ID   BGAL_SULAC              Reviewed;         491 AA.
AC   P14288; Q4J7S6;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 2.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=Beta-galactosidase;
DE            Short=Lactase;
DE            EC=3.2.1.23;
GN   Name=bgaS; OrderedLocusNames=Saci_1849;
OS   Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS   15157 / NCIMB 11770).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=330779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2508066; DOI=10.1093/nar/17.19.7980;
RA   Little S., Cartwright P., Campbell C., Prenneta A., McChesney J.,
RA   Mountain A., Robinson M.;
RT   "Nucleotide sequence of a thermostable beta-galactosidase from Sulfolobus
RT   solfataricus.";
RL   Nucleic Acids Res. 17:7980-7980(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX   PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA   Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA   Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT   "The genome of Sulfolobus acidocaldarius, a model organism of the
RT   Crenarchaeota.";
RL   J. Bacteriol. 187:4992-4999(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Thermostable.;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to originate from S.solfataricus strain
CC       P1, but the culture was contaminated with S.acidocaldarius.
CC       {ECO:0000305|PubMed:2508066}.
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DR   EMBL; X15950; CAA34074.1; -; Genomic_DNA.
DR   EMBL; CP000077; AAY81155.1; -; Genomic_DNA.
DR   RefSeq; WP_011278657.1; NC_007181.1.
DR   AlphaFoldDB; P14288; -.
DR   SMR; P14288; -.
DR   STRING; 330779.Saci_1849; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   EnsemblBacteria; AAY81155; AAY81155; Saci_1849.
DR   GeneID; 3474772; -.
DR   KEGG; sai:Saci_1849; -.
DR   PATRIC; fig|330779.12.peg.1796; -.
DR   eggNOG; arCOG05412; Archaea.
DR   HOGENOM; CLU_001859_1_3_2; -.
DR   OMA; VEACDRK; -.
DR   BRENDA; 3.2.1.B34; 6160.
DR   Proteomes; UP000001018; Chromosome.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR018120; Glyco_hydro_1_AS.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 2.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   1: Evidence at protein level;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..491
FT                   /note="Beta-galactosidase"
FT                   /id="PRO_0000063866"
FT   ACT_SITE        209
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        389
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10055"
FT   CONFLICT        135
FT                   /note="Q -> H (in Ref. 1; CAA34074)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   491 AA;  57143 MW;  A7FF7F4DEEC1C189 CRC64;
     MLSFPKGFKF GWSQSGFQSE MGTPGSEDPN SDWHVWVHDR ENIVSQVVSG DLPENGPGYW
     GNYKRFHDEA EKIGLNAVRI NVEWSRIFPR PLPKPEMQTG TDKENSPVIS VDLNESKLRE
     MDNYANHEAL SHYRQILEDL RNRGFHIVLN MYHWTLPIWL HDPIRVRRGD FTGPTGWLNS
     RTVYEFARFS AYVAWKLDDL ASEYATMNEP NVVWGAGYAF PRAGFPPNYL SFRLSEIAKW
     NIIQAHARAY DAIKSVSKKS VGIIYANTSY YPLRPQDNEA VEIAERLNRW SFFDSIIKGE
     ITSEGQNVRE DLRNRLDWIG VNYYTRTVVT KAESGYLTLP GYGDRCERNS LSLANLPTSD
     FGWEFFPEGL YDVLLKYWNR YGLPLYVMEN GIADDADYQR PYYLVSHIYQ VHRALNEGVD
     VRGYLHWSLA DNYEWSSGFS MRFGLLKVDY LTKRLYWRPS ALVYREITRS NGIPEELEHL
     NRVPPIKPLR H