SEH1_ARATH
ID SEH1_ARATH Reviewed; 326 AA.
AC Q93VR9; Q8LB12; Q9C5X7; Q9C7V9;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Protein SEH1 {ECO:0000303|PubMed:21189294};
GN Name=SEH1 {ECO:0000303|PubMed:21189294}; Synonyms=SEH1H;
GN OrderedLocusNames=At1g64350 {ECO:0000312|Araport:AT1G64350};
GN ORFNames=F15H21.2 {ECO:0000312|EMBL:AAG51710.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL15222.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Lee S.H., Jin J.B., Hwang I.;
RT "Arabidopsis thaliana seh1 homolog (seh1h) mRNA.";
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=22288649; DOI=10.1111/j.1365-313x.2012.04928.x;
RA Wiermer M., Cheng Y.T., Imkampe J., Li M., Wang D., Lipka V., Li X.;
RT "Putative members of the Arabidopsis Nup107-160 nuclear pore sub-complex
RT contribute to pathogen defense.";
RL Plant J. 70:796-808(2012).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22902705; DOI=10.4161/psb.21426;
RA Roth C., Wiermer M.;
RT "Nucleoporins Nup160 and Seh1 are required for disease resistance in
RT Arabidopsis.";
RL Plant Signal. Behav. 7:1212-1214(2012).
CC -!- FUNCTION: Required for proper export of mRNAs from the nucleus to the
CC cytoplasm. {ECO:0000269|PubMed:22288649}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. {ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:21189294,
CC ECO:0000269|PubMed:22902705}. Nucleus {ECO:0000269|PubMed:22288649,
CC ECO:0000269|PubMed:22902705}. Cytoplasm {ECO:0000269|PubMed:22288649,
CC ECO:0000269|PubMed:22902705}. Nucleus, nuclear pore complex
CC {ECO:0000305|PubMed:21189294}. Note=Part of the cellular SEH1 pool is
CC not permanently associated with the Nup107-160 complex.
CC {ECO:0000305|PubMed:22902705}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, but enhanced susceptibility
CC to virulent Pseudomonas bacteria and oomycete pathogens.
CC {ECO:0000269|PubMed:22288649}.
CC -!- SIMILARITY: Belongs to the WD repeat SEC13 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51710.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF325901; AAG59882.1; -; mRNA.
DR EMBL; AC066689; AAG51710.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34229.1; -; Genomic_DNA.
DR EMBL; AF370222; AAK44037.1; -; mRNA.
DR EMBL; AY059116; AAL15222.1; -; mRNA.
DR EMBL; AY087489; AAM65032.1; -; mRNA.
DR PIR; E96667; E96667.
DR RefSeq; NP_564830.1; NM_105109.4.
DR AlphaFoldDB; Q93VR9; -.
DR SMR; Q93VR9; -.
DR BioGRID; 27962; 4.
DR STRING; 3702.AT1G64350.1; -.
DR PaxDb; Q93VR9; -.
DR PRIDE; Q93VR9; -.
DR ProteomicsDB; 232671; -.
DR EnsemblPlants; AT1G64350.1; AT1G64350.1; AT1G64350.
DR GeneID; 842741; -.
DR Gramene; AT1G64350.1; AT1G64350.1; AT1G64350.
DR KEGG; ath:AT1G64350; -.
DR Araport; AT1G64350; -.
DR TAIR; locus:2014210; AT1G64350.
DR eggNOG; KOG2445; Eukaryota.
DR HOGENOM; CLU_032441_1_2_1; -.
DR InParanoid; Q93VR9; -.
DR OMA; NAPTRRW; -.
DR OrthoDB; 944756at2759; -.
DR PhylomeDB; Q93VR9; -.
DR PRO; PR:Q93VR9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93VR9; baseline and differential.
DR Genevisible; Q93VR9; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0031080; C:nuclear pore outer ring; IBA:GO_Central.
DR GO; GO:0035859; C:Seh1-associated complex; IBA:GO_Central.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0034198; P:cellular response to amino acid starvation; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR037597; Nucleoporin_Seh1.
DR InterPro; IPR037363; Sec13/Seh1_fam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR11024; PTHR11024; 1.
DR PANTHER; PTHR11024:SF3; PTHR11024:SF3; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; mRNA transport; Nuclear pore complex; Nucleus;
KW Protein transport; Reference proteome; Repeat; Translocation; Transport;
KW WD repeat.
FT CHAIN 1..326
FT /note="Protein SEH1"
FT /id="PRO_0000431094"
FT REPEAT 7..46
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 54..95
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 105..146
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 224..266
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 278..317
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT CONFLICT 68
FT /note="E -> V (in Ref. 5; AAM65032)"
FT CONFLICT 190
FT /note="A -> T (in Ref. 1; AAG59882)"
FT CONFLICT 203
FT /note="I -> N (in Ref. 1; AAG59882)"
SQ SEQUENCE 326 AA; 35696 MW; 8FB73B88E60DD616 CRC64;
MAKSMATLDS GTTCSSWNQS GDRLAAGSLN GKLSIYESST SSSSTFSCTS KVRVSESSIV
KIVWLPSEYG DAVACVCEDG SLSIWEELSE DAHGLEWKLC KSMKNKSSQV LDVQFGVSRK
SLKMVAAYSD GYLRVFELLN PLELKNWQLQ AEFQNVIDSL STLGKPSSLS ASVSWNPMKG
EEQEPSFVLA FNSDSPHLNS SKIWEFDEAH NRWLAVAELA LPEDKGDPVY ALSWAPNIGR
PYEVVAVATH KGIGIWHVGL APDLEGRLPV KKVSSLSGHQ GEVWQMEWDM SGMTLASTGS
DGMVKLWQSN LNGEWHEQAT LEPVPS
