ID   BGAL9_ORYSJ             Reviewed;         715 AA.
AC   Q5Z7L0; A0A0P0WY17;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Beta-galactosidase 9;
DE            Short=Lactase 9;
DE            EC=3.2.1.23;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os06g0573600, LOC_Os06g37560;
GN   ORFNames=OSJNBa0006A22.31-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
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DR   EMBL; AP004729; BAD61846.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19834.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS98330.1; -; Genomic_DNA.
DR   EMBL; AK102756; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015644212.1; XM_015788726.1.
DR   AlphaFoldDB; Q5Z7L0; -.
DR   SMR; Q5Z7L0; -.
DR   STRING; 4530.OS06T0573600-01; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   PaxDb; Q5Z7L0; -.
DR   PRIDE; Q5Z7L0; -.
DR   EnsemblPlants; Os06t0573600-01; Os06t0573600-01; Os06g0573600.
DR   GeneID; 4341358; -.
DR   Gramene; Os06t0573600-01; Os06t0573600-01; Os06g0573600.
DR   KEGG; osa:4341358; -.
DR   eggNOG; KOG0496; Eukaryota.
DR   HOGENOM; CLU_007853_4_2_1; -.
DR   InParanoid; Q5Z7L0; -.
DR   OMA; GTHYETW; -.
DR   OrthoDB; 179316at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q5Z7L0; baseline and differential.
DR   Genevisible; Q5Z7L0; OS.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..715
FT                   /note="Beta-galactosidase 9"
FT                   /id="PRO_0000294161"
FT   ACT_SITE        178
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        247
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        26
FT                   /note="H -> R (in Ref. 4; AK102756)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   715 AA;  78280 MW;  828368E0725B61B2 CRC64;
     MSGGAVAFLL LVAAAAVANA AVTYDHRSLT INGQRRILIS GSIHYPRSTP EMWPDLIQKA
     KDGGLDVIQT YVFWNGHEPV QGQYYFSDRY DLVRFVKLVK QAGLYVNLRI GPYVCAEWNY
     GGFPVWLKYV PGISFRTDNG PFKAAMQTFV EKIVSMMKSE GLFEWQGGPI ILAQVENEYG
     PMESVMGSGA KSYVDWAAKM AVATNAGVPW IMCKQDDAPD PVINTCNGFY CDDFTPNSKN
     KPSMWTEAWS GWFTAFGGTV PQRPVEDLAF AVARFIQKGG SFINYYMYHG GTNFDRTAGG
     PFIATSYDYD APIDEYGLLR QPKWGHLTNL HKAIKQAETA LVAGDPTVQN IGNYEKAYVF
     RSSSGDCAAF LSNFHTSAAA RVAFNGRRYD LPAWSISVLP DCRTAVYNTA TVTAASSPAK
     MNPAGGFTWQ SYGEATNSLD ETAFTKDGLV EQLSMTWDKS DYLWYTTYVN IDSGEQFLKS
     GQWPQLTVYS AGHSVQVFVN GQYFGNAYGG YDGPKLTYSG YVKMWQGSNK ISILSSAVGL
     PNVGTHYETW NIGVLGPVTL SGLNEGKRDL SKQKWTYQIG LKGEKLGVHS VSGSSSVEWG
     GAAGKQPVTW HRAYFNAPAG GAPVALDLGS MGKGQAWVNG HLIGRYWSYK ASGNCGGCSY
     AGTYSEKKCQ ANCGDASQRW YHVPRSWLNP SGNLVVLLEE FGGDLSGVTL MTRTT