ID   SECF_VIBAL              Reviewed;         315 AA.
AC   E9RGS4;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Protein translocase subunit SecF;
GN   Name=secF;
OS   Vibrio alginolyticus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=138-2;
RA   Tsukazaki T., Mori H., Echizen Y., Ishitani R., Fukai S., Tanaka T.,
RA   Sasaki Y., Mio K., Kuwata M., Perederina A., Vassylyev D.G., Kohno T.,
RA   Sato C., Maturana A., Ito K., Nureki O.;
RT   "Structure and function of SecDF, a protein export-enhancing membrane
RT   component.";
RL   Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN E.COLI, AND FUNCTION IN PROTEIN TRANSLOCATION.
RC   STRAIN=138-2;
RX   PubMed=21562494; DOI=10.1038/nature09980;
RA   Tsukazaki T., Mori H., Echizen Y., Ishitani R., Fukai S., Tanaka T.,
RA   Perederina A., Vassylyev D.G., Kohno T., Maturana A.D., Ito K., Nureki O.;
RT   "Structure and function of a membrane component SecDF that enhances protein
RT   export.";
RL   Nature 474:235-238(2011).
CC   -!- FUNCTION: Expression of V.alginolyticus SecDF in an E.coli secDF mutant
CC       restores protein export in a Na(+)-dependent manner, strongly
CC       suggesting SecDF functions via cation-coupled protein translocation.
CC       {ECO:0000269|PubMed:21562494}.
CC   -!- FUNCTION: Part of the Sec protein translocase complex. Interacts with
CC       the SecYEG preprotein conducting channel. SecDF uses the proton motive
CC       force (PMF) to complete protein translocation after the ATP-dependent
CC       function of SecA (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with SecD. Part of the essential Sec protein
CC       translocation apparatus which comprises SecA, SecYEG and auxiliary
CC       proteins SecDF-YajC and YidC (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SecD/SecF family. SecF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB583187; BAJ78590.1; -; Genomic_DNA.
DR   RefSeq; WP_005391572.1; NZ_PZOD01000080.1.
DR   AlphaFoldDB; E9RGS4; -.
DR   SMR; E9RGS4; -.
DR   STRING; 663.BAU10_01935; -.
DR   eggNOG; COG0341; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015450; F:protein-transporting ATPase activity; IEA:InterPro.
DR   GO; GO:0065002; P:intracellular protein transmembrane transport; IEA:UniProtKB-UniRule.
DR   GO; GO:0006605; P:protein targeting; IEA:UniProtKB-UniRule.
DR   GO; GO:0043952; P:protein transport by the Sec complex; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01464_B; SecF_B; 1.
DR   InterPro; IPR022813; SecD/SecF_arch_bac.
DR   InterPro; IPR022645; SecD/SecF_bac.
DR   InterPro; IPR022646; SecD/SecF_CS.
DR   InterPro; IPR005665; SecF_bac.
DR   PANTHER; PTHR30081; PTHR30081; 1.
DR   Pfam; PF07549; Sec_GG; 1.
DR   Pfam; PF02355; SecD_SecF; 1.
DR   PRINTS; PR01755; SECFTRNLCASE.
DR   TIGRFAMs; TIGR00916; 2A0604s01; 1.
DR   TIGRFAMs; TIGR00966; 3a0501s07; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Protein transport; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..315
FT                   /note="Protein translocase subunit SecF"
FT                   /id="PRO_0000412707"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        270..290
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   315 AA;  34414 MW;  0709588543202498 CRC64;
     MFQILKAEKT IGFMRWSKVA FVFSIFMIAA SIFTLSTKWL NWGLDFTGGT LIEVGFEKPA
     NLEKIRTALD AKGFGDATVQ NFGSAREVMV RLRPRDDVSG ETLGNQIIGA IKDGTGESVE
     MRRIEFVGPN VGDELTEAGG LAILVSLICI LLYVSMRFEW RLAAGAVMAL AHDIIITLGV
     FSFLQIEVDL TIVAALLTVV GYSLNDTIVV FDRIRENFRK MRKGEPADIM DASITQTLSR
     TLITSGTTLF VVIALFMQGG AMIHGFATAL LLGITVGTYS SIYVASALAL KLGIQKEHLM
     PPQVEKEGAE FDEMP