q93Z24-1]
DR AlphaFoldDB; Q93Z24; -.
DR SMR; Q93Z24; -.
DR STRING; 3702.AT1G72990.1; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q93Z24; -.
DR PRIDE; Q93Z24; -.
DR ProteomicsDB; 240657; -. [Q93Z24-1]
DR EnsemblPlants; AT1G72990.1; AT1G72990.1; AT1G72990. [Q93Z24-1]
DR EnsemblPlants; AT1G72990.2; AT1G72990.2; AT1G72990. [Q93Z24-2]
DR EnsemblPlants; AT1G72990.4; AT1G72990.4; AT1G72990. [Q93Z24-1]
DR GeneID; 843630; -.
DR Gramene; AT1G72990.1; AT1G72990.1; AT1G72990. [Q93Z24-1]
DR Gramene; AT1G72990.2; AT1G72990.2; AT1G72990. [Q93Z24-2]
DR Gramene; AT1G72990.4; AT1G72990.4; AT1G72990. [Q93Z24-1]
DR KEGG; ath:AT1G72990; -.
DR Araport; AT1G72990; -.
DR TAIR; locus:2032667; AT1G72990.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_7_2_1; -.
DR InParanoid; Q93Z24; -.
DR OrthoDB; 179316at2759; -.
DR PhylomeDB; Q93Z24; -.
DR BioCyc; ARA:AT1G72990-MON; -.
DR PRO; PR:Q93Z24; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93Z24; baseline and differential.
DR Genevisible; Q93Z24; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR025300; BetaGal_jelly_roll_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF13364; BetaGal_dom4_5; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Apoplast; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..697
FT /note="Beta-galactosidase 17"
FT /id="PRO_0000293096"
FT ACT_SITE 218
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 301
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 333
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 519
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 573
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 583
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 690
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..92
FT /note="MAMTSWPSTGRQRRHQLASMLLLVLVVVGIYVPVFALLPSLSYTPQSLPSAI
FT PQDEKMISRKFYIKDDNFWKDGNRFQIIGGDLHYFRVLPE -> MTISGKMGIVFRSLV
FT VICITFVFFQRLWMQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_026468"
SQ SEQUENCE 697 AA; 78640 MW; 7A13124157BCE54A CRC64;
MAMTSWPSTG RQRRHQLASM LLLVLVVVGI YVPVFALLPS LSYTPQSLPS AIPQDEKMIS
RKFYIKDDNF WKDGNRFQII GGDLHYFRVL PEYWEDRLLR ANALGLNTIQ VYVPWNLHEP
KPGKMVFEGI GDLVSFLKLC EKLDFLVMLR AGPYICGEWD LGGFPAWLLA VKPRLQLRTS
DPVYLKLVER WWDVLLPKVF PLLYSNGGPV IMVQIENEYG SYGNDKAYLR KLVSMARGHL
GDDIIVYTTD GGTKETLDKG TVPVADVYSA VDFSTGDDPW PIFKLQKKFN APGRSPPLSS
EFYTGWLTHW GEKITKTDAE FTAASLEKIL SRNGSAVLYM VHGGTNFGFY NGANTGSEES
DYKPDLTSYD YDAPIKESGD IDNPKFQALQ RVIKKYNASP HPISPSNKQR KAYGSIKMQM
TTSLFDLVRM TDPADVITSA NPISMESVGQ MFGFLLYESS YIAKKSGNTL RIPKVHDRAQ
VFVSCLSQDV DVGVLRYIGT TERWNNQPIS LPTIECTTNT SLFILVENMG RVNYGPYIFD
DKGILSSVYL DGQILHGWKM IPIPFHNLNQ EPNLTFEMQH TKNRSKKFEL TNDVGRKEPA
LFAGEFSINS EEEIKDTYLS FNGWGKGVAF VNEFNIGRYW PSVGPQCNLY VPAPLLKRGK
NTLVVFELES PHLELSLEAV DHQDFTCGSN VSKVNQL
