SEGN_DANRE
ID SEGN_DANRE Reviewed; 272 AA.
AC Q5XJX1;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Secretagogin;
GN Name=scgn; ORFNames=zgc:100843;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
RG Center for eukaryotic structural genomics (CESG);
RT "X-ray structure of an EF-hand protein from Danio rerio DR.36843.";
RL Submitted (NOV-2005) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; BC083168; AAH83168.1; -; mRNA.
DR RefSeq; NP_001005776.1; NM_001005776.1.
DR PDB; 2BE4; X-ray; 2.10 A; A=2-272.
DR PDB; 2Q4U; X-ray; 2.10 A; A=2-272.
DR PDB; 6JLH; X-ray; 2.37 A; A/C=7-267.
DR PDBsum; 2BE4; -.
DR PDBsum; 2Q4U; -.
DR PDBsum; 6JLH; -.
DR AlphaFoldDB; Q5XJX1; -.
DR SMR; Q5XJX1; -.
DR STRING; 7955.ENSDARP00000076090; -.
DR PaxDb; Q5XJX1; -.
DR Ensembl; ENSDART00000081649; ENSDARP00000076090; ENSDARG00000058732.
DR Ensembl; ENSDART00000181959; ENSDARP00000155441; ENSDARG00000115991.
DR GeneID; 573010; -.
DR KEGG; dre:573010; -.
DR CTD; 10590; -.
DR ZFIN; ZDB-GENE-041010-82; scgn.
DR eggNOG; KOG0027; Eukaryota.
DR GeneTree; ENSGT00950000183108; -.
DR HOGENOM; CLU_054826_1_1_1; -.
DR InParanoid; Q5XJX1; -.
DR OMA; KMDACSS; -.
DR OrthoDB; 979297at2759; -.
DR PhylomeDB; Q5XJX1; -.
DR TreeFam; TF325083; -.
DR EvolutionaryTrace; Q5XJX1; -.
DR PRO; PR:Q5XJX1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000058732; Expressed in head kidney and 16 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IMP:ZFIN.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:1900271; P:regulation of long-term synaptic potentiation; IBA:GO_Central.
DR GO; GO:0099509; P:regulation of presynaptic cytosolic calcium ion concentration; IBA:GO_Central.
DR CDD; cd16178; EFh_HEF_SCGN; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR035798; EFh_SCGN.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 5.
DR SUPFAM; SSF47473; SSF47473; 2.
DR PROSITE; PS00018; EF_HAND_1; 5.
DR PROSITE; PS50222; EF_HAND_2; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cytoplasm; Metal-binding; Reference proteome;
KW Repeat.
FT CHAIN 1..272
FT /note="Secretagogin"
FT /id="PRO_0000330627"
FT DOMAIN 8..43
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 53..89
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 101..136
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 145..180
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 193..228
FT /note="EF-hand 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 237..272
FT /note="EF-hand 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 32
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 114
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 116
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 118
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 125
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 158
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 160
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 162
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 164
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 169
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 206
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 208
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 210
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 217
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 250
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 252
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 254
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 256
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 261
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT HELIX 10..20
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 33..44
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 52..62
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 65..68
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 72..75
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 76..83
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 86..97
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 103..113
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 119..122
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 126..136
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 143..157
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 167..170
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 171..173
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 180..183
FT /evidence="ECO:0007829|PDB:6JLH"
FT HELIX 188..205
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 216..229
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 235..249
FT /evidence="ECO:0007829|PDB:2BE4"
FT STRAND 251..253
FT /evidence="ECO:0007829|PDB:2Q4U"
FT STRAND 256..258
FT /evidence="ECO:0007829|PDB:2BE4"
FT HELIX 259..265
FT /evidence="ECO:0007829|PDB:2BE4"
SQ SEQUENCE 272 AA; 31578 MW; CD2AD9A80017CAE0 CRC64;
MDSAFANLDA AGFLQIWQHF DADDNGYIEG KELDDFFRHM LKKLQPKDKI TDERVQQIKK
SFMSAYDATF DGRLQIEELA NMILPQEENF LLIFRREAPL DNSVEFMKIW RKYDADSSGY
ISAAELKNFL KDLFLQHKKK IPPNKLDEYT DAMMKIFDKN KDGRLDLNDL ARILALQENF
LLQFKMDASS QVERKRDFEK IFAHYDVSRT GALEGPEVDG FVKDMMELVR PSISGGDLDK
FRECLLTHCD MNKDGKIQKS ELALCLGLKH KP
