BGAL2_ORYSJ
ID BGAL2_ORYSJ Reviewed; 827 AA.
AC Q8W0A1; B7EU06;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Beta-galactosidase 2;
DE Short=Lactase 2;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN OrderedLocusNames=Os01g0580200, LOC_Os01g39830; ORFNames=P0672C09.21;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
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DR EMBL; AP003546; BAB84455.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05319.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72858.1; -; Genomic_DNA.
DR EMBL; AK103045; BAG95853.1; -; mRNA.
DR RefSeq; XP_015621619.1; XM_015766133.1.
DR AlphaFoldDB; Q8W0A1; -.
DR SMR; Q8W0A1; -.
DR STRING; 4530.OS01T0580200-01; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q8W0A1; -.
DR PRIDE; Q8W0A1; -.
DR EnsemblPlants; Os01t0580200-01; Os01t0580200-01; Os01g0580200.
DR GeneID; 4324191; -.
DR Gramene; Os01t0580200-01; Os01t0580200-01; Os01g0580200.
DR KEGG; osa:4324191; -.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR InParanoid; Q8W0A1; -.
DR OMA; QSYNDEP; -.
DR OrthoDB; 179316at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q8W0A1; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.740; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..827
FT /note="Beta-galactosidase 2"
FT /id="PRO_0000294153"
FT DOMAIN 741..827
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT ACT_SITE 182
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 251
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 209
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 458
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 827 AA; 91549 MW; 100D0225FF558261 CRC64;
MAASAVAVAF VVAVAAVLAA AASAAVTYDR KAVVVNGQRR ILISGSIHYP RSTPEMWPDL
IEKAKDGGLD VVQTYVFWNG HEPSPGQYYF EGRYDLVHFI KLVKQAGLYV NLRIGPYVCA
EWNFGGFPVW LKYVPGISFR TDNEPFKAEM QKFTTKIVEM MKSEGLFEWQ GGPIILSQIE
NEFGPLEWDQ GEPAKAYASW AANMAVALNT SVPWIMCKED DAPDPIINTC NGFYCDWFSP
NKPHKPTMWT EAWTAWYTGF GIPVPHRPVE DLAYGVAKFI QKGGSFVNYY MYHGGTNFGR
TAGGPFIATS YDYDAPIDEY GLLREPKWGH LKQLHKAIKL CEPALVAGDP IVTSLGNAQK
SSVFRSSTGA CAAFLENKDK VSYARVAFNG MHYDLPPWSI SILPDCKTTV FNTARVGSQI
SQMKMEWAGG FAWQSYNEEI NSFGEDPLTT VGLLEQINVT RDNTDYLWYT TYVDVAQDEQ
FLSNGENLKL TVMSAGHALH IFINGQLKGT VYGSVDDPKL TYTGNVKLWA GSNTISCLSI
AVGLPNVGEH FETWNAGILG PVTLDGLNEG RRDLTWQKWT YQVGLKGESM SLHSLSGSST
VEWGEPVQKQ PLTWYKAFFN APDGDEPLAL DMSSMGKGQI WINGQGIGRY WPGYKASGNC
GTCDYRGEYD ETKCQTNCGD SSQRWYHVPR SWLSPTGNLL VIFEEWGGDP TGISMVKRSI
GSVCADVSEW QPSMKNWHTK DYEKAKVHLQ CDNGQKITEI KFASFGTPQG SCGSYTEGGC
HAHKSYDIFW KNCVGQERCG VSVVPEIFGG DPCPGTMKRA VVEAICG
