BGAL5_ORYSJ
ID BGAL5_ORYSJ Reviewed; 841 AA.
AC Q10RB4; B7EST9; Q8L3P5;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Beta-galactosidase 5;
DE Short=Lactase 5;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN OrderedLocusNames=Os03g0165400, LOC_Os03g06940; ORFNames=OJ1123F12.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed;
RA Chantarangsee M., Mizuno K., Fujimura T., Ketudat-Cairns J.R.;
RT "Cloning and expression of beta-galactosidase from rice.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
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DR EMBL; AF499737; AAM22973.1; -; mRNA.
DR EMBL; AF508799; AAM34271.1; -; mRNA.
DR EMBL; DP000009; ABF94149.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10982.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82468.1; -; Genomic_DNA.
DR EMBL; AK102192; BAG95436.1; -; mRNA.
DR RefSeq; XP_015633043.1; XM_015777557.1.
DR AlphaFoldDB; Q10RB4; -.
DR SMR; Q10RB4; -.
DR STRING; 4530.OS03T0165400-01; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q10RB4; -.
DR PRIDE; Q10RB4; -.
DR EnsemblPlants; Os03t0165400-01; Os03t0165400-01; Os03g0165400.
DR GeneID; 4331730; -.
DR Gramene; Os03t0165400-01; Os03t0165400-01; Os03g0165400.
DR KEGG; osa:4331730; -.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR InParanoid; Q10RB4; -.
DR OMA; KAYINWA; -.
DR OrthoDB; 179316at2759; -.
DR BRENDA; 3.2.1.23; 4460.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q10RB4; baseline and differential.
DR Genevisible; Q10RB4; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.740; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..841
FT /note="Beta-galactosidase 5"
FT /id="PRO_0000294157"
FT DOMAIN 752..838
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT ACT_SITE 183
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 252
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 392
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 461
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 475
FT /note="S -> R (in Ref. 1; AAM22973/AAM34271)"
FT /evidence="ECO:0000305"
FT CONFLICT 585
FT /note="Q -> QFQ (in Ref. 1; AAM22973/AAM34271)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 841 AA; 92172 MW; 2D35B96E6CB7C250 CRC64;
MGRGCLAALL GGAVAVAVLV AVVHCAVTYD KKAVLVDGQR RILFSGSIHY PRSTPEMWDG
LIEKAKDGGL DVIQTYVFWN GHEPTPGNYN FEGRYDLVRF IKTVQKAGMF VHLRIGPYIC
GEWNFGGFPV WLKYVPGISF RTDNEPFKNA MQGFTEKIVG MMKSENLFAS QGGPIILSQI
ENEYGPEGKE FGAAGKAYIN WAAKMAVGLD TGVPWVMCKE DDAPDPVINA CNGFYCDTFS
PNKPYKPTMW TEAWSGWFTE FGGTIRQRPV EDLAFGVARF VQKGGSFINY YMYHGGTNFG
RTAGGPFITT SYDYDAPLDE YGLAREPKFG HLKELHRAVK LCEQPLVSAD PTVTTLGSMQ
EAHVFRSSSG CAAFLANYNS NSYAKVIFNN ENYSLPPWSI SILPDCKNVV FNTATVGVQT
NQMQMWADGA SSMMWEKYDE EVDSLAAAPL LTSTGLLEQL NVTRDTSDYL WYITSVEVDP
SEKFLQGGTP LSLTVQSAGH ALHVFINGQL QGSAYGTRED RKISYSGNAN LRAGTNKVAL
LSVACGLPNV GVHYETWNTG VVGPVVIHGL DEGSRDLTWQ TWSYQVGLKG EQMNLNSLEG
SGSVEWMQGS LVAQNQQPLA WYRAYFDTPS GDEPLALDMG SMGKGQIWIN GQSIGRYWTA
YAEGDCKGCH YTGSYRAPKC QAGCGQPTQR WYHVPRSWLQ PTRNLLVVFE ELGGDSSKIA
LAKRTVSGVC ADVSEYHPNI KNWQIESYGE PEFHTAKVHL KCAPGQTISA IKFASFGTPL
GTCGTFQQGE CHSINSNSVL EKKCIGLQRC VVAISPSNFG GDPCPEVMKR VAVEAVCSTA
A
