ID   SEI2_ARATH              Reviewed;         526 AA.
AC   F4I340; Q9FXF3;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Seipin-2 {ECO:0000303|PubMed:26362606};
DE            Short=AtSEIPIN2 {ECO:0000303|PubMed:26362606};
GN   Name=SEI2 {ECO:0000305};
GN   OrderedLocusNames=At1g29760 {ECO:0000312|Araport:AT1G29760};
GN   ORFNames=F1N18.1 {ECO:0000312|EMBL:AAG10618.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=26362606; DOI=10.1105/tpc.15.00588;
RA   Cai Y., Goodman J.M., Pyc M., Mullen R.T., Dyer J.M., Chapman K.D.;
RT   "Arabidopsis SEIPIN proteins modulate triacylglycerol accumulation and
RT   influence lipid droplet proliferation.";
RL   Plant Cell 27:2616-2636(2015).
CC   -!- FUNCTION: Involved in lipid metabolism and lipid droplet (LD)
CC       morphology, number, and size. Supports the formation of small-sized LDs
CC       and modulates triacylglycerol accumulation. Induces probably a
CC       reorganization of the endoplasmic reticulum into LD-forming domains.
CC       {ECO:0000269|PubMed:26362606}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:26362606}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Localized to the lipid droplet-forming sites.
CC       {ECO:0000269|PubMed:26362606}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds, seedlings, leaves, stems and
CC       roots. Not detected in flowers. {ECO:0000269|PubMed:26362606}.
CC   -!- SIMILARITY: Belongs to the seipin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG10618.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC008030; AAG10618.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE31126.1; -; Genomic_DNA.
DR   PIR; B86421; B86421.
DR   RefSeq; NP_174269.1; NM_102716.4.
DR   AlphaFoldDB; F4I340; -.
DR   STRING; 3702.AT1G29760.1; -.
DR   PaxDb; F4I340; -.
DR   PRIDE; F4I340; -.
DR   ProteomicsDB; 232971; -.
DR   EnsemblPlants; AT1G29760.1; AT1G29760.1; AT1G29760.
DR   GeneID; 839854; -.
DR   Gramene; AT1G29760.1; AT1G29760.1; AT1G29760.
DR   KEGG; ath:AT1G29760; -.
DR   Araport; AT1G29760; -.
DR   TAIR; locus:2019347; AT1G29760.
DR   eggNOG; KOG4200; Eukaryota.
DR   HOGENOM; CLU_035656_1_1_1; -.
DR   InParanoid; F4I340; -.
DR   OMA; WNVAFRC; -.
DR   OrthoDB; 782411at2759; -.
DR   PRO; PR:F4I340; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I340; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0140042; P:lipid droplet formation; IGI:TAIR.
DR   GO; GO:0034389; P:lipid droplet organization; IMP:UniProtKB.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   GO; GO:0009846; P:pollen germination; IGI:TAIR.
DR   GO; GO:0080155; P:regulation of double fertilization forming a zygote and endosperm; IGI:TAIR.
DR   GO; GO:0010162; P:seed dormancy process; IGI:TAIR.
DR   InterPro; IPR009617; Seipin.
DR   PANTHER; PTHR21212; PTHR21212; 1.
DR   Pfam; PF06775; Seipin; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Lipid metabolism; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..526
FT                   /note="Seipin-2"
FT                   /id="PRO_0000434816"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        483..503
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          33..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   526 AA;  59677 MW;  677A8A63B741B55B CRC64;
     MDSESESESN PSTTDEFDRF LDAPDEFYYD CLPIRSNSHQ PSSLLRRRKS AHRRDLISSD
     IETEPSSSSD GFDVGEKSSY VEKNAELRGD IDTSDVIEST KDSIDLSSEK ENDLDVISSS
     GNDMDVIDSG RNRVDPFQEE STVTTVSSDD QGDDDYAGSV PQFREPPNST EWSLLGFLVG
     LVIKAIEFQV SFMTSLLTFP PWLLRNCFLF FFDPFSTIRF GRRFLMARVA GISDMIFGYM
     NPFRLKDTKQ MLSIVCKFGW GMFWAVYVGI VLFGLLVSSL MIGGYVINRI ADKPFEVKET
     LNFDYTKNSP EAYVPISSCA GVECEGSCKE SNEMSKIRGL RVIPRDQKLD IILSMTLPES
     AYNKNLGMFQ VRVDFLSVDG QTIASIRRPC MLRFRSEPIR LVQTFFKVVP LVTGYVSEIQ
     TLSLKLKGFV EKDIPTACLK IIIEQRAEFR PGAGIPELYD ASLSVESGLP FFRKIIWKWR
     KTLFVWISMS LFITELLFTL VCCRPLIIPR TQPRDRSPSN PTGVWR