ID   SECY_GUITH              Reviewed;         420 AA.
AC   P28527;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Protein translocase subunit SecY;
GN   Name=secY;
OS   Guillardia theta (Cryptophyte) (Cryptomonas phi).
OG   Plastid; Chloroplast.
OC   Eukaryota; Cryptophyceae; Pyrenomonadales; Geminigeraceae; Guillardia.
OX   NCBI_TaxID=55529;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1544427; DOI=10.1016/0014-5793(92)80029-g;
RA   Douglas S.E.;
RT   "A secY homologue is found in the plastid genome of Cryptomonas phi.";
RL   FEBS Lett. 298:93-96(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9137835; DOI=10.1080/15216549700202101;
RA   Wang S.L., Liu X.-Q., Douglas S.E.;
RT   "The large ribosomal protein gene cluster of a cryptomonad plastid: gene
RT   organization, sequence and evolutionary implications.";
RL   Biochem. Mol. Biol. Int. 41:1035-1044(1997).
CC   -!- FUNCTION: The central subunit of the protein translocation channel
CC       SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two
CC       domains form a lateral gate at the front which open onto the bilayer
CC       between TMs 2 and 7, and are clamped together by SecE at the back. The
CC       channel is closed by both a pore ring composed of hydrophobic SecY
CC       resides and a short helix (helix 2A) on the extracellular side of the
CC       membrane which forms a plug (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the plastid Sec protein translocase complex,
CC       which is composed of at least SecY, SecE and SecG. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SecY/SEC61-alpha family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF041468; AAC35720.1; -; Genomic_DNA.
DR   RefSeq; NP_050786.1; NC_000926.1.
DR   AlphaFoldDB; P28527; -.
DR   SMR; P28527; -.
DR   GeneID; 857094; -.
DR   HOGENOM; CLU_030313_0_0_1; -.
DR   OMA; FAMWLGE; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0065002; P:intracellular protein transmembrane transport; IEA:UniProtKB-UniRule.
DR   GO; GO:0006605; P:protein targeting; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3370.10; -; 1.
DR   HAMAP; MF_01465; SecY; 1.
DR   InterPro; IPR026593; SecY.
DR   InterPro; IPR002208; SecY/SEC61-alpha.
DR   InterPro; IPR030659; SecY_CS.
DR   InterPro; IPR023201; SecY_dom_sf.
DR   PANTHER; PTHR10906; PTHR10906; 1.
DR   Pfam; PF00344; SecY; 1.
DR   PIRSF; PIRSF004557; SecY; 1.
DR   SUPFAM; SSF103491; SSF103491; 1.
DR   TIGRFAMs; TIGR00967; 3a0501s007; 1.
DR   PROSITE; PS00755; SECY_1; 1.
DR   PROSITE; PS00756; SECY_2; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Protein transport; Thylakoid;
KW   Translocation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..420
FT                   /note="Protein translocase subunit SecY"
FT                   /id="PRO_0000131775"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   420 AA;  46937 MW;  7967611DB9EF59F6 CRC64;
     MNTSIKSIKK QDLKDRIVFT LFLIVMSRLG TFLPIPGVDH DAFYQSIISN PLVNFLNVFS
     GGGFASIGVF ALGIVPYINA SIIVQLATNS IPSLEKLQKE EGELGRQKIV QLTRYVALVW
     ALIQSIGVSF WVRPYVFNWD LNFVFAMSLT LTIGSMLIMW FSEQITEKGI GNGPSLLIFI
     NIISGLPKLL QSQIQSTRLN IQALDIFVLV FIFSVMIIGI IFIQEGIKRI PIISARQLGK
     GQMDNKTSYL PLKLNQSGVM PIIFASAVLV LPAYLAQLVS NEQLRTVLHL FDGTSNNKLL
     YLLFYFTLIL FFSYFYTSLI LNPNDVSKNL KKMESSIYGV RPGKATTEYL QKTLNRLTFL
     GALFLAFIAI VPNIIETLTN LSVFKGLGGT SLLIIVGVQV DTSKQIQTYL ISKNYETIVR