ID   SECY_SPIOL              Reviewed;         545 AA.
AC   P93690;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Preprotein translocase subunit SECY, chloroplastic;
DE   AltName: Full=CpSecY;
DE   Flags: Precursor;
GN   Name=SECY;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Monatol; TISSUE=Leaf;
RA   Berghoefer J., Kloesgen R.B.;
RT   "Isolation and characterization of a cDNA encoding the SecY protein from
RT   spinach chloroplasts.";
RL   (er) Plant Gene Register PGR96-090(1996).
CC   -!- FUNCTION: The central subunit of the protein translocation channel
CC       SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two
CC       domains form a lateral gate at the front which open onto the bilayer
CC       between TMs 2 and 7, and are clamped together by SecE at the back. The
CC       channel is closed by both a pore ring composed of hydrophobic SecY
CC       resides and a short helix (helix 2A) on the extracellular side of the
CC       membrane which forms a plug (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the plastid Sec protein translocase complex,
CC       which is composed of at least SECY and SECE. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SecY/SEC61-alpha family. {ECO:0000305}.
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DR   EMBL; Z54351; CAA91162.1; -; mRNA.
DR   PIR; T09195; T09195.
DR   AlphaFoldDB; P93690; -.
DR   SMR; P93690; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3370.10; -; 1.
DR   HAMAP; MF_01465; SecY; 1.
DR   InterPro; IPR026593; SecY.
DR   InterPro; IPR002208; SecY/SEC61-alpha.
DR   InterPro; IPR030659; SecY_CS.
DR   InterPro; IPR023201; SecY_dom_sf.
DR   PANTHER; PTHR10906; PTHR10906; 1.
DR   Pfam; PF00344; SecY; 1.
DR   SUPFAM; SSF103491; SSF103491; 1.
DR   TIGRFAMs; TIGR00967; 3a0501s007; 1.
DR   PROSITE; PS00755; SECY_1; 1.
DR   PROSITE; PS00756; SECY_2; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Membrane; Plastid; Protein transport; Thylakoid;
KW   Transit peptide; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..545
FT                   /note="Preprotein translocase subunit SECY, chloroplastic"
FT                   /id="PRO_0000031998"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        477..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        500..520
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   545 AA;  58501 MW;  26ED3D90E0E142B4 CRC64;
     MLVTFTEAAA TASTSSLISF SLSSPPKFHH KSRIYGCKAT FGLRTKPTSW IAAASLSSST
     TTTSSCGSSV FDPLGIEQDN SWGVASAWDG VLKFVSQTFE SSSGTRKDRS SSARGVAAAI
     EDSSIDFGDF FKGPLPGKFL TLLGLLALSR LGIYVPLGGV NREAFVGNLD QNSFISTLDS
     FSGGGIGRLG ICSLGIVPFI NAQIVFQLLS QVYPKLQDLQ KKEGEAGRKK IKQYTQYASV
     GFALVQAIGQ VLFLRPYVND YSTEWVLSSV ILLTLGSVFT TYLGERISDL KLGNGTSLLI
     FTNIISYLPA SFGRTVAEAY QEGNYTGLAI IVVSFVSLVF GIVYVQEAER KIPMNYASRY
     SGKSGGLQKS AYLPFKVNSA GVMPIIFSTS SLSLPATLAR FTGLDILKKA AVALTPGGSF
     YLPTNILLIA FFNYYYTFLQ LDPDDVSEQL KRQGASIPLV RPGKSTAAYI KTVLSRISVL
     GSGFLAILAA GPAVVEQATH LTAFRGFAGT SVLILVGCAT DTARKVQAEL ISQKYKNIEF
     YDLEK