ID   BGAL4_ORYSJ             Reviewed;         729 AA.
AC   Q6Z6K4; Q0E2R4;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Beta-galactosidase 4;
DE            Short=Lactase 4;
DE            EC=3.2.1.23;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os02g0219200, LOC_Os02g12730; ORFNames=P0027A02.24;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 230-729.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF08224.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP004996; BAD17189.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08224.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK059059; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015627101.1; XM_015771615.1.
DR   AlphaFoldDB; Q6Z6K4; -.
DR   SMR; Q6Z6K4; -.
DR   STRING; 4530.OS02T0219200-01; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   PaxDb; Q6Z6K4; -.
DR   PRIDE; Q6Z6K4; -.
DR   GeneID; 4328745; -.
DR   KEGG; osa:4328745; -.
DR   eggNOG; KOG0496; Eukaryota.
DR   InParanoid; Q6Z6K4; -.
DR   OrthoDB; 179316at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..729
FT                   /note="Beta-galactosidase 4"
FT                   /id="PRO_0000294156"
FT   ACT_SITE        194
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        263
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   729 AA;  79996 MW;  877E85CECA84EEEE CRC64;
     MAPAPTPAAA AGRRVAVLAA ALVAASLAAS VGVANAAVSY DRRSLVINGR RRILLSGSIH
     YPRSTPEMWP GLIQKAKDGG LDVIQTYVFW NGHEPVQGQY YFSDRYDLVR FVKLVKQAGL
     YVHLRIGPYV CAEWNFGGFP VWLKYVPGVS FRTDNGPFKA EMQKFVEKIV SMMKSEGLFE
     WQGGPIIMSQ VENEFGPMES VGGSGAKPYA NWAAKMAVGT NTGVPWVMCK QDDAPDPVIN
     TCNGFYCDYF SPNKNYKPSM WTEAWTGWFT SFGGGVPHRP VEDLAFAVAR FIQKGGSFVN
     YYMYHGGTNF GRTAGGPFIA TSYDYDAPID EFGLLRQPKW GHLRDLHRAI KQAEPVLVSA
     DPTIESIGSY EKAYVFKAKN GACAAFLSNY HMNTAVKVRF NGQQYNLPAW SISILPDCKT
     AVFNTATVKE PTLMPKMNPV VRFAWQSYSE DTNSLSDSAF TKDGLVEQLS MTWDKSDYLW
     YTTYVNIGTN DLRSGQSPQL TVYSAGHSMQ VFVNGKSYGS VYGGYDNPKL TYNGRVKMWQ
     GSNKISILSS AVGLPNVGNH FENWNVGVLG PVTLSSLNGG TKDLSHQKWT YQVGLKGETL
     GLHTVTGSSA VEWGGPGGYQ PLTWHKAFFN APAGNDPVAL DMGSMGKGQL WVNGHHVGRY
     WSYKASGGCG GCSYAGTYHE DKCRSNCGDL SQRWYHVPRS WLKPGGNLLV VLEEYGGDLA
     GVSLATRTT