SECY_ORYSJ
ID SECY_ORYSJ Reviewed; 556 AA.
AC Q6ZG25; A0A0P0XDU3; Q0J6X0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Preprotein translocase subunit SECY, chloroplastic;
DE AltName: Full=CpSecY;
DE Flags: Precursor;
GN Name=SECY;
GN OrderedLocusNames=Os08g0254500 {ECO:0000312|EMBL:BAF23295.1},
GN LOC_Os08g15460 {ECO:0000305};
GN ORFNames=OJ1112_D12.8, OJ1575_B01.20,
GN OsJ_26630 {ECO:0000312|EMBL:EAZ42069.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: The central subunit of the protein translocation channel
CC SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two
CC domains form a lateral gate at the front which open onto the bilayer
CC between TMs 2 and 7, and are clamped together by SecE at the back. The
CC channel is closed by both a pore ring composed of hydrophobic SecY
CC resides and a short helix (helix 2A) on the extracellular side of the
CC membrane which forms a plug (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the plastid Sec protein translocase complex,
CC which is composed of at least SECY and SECE. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SecY/SEC61-alpha family. {ECO:0000305}.
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DR EMBL; AP004013; BAC98517.1; -; Genomic_DNA.
DR EMBL; AP004155; BAC98535.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23295.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT04569.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ42069.1; -; Genomic_DNA.
DR EMBL; AK072225; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650895.1; XM_015795409.1.
DR AlphaFoldDB; Q6ZG25; -.
DR STRING; 4530.OS08T0254500-02; -.
DR PaxDb; Q6ZG25; -.
DR PRIDE; Q6ZG25; -.
DR EnsemblPlants; Os08t0254500-01; Os08t0254500-01; Os08g0254500.
DR EnsemblPlants; Os08t0254500-02; Os08t0254500-02; Os08g0254500.
DR GeneID; 4345097; -.
DR Gramene; Os08t0254500-01; Os08t0254500-01; Os08g0254500.
DR Gramene; Os08t0254500-02; Os08t0254500-02; Os08g0254500.
DR KEGG; osa:4345097; -.
DR eggNOG; ENOG502QSNV; Eukaryota.
DR HOGENOM; CLU_030313_0_0_1; -.
DR InParanoid; Q6ZG25; -.
DR OMA; FAMWLGE; -.
DR OrthoDB; 912533at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q6ZG25; baseline and differential.
DR Genevisible; Q6ZG25; OS.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005048; F:signal sequence binding; IBA:GO_Central.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR Gene3D; 1.10.3370.10; -; 1.
DR HAMAP; MF_01465; SecY; 1.
DR InterPro; IPR026593; SecY.
DR InterPro; IPR002208; SecY/SEC61-alpha.
DR InterPro; IPR030659; SecY_CS.
DR InterPro; IPR023201; SecY_dom_sf.
DR PANTHER; PTHR10906; PTHR10906; 1.
DR Pfam; PF00344; SecY; 1.
DR SUPFAM; SSF103491; SSF103491; 1.
DR TIGRFAMs; TIGR00967; 3a0501s007; 1.
DR PROSITE; PS00755; SECY_1; 1.
DR PROSITE; PS00756; SECY_2; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Protein transport; Reference proteome;
KW Thylakoid; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 69..556
FT /note="Preprotein translocase subunit SECY, chloroplastic"
FT /id="PRO_0000247496"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..30
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 486
FT /note="S -> Y (in Ref. 5; AK072225)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 556 AA; 59530 MW; 3A6497F1E40FE6AE CRC64;
MAAMATPQQC WLPTRARAPP PPPPRAPSSA SPVSGAPASL RFRGRRAASA SASAARRRRG
ASLPCSPRCA LETAGPGFDP LGLYNDGPSR NDTQSPLSNF FGVLSPVFGS SSGGRKEKSY
GRGAAAAIED SSIDIGDFFK GPLPGKFLKL LGYLALSRLG IYIPLGGVNR DAFAGNLDQN
SLLGTLDSFS GGGIGRLGIC SLGIVPFINA QIVFQLLAQL YPKLQDLQKK EGEAGRKKVL
QYTRYASVGF AIVQAIGQVL FLRPYVNDFS TEWVLTSVTL LTLGSVFTTF IGERISDLKL
GNGTSLLIFT SIISYLPASF GRTVAQAFQD GNYVGLLTII LSFLFLVLGI VYVQEAERKI
PLNYASRYSS RSGGLQRSAY LPFKVNSSGV MPIIFSTSSL ALPGTLARFT GLEFLKKAAI
SLNPGGALYI PTNVLLIAFF NYYYTFLQLD PDDLSEQLKR QGASIPLVRP GKSTAAFIKT
VLSNISVLGS AFLAVLAAGP SVVEQITHLT AFRGFAGTSV LILVGCATDT ARKVQAEIIS
QKYKNIEFYD VNRFDQ
