ID   BGAL4_ORYSI             Reviewed;         729 AA.
AC   A2X2H7;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Beta-galactosidase 4;
DE            Short=Lactase 4;
DE            EC=3.2.1.23;
DE   Flags: Precursor;
GN   ORFNames=OsI_006270;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
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DR   EMBL; CM000127; EAY85037.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2X2H7; -.
DR   SMR; A2X2H7; -.
DR   STRING; 39946.A2X2H7; -.
DR   EnsemblPlants; BGIOSGA006862-TA; BGIOSGA006862-PA; BGIOSGA006862.
DR   Gramene; BGIOSGA006862-TA; BGIOSGA006862-PA; BGIOSGA006862.
DR   HOGENOM; CLU_007853_4_0_1; -.
DR   OMA; MNSAVKV; -.
DR   Proteomes; UP000007015; Chromosome 2.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   3: Inferred from homology;
KW   Apoplast; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..729
FT                   /note="Beta-galactosidase 4"
FT                   /id="PRO_0000294155"
FT   ACT_SITE        194
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        263
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   729 AA;  80095 MW;  190E85CECA84E8CE CRC64;
     MAPAPTPAAA AGRRVAVLAA ALVAASLAAS VGVANAAVSY DRRSLVINGR RRILLSGSIH
     YPRSTPEMWP GLIQKAKDGG LDVIQTYVFW NGHEPVQGQY YFSDRYDLVR FVKLVKQAGL
     YVHLRIGPYV CAEWNFGGFP VWLKYVPGVS FRTDNGPFKA EMQKFVEKIV SMMKSEGLFE
     WQGGPIIMSQ VENEFGPMES VGGSGAKPYA NWAAKMAVRT NTGVPWVMCK QDDAPDPVIN
     TCNGFYCDYF SPNKNYKPSM WTEAWTGWFT SFGGGVPHRP VEDLAFAVAR FIQKGGSFVN
     YYMYHGGTNF GRTAGGPFIA TSYDYDAPID EFGLLRQPKW GHLRDLHRAI KQAEPVLVSA
     DPTIESIGSY EKAYVFKAKN GACAAFLSNY HMNTAVKVRF NGQQYNLPAW SISILPDCKT
     AVFNTATVKE PTLMPKMNPV VRFAWQSYSE DTNSLSDSAF TKDGLVEQLS MTWDKSDYLW
     YTTYVNIGTN DLRSGQSPQL TVYSAGHSMQ VFVNGKSYGS VYGGYDNPKL TYNGRVKMWQ
     GSNKISILSS AVGLPNVGNH FENWNVGVLG PVTLSSLNGG TKDLSHQKWT YQVGLKGETL
     GLHTVTGSSA VEWGGPGGYQ PLTWHKAFFN APAGNDPVAL DMGSMGKGQL WVNGHHVGRY
     WSYKASGGCG GCSYAGTYHE DKCRSNCGDL SQRWYHVPRS WLKPGGNLLV VLEEYGGDLA
     GVSLATRTT