BGAL6_ORYSJ
ID BGAL6_ORYSJ Reviewed; 858 AA.
AC Q10NX8;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Beta-galactosidase 6;
DE Short=Lactase 6;
DE EC=3.2.1.23;
DE Flags: Precursor;
GN OrderedLocusNames=Os03g0255100, LOC_Os03g15020;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP PROTEIN SEQUENCE OF 31-80 AND 453-498, FUNCTION, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=cv. Nipponbare; TISSUE=Callus;
RX PubMed=12723614; DOI=10.1271/bbb.67.627;
RA Kaneko S., Kobayashi H.;
RT "Purification and characterization of extracellular beta-galactosidase
RT secreted by supension cultured rice (Oryza sativa L.) cells.";
RL Biosci. Biotechnol. Biochem. 67:627-630(2003).
CC -!- FUNCTION: Releases galactose by hydrolysis of plant cell wall
CC galactose-containing polysaccharides such as galacto-xyloglucan, pectic
CC galactan and galactan (in vitro). {ECO:0000269|PubMed:12723614}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 3.5. {ECO:0000269|PubMed:12723614};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF95027.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF11505.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DP000009; ABF95027.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008209; BAF11505.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015632058.1; XM_015776572.1.
DR AlphaFoldDB; Q10NX8; -.
DR SMR; Q10NX8; -.
DR STRING; 4530.OS03T0255100-01; -.
DR CAZy; GH35; Glycoside Hydrolase Family 35.
DR PaxDb; Q10NX8; -.
DR PRIDE; Q10NX8; -.
DR GeneID; 4332289; -.
DR KEGG; osa:4332289; -.
DR eggNOG; KOG0496; Eukaryota.
DR InParanoid; Q10NX8; -.
DR OrthoDB; 179316at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10NX8; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.740; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421; PTHR23421; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 1: Evidence at protein level;
KW Apoplast; Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000269|PubMed:12723614"
FT CHAIN 31..858
FT /note="Beta-galactosidase 6"
FT /id="PRO_0000294158"
FT DOMAIN 772..858
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT ACT_SITE 189
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 258
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 482
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 507
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 595
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 830
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 79
FT /note="I -> F (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 858 AA; 92840 MW; DBD15B3FB1FF8067 CRC64;
MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV SGSIHYPRST
PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR KDLVRFVKAV ADAGLYVHLR
IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN EAFKAEMQRF TEKVVDTMKG AGLYASQGGP
IILSQIENEY GNIDSAYGAA GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF
YCDQFTPNSK SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH
GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP ALIAAEPSYS
SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT YKLPAWSVSI LPDCKNVVLN
TAQINSQVTT SEMRSLGSSI QDTDDSLITP ELATAGWSYA IEPVGITKEN ALTKPGLMEQ
INTTADASDF LWYSTSIVVK GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS
SLISLQTPVT LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD
WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD PVAIDFTGMG
KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC LKKCGQPSQT LYHVPRSFLQ
PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC AHVSEMHPAQ IDSWISPQQT SQTQGPALRL
ECPREGQVIS NIKFASFGTP SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF
GDPCSGVTKS LVVEAACS
