SED5_YEAST
ID SED5_YEAST Reviewed; 340 AA.
AC Q01590; D6VY28;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Integral membrane protein SED5;
GN Name=SED5; OrderedLocusNames=YLR026C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1400588; DOI=10.1083/jcb.119.3.513;
RA Hardwick K.G., Pelham H.R.B.;
RT "SED5 encodes a 39-kD integral membrane protein required for vesicular
RT transport between the ER and the Golgi complex.";
RL J. Cell Biol. 119:513-521(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP INTERACTION WITH GOS1.
RX PubMed=9755865; DOI=10.1016/s0014-5793(98)01044-8;
RA McNew J.A., Coe J.G.S., Sogaard M., Zemelman B.V., Wimmer C., Hong W.,
RA Soellner T.H.;
RT "Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi
RT transport.";
RL FEBS Lett. 435:89-95(1998).
RN [5]
RP INTERACTION WITH SEC24, AND MUTAGENESIS OF ASN-206.
RX PubMed=12941276; DOI=10.1016/s0092-8674(03)00608-1;
RA Mossessova E., Bickford L.C., Goldberg J.;
RT "SNARE selectivity of the COPII coat.";
RL Cell 114:483-495(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-45 IN COMPLEX WITH SLY1.
RX PubMed=12426383; DOI=10.1093/emboj/cdf608;
RA Bracher A., Weissenhorn W.;
RT "Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p.";
RL EMBO J. 21:6114-6124(2002).
CC -!- FUNCTION: Required for vesicular transport between the endoplasmic
CC reticulum and the Golgi complex. Acts as a target organelle soluble NSF
CC attachment protein receptor (t-SNARE).
CC -!- SUBUNIT: Interacts with SLY1, STF1, SFB3 and GOS1.
CC {ECO:0000269|PubMed:12426383, ECO:0000269|PubMed:12941276,
CC ECO:0000269|PubMed:9755865}.
CC -!- INTERACTION:
CC Q01590; P53271: COG2; NbExp=2; IntAct=EBI-16930, EBI-16614;
CC Q01590; P40094: COG3; NbExp=4; IntAct=EBI-16930, EBI-16605;
CC Q01590; P38736: GOS1; NbExp=6; IntAct=EBI-16930, EBI-24365;
CC Q01590; P22214: SEC22; NbExp=13; IntAct=EBI-16930, EBI-16577;
CC Q01590; P43682: SFT1; NbExp=2; IntAct=EBI-16930, EBI-17040;
CC Q01590; P22213: SLY1; NbExp=5; IntAct=EBI-16930, EBI-17387;
CC Q01590; Q04338: VTI1; NbExp=2; IntAct=EBI-16930, EBI-20519;
CC Q01590; P01123: YPT1; NbExp=3; IntAct=EBI-16930, EBI-29496;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type IV
CC membrane protein {ECO:0000305}. Golgi apparatus membrane {ECO:0000305};
CC Single-pass type IV membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 300 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X66980; CAA47390.1; -; Genomic_DNA.
DR EMBL; Z73198; CAA97549.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09344.1; -; Genomic_DNA.
DR PIR; A44019; A44019.
DR RefSeq; NP_013126.1; NM_001181913.1.
DR PDB; 1MQS; X-ray; 3.00 A; B=1-45.
DR PDB; 1PD0; X-ray; 2.60 A; B=201-210.
DR PDBsum; 1MQS; -.
DR PDBsum; 1PD0; -.
DR AlphaFoldDB; Q01590; -.
DR BMRB; Q01590; -.
DR SMR; Q01590; -.
DR BioGRID; 31300; 495.
DR ComplexPortal; CPX-1854; Golgi SNARE complex SED5-BOS1-BET1-SEC22.
DR ComplexPortal; CPX-1855; Golgi SNARE complex SED5-GOS1-SFT1-YKT6.
DR DIP; DIP-2489N; -.
DR IntAct; Q01590; 18.
DR MINT; Q01590; -.
DR STRING; 4932.YLR026C; -.
DR TCDB; 8.A.91.1.10; the syntaxin (syntaxin) family.
DR iPTMnet; Q01590; -.
DR MaxQB; Q01590; -.
DR PaxDb; Q01590; -.
DR PRIDE; Q01590; -.
DR EnsemblFungi; YLR026C_mRNA; YLR026C; YLR026C.
DR GeneID; 850713; -.
DR KEGG; sce:YLR026C; -.
DR SGD; S000004016; SED5.
DR VEuPathDB; FungiDB:YLR026C; -.
DR eggNOG; KOG0812; Eukaryota.
DR GeneTree; ENSGT01000000214440; -.
DR HOGENOM; CLU_044998_0_1_1; -.
DR InParanoid; Q01590; -.
DR OMA; RGITGTM; -.
DR BioCyc; YEAST:G3O-32187-MON; -.
DR Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR Reactome; R-SCE-5694530; Cargo concentration in the ER.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811438; Intra-Golgi traffic.
DR Reactome; R-SCE-9013106; RHOC GTPase cycle.
DR Reactome; R-SCE-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR EvolutionaryTrace; Q01590; -.
DR PRO; PR:Q01590; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q01590; protein.
DR GO; GO:0005801; C:cis-Golgi network; IDA:SGD.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IC:ComplexPortal.
DR GO; GO:1990674; C:Golgi cis cisterna membrane; IC:ComplexPortal.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IDA:SGD.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR GO; GO:0005484; F:SNAP receptor activity; IDA:SGD.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:ComplexPortal.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IDA:ComplexPortal.
DR GO; GO:0090083; P:regulation of inclusion body assembly; IDA:SGD.
DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IDA:SGD.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:ComplexPortal.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR045242; Syntaxin.
DR InterPro; IPR021538; Syntaxin-5_N.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19957; PTHR19957; 1.
DR Pfam; PF05739; SNARE; 1.
DR Pfam; PF11416; Syntaxin-5_N; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..340
FT /note="Integral membrane protein SED5"
FT /id="PRO_0000210273"
FT TOPO_DOM 1..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT DOMAIN 249..311
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT REGION 31..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 146..173
FT /evidence="ECO:0000255"
FT COMPBIAS 183..231
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 206
FT /note="N->A: Loss of interaction with SEC23/SEC24 complex."
FT /evidence="ECO:0000269|PubMed:12941276"
FT HELIX 1..4
FT /evidence="ECO:0007829|PDB:1MQS"
FT HELIX 7..20
FT /evidence="ECO:0007829|PDB:1MQS"
SQ SEQUENCE 340 AA; 38807 MW; B3192B57269A0B83 CRC64;
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS VSEFQKKASG
IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK RKIYAIEQSL VQLSQLKKTD
VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM KNISGSFKDV LEERQRLEMA NKDRWQKLTT
DTGHAPADDQ TQSNHAADLT TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML
MEEGQLSNNV YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN
