ID   BGAL_LACLA              Reviewed;         998 AA.
AC   Q48727; P71448; Q9CE65;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 3.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Beta-galactosidase;
DE            EC=3.2.1.23;
DE   AltName: Full=Lactase;
GN   Name=lacZ; OrderedLocusNames=LL1980; ORFNames=L0025;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 7962;
RA   Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.;
RT   "Cloning and nucleotide sequence of the beta-galactosidase gene from
RT   Lactococcus lactis ssp. lactis ATCC7962.";
RL   Biotechnol. Lett. 19:179-183(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 7962;
RX   PubMed=8988372; DOI=10.3109/10425179609047572;
RA   Griffin H.G., Maccormick C.A., Gasson M.J.;
RT   "Cloning, DNA sequence, and regulation of expression of a gene encoding
RT   beta-galactosidase from Lactococcus lactis.";
RL   DNA Seq. 6:337-346(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK06078.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA56341.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U60828; AAD11504.1; -; Genomic_DNA.
DR   EMBL; X80037; CAA56341.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE005176; AAK06078.1; ALT_INIT; Genomic_DNA.
DR   PIR; D86872; D86872.
DR   RefSeq; NP_268137.2; NC_002662.1.
DR   RefSeq; WP_010906246.1; NC_002662.1.
DR   AlphaFoldDB; Q48727; -.
DR   SMR; Q48727; -.
DR   STRING; 272623.L0025; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   PaxDb; Q48727; -.
DR   EnsemblBacteria; AAK06078; AAK06078; L0025.
DR   KEGG; lla:L0025; -.
DR   PATRIC; fig|272623.7.peg.2132; -.
DR   eggNOG; COG3250; Bacteria.
DR   HOGENOM; CLU_002346_0_2_9; -.
DR   OMA; SNWQLQG; -.
DR   SABIO-RK; Q48727; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IEA:UniProt.
DR   Gene3D; 2.60.40.10; -; 2.
DR   Gene3D; 2.70.98.10; -; 1.
DR   InterPro; IPR004199; B-gal_small/dom_5.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR023232; Glyco_hydro_2_AS.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR023230; Glyco_hydro_2_CS.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032312; LacZ_4.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF16353; DUF4981; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   SMART; SM01038; Bgal_small_N; 1.
DR   SUPFAM; SSF49303; SSF49303; 2.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF74650; SSF74650; 1.
DR   PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..998
FT                   /note="Beta-galactosidase"
FT                   /id="PRO_0000057669"
FT   ACT_SITE        431
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        508
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   VARIANT         34
FT                   /note="L -> F (in strain: ATCC 7962)"
FT   VARIANT         182
FT                   /note="V -> G (in strain: ATCC 7962)"
FT   VARIANT         237
FT                   /note="I -> M (in strain: ATCC 7962)"
FT   VARIANT         629
FT                   /note="I -> T (in strain: ATCC 7962)"
FT   VARIANT         677
FT                   /note="C -> Y (in strain: ATCC 7962)"
FT   VARIANT         702
FT                   /note="R -> K (in strain: ATCC 7962)"
FT   VARIANT         712
FT                   /note="F -> L (in strain: ATCC 7962)"
FT   VARIANT         784..785
FT                   /note="GI -> AV (in strain: ATCC 7962)"
FT   VARIANT         796
FT                   /note="T -> N (in strain: ATCC 7962)"
FT   VARIANT         919
FT                   /note="D -> G (in strain: ATCC 7962)"
SQ   SEQUENCE   998 AA;  115440 MW;  8989FD4287ADDA89 CRC64;
     MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS RISDVPKNWL
     ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP YVPEANPVGA YSRYFDITKE
     WLESGHVHLT FEGVGSAFHF WLNGEYGGYS EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS
     KVTYFEDQDM WRMSGIFRSV NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA
     CLEFKLYDDE QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI
     GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK EHNFNAVRCS
     HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP TYLPLMSERV TRMVMRDRNH
     PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS SRPVHYEGGD DASRGATDAT DIICPMYARV
     DSPSINAPYS LKTWMGVAGE NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD
     WVDQGLLKDG NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT
     PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM RIDLSELPID
     GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT DRIDSNEEIT LFEDEELLTV
     RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE
     RWKGIGFYEL KTLLKTMIIQ ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV
     DFKRNIEFPE PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP
     YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL LEEEAGTWLN
     IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT