BGAL6_ARATH
ID BGAL6_ARATH Reviewed; 718 AA.
AC Q9FFN4; Q0WMN2; Q9SCV6;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Beta-galactosidase 6;
DE Short=Lactase 6;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN Name=BGAL6; OrderedLocusNames=At5g63800; ORFNames=MBK5.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Gy I., Kreis M., Lecharny A.;
RT "The beta-galactosidases are encoding by a multigene family in Arabidopsis
RT thaliana.";
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16267099; DOI=10.1093/pcp/pci223;
RA Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.;
RT "Apoplastic glycosidases active against xyloglucan oligosaccharides of
RT Arabidopsis thaliana.";
RL Plant Cell Physiol. 47:55-63(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17466346; DOI=10.1016/j.phytochem.2007.03.021;
RA Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P.,
RA Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 35.";
RL Phytochemistry 68:1510-1520(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in siliques.
CC {ECO:0000269|PubMed:16267099}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB64742.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ270302; CAB64742.1; ALT_FRAME; mRNA.
DR EMBL; AB005234; BAB10473.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97799.1; -; Genomic_DNA.
DR EMBL; AK229786; BAF01618.1; -; mRNA.
DR RefSeq; NP_568978.2; NM_125775.4.
DR AlphaFoldDB; Q9FFN4; -.
DR SMR; Q9FFN4; -.
DR STRING; 3702.AT5G63800.1; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q9FFN4; -.
DR PRIDE; Q9FFN4; -.
DR ProteomicsDB; 240779; -.
DR EnsemblPlants; AT5G63800.1; AT5G63800.1; AT5G63800.
DR GeneID; 836500; -.
DR Gramene; AT5G63800.1; AT5G63800.1; AT5G63800.
DR KEGG; ath:AT5G63800; -.
DR Araport; AT5G63800; -.
DR TAIR; locus:2160649; AT5G63800.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR InParanoid; Q9FFN4; -.
DR OMA; INNWKAF; -.
DR OrthoDB; 179316at2759; -.
DR PhylomeDB; Q9FFN4; -.
DR BioCyc; ARA:AT5G63800-MON; -.
DR PRO; PR:Q9FFN4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFN4; baseline and differential.
DR Genevisible; Q9FFN4; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IDA:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0048354; P:mucilage biosynthetic process involved in seed coat development; IMP:TAIR.
DR GO; GO:0009827; P:plant-type cell wall modification; IMP:TAIR.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..718
FT /note="Beta-galactosidase 6"
FT /id="PRO_5000065881"
FT ACT_SITE 188
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 259
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 468
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 538
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 70
FT /note="T -> A (in Ref. 4; BAF01618)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 718 AA; 79759 MW; 3AE3F384A592A5D4 CRC64;
MEMGRLVFGL CLILIVGTFL EFSGGATAAK GVTYDGRSLI IDGQRKLLFS GSIHYPRSTP
EMWPSLIKKT KEGGIDVIQT YVFWNLHEPK LGQYDFSGRN DLVKFIKEIR SQGLYVCLRI
GPFIEAEWNY GGLPFWLRDV PGMVYRTDNE PFKFHMQKFT AKIVDLMKSE GLYASQGGPI
ILSQIENEYA NVEGAFHEKG ASYIKWAGQM AVGLKTGVPW IMCKSPDAPD PVINTCNGMK
CGETFPGPNS PNKPKMWTED WTSFFQVYGK EPYIRSAEDI AFHAALFVAK NGSYINYYMY
HGGTNFGRTS SSYFITGYYD QAPLDEYGLL RQPKYGHLKE LHAAIKSSAN PLLQGKQTIL
SLGPMQQAYV FEDANNGCVA FLVNNDAKAS QIQFRNNAYS LSPKSIGILQ NCKNLIYETA
KVNVKMNTRV TTPVQVFNVP DNWNLFRETI PAFPGTSLKT NALLEHTNLT KDKTDYLWYT
SSFKLDSPCT NPSIYTESSG HVVHVFVNNA LAGSGHGSRD IRVVKLQAPV SLINGQNNIS
ILSGMVGLPD SGAYMERRSY GLTKVQISCG GTKPIDLSRS QWGYSVGLLG EKVRLYQWKN
LNRVKWSMNK AGLIKNRPLA WYKTTFDGPN GDGPVGLHMS SMGKGEIWVN GESIGRYWVS
FLTPAGQPSQ SIYHIPRAFL KPSGNLLVVF EEEGGDPLGI SLNTISVVGS SQAQSQFS
