BGAL1_ORYSJ
ID BGAL1_ORYSJ Reviewed; 827 AA.
AC Q8RUV9; B7F4V2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Beta-galactosidase 1;
DE Short=Lactase 1;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN OrderedLocusNames=Os01g0533400, LOC_Os01g34920;
GN ORFNames=OJ1029_F04.5, OJ1619_F12.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
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DR EMBL; AP003445; BAB89138.1; -; Genomic_DNA.
DR EMBL; AP003447; BAB90329.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05166.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72526.1; -; Genomic_DNA.
DR EMBL; AK119447; BAG99649.1; -; mRNA.
DR RefSeq; XP_015621617.1; XM_015766131.1.
DR AlphaFoldDB; Q8RUV9; -.
DR SMR; Q8RUV9; -.
DR STRING; 4530.OS01T0533400-01; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q8RUV9; -.
DR PRIDE; Q8RUV9; -.
DR EnsemblPlants; Os01t0533400-01; Os01t0533400-01; Os01g0533400.
DR GeneID; 4327808; -.
DR Gramene; Os01t0533400-01; Os01t0533400-01; Os01g0533400.
DR KEGG; osa:4327808; -.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR InParanoid; Q8RUV9; -.
DR OMA; QFFRTES; -.
DR OrthoDB; 179316at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q8RUV9; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..827
FT /note="Beta-galactosidase 1"
FT /id="PRO_0000294152"
FT DOMAIN 746..827
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT ACT_SITE 187
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 259
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 249
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 260
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 366
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 392
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 502
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 578
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 586
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 615
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 827 AA; 91711 MW; 749E7C4D35FEA80B CRC64;
MMGRRGSSWC RWWVALLVLA VAADAVGCTS VSYDDRSLVI DGQRRIILSG SIHYPRSTPE
MWPDLIKKAK EGGLDAIETY IFWNGHEPHR RQYNFEGNYD VVRFFKEIQN AGMYAILRIG
PYICGEWNYG GLPAWLRDIP GMQFRLHNEP FENEMETFTT LIVNKMKDSK MFAEQGGPII
LAQIENEYGN IMGKLNNNQS ASEYIHWCAD MANKQNVGVP WIMCQQDDDV PHNVVNTCNG
FYCHDWFPNR TGIPKIWTEN WTGWFKAWDK PDFHRSAEDI AFAVAMFFQK RGSLQNYYMY
HGGTNFGRTS GGPYITTSYD YDAPLDEYGN LRQPKYGHLK ELHSVLKSME KTLVHGEYFD
TNYGDNITVT KYTLDSSSAC FINNRFDDKD VNVTLDGATH LLPAWSVSIL PDCKTVAFNS
AKIKTQTSVM VKKPNTAEQE QESLKWSWMP ENLSPFMTDE KGNFRKNELL EQIVTSTDQS
DYLWYRTSLN HKGEGSYKLY VNTTGHELYA FVNGKLIGKN HSADGDFVFQ LESPVKLHDG
KNYISLLSAT VGLKNYGPSF EKMPTGIVGG PVKLIDSNGT AIDLSNSSWS YKAGLASEYR
QIHLDKPGYK WNGNNGTIPI NRPFTWYKAT FEAPSGEDAV VVDLLGLNKG VAWVNGNNLG
RYWPSYTAAE MAGCHRCDYR GAFQAEGDGT RCLTGCGEPS QRYYHVPRSF LAAGEPNTLL
LFEEAGGDPS GVALRTVVPG AVCTSGEAGD AVTLSCGGGH AVSSVDVASF GVGRGRCGGY
EGGCESKAAY EAFTAACVGK ESCTVEITGA FAGAGCLSGV LTVQATC
