BGAL_THEMA
ID BGAL_THEMA Reviewed; 1084 AA.
AC Q56307; O33834;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Beta-galactosidase;
DE Short=Beta-gal;
DE EC=3.2.1.23;
DE AltName: Full=Lactase;
GN Name=lacZ; OrderedLocusNames=TM_1193;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=8088532; DOI=10.1016/0378-1119(94)90046-9;
RA Moore J.B., Markiewicz P., Miller J.H.;
RT "Identification and sequencing of the Thermotoga maritima lacZ gene, part
RT of a divergently transcribed operon.";
RL Gene 147:101-106(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=9741105; DOI=10.1016/s0723-2020(98)80002-7;
RA Liebl W., Wagner B., Schellhase J.;
RT "Properties of an alpha-galactosidase, and structure of its gene galA,
RT within an alpha- and beta-galactoside utilization gene cluster of the
RT hyperthermophilic bacterium Thermotoga maritima.";
RL Syst. Appl. Microbiol. 21:1-11(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD36268.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U08186; AAA50597.1; -; Genomic_DNA.
DR EMBL; AE000512; AAD36268.1; ALT_INIT; Genomic_DNA.
DR EMBL; AJ001072; CAA04513.1; -; Genomic_DNA.
DR PIR; F72283; F72283.
DR RefSeq; NP_228998.1; NC_000853.1.
DR RefSeq; WP_004080135.1; NZ_CP011107.1.
DR PDB; 6S6Z; EM; 2.10 A; A/B/C/D/E/F/G/H=2-1084.
DR PDB; 6SD0; X-ray; 3.70 A; A/B/C/D=1-1084.
DR PDBsum; 6S6Z; -.
DR PDBsum; 6SD0; -.
DR AlphaFoldDB; Q56307; -.
DR SMR; Q56307; -.
DR STRING; 243274.THEMA_08365; -.
DR CAZy; GH2; Glycoside Hydrolase Family 2.
DR PRIDE; Q56307; -.
DR EnsemblBacteria; AAD36268; AAD36268; TM_1193.
DR KEGG; tma:TM1193; -.
DR PATRIC; fig|243274.5.peg.1211; -.
DR eggNOG; COG3250; Bacteria.
DR InParanoid; Q56307; -.
DR OMA; SNWQLQG; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005990; P:lactose catabolic process; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.70.98.10; -; 1.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF16353; DUF4981; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF49303; SSF49303; 2.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosidase; Hydrolase; Reference proteome.
FT CHAIN 1..1084
FT /note="Beta-galactosidase"
FT /id="PRO_0000057678"
FT ACT_SITE 441
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 507
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT CONFLICT 152..175
FT /note="SKDSCTPAEFRLTDVLRPGKNLIT -> RQRQLHARRIQTHRCSKTREESDH
FT (in Ref. 1; AAA50597)"
FT /evidence="ECO:0000305"
FT CONFLICT 1028..1084
FT /note="SKEKQVVLFVDGNEYSVRRVVIPPFKKEELVFKVEGLKKGEHLIHTNLNTRK
FT TIYVR -> RQGKTGGSLC (in Ref. 1)"
FT /evidence="ECO:0000305"
FT HELIX 4..6
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 28..30
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 37..41
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 44..53
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 71..77
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 89..94
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 111..120
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 122..126
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 127..134
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 137..144
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 147..152
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 159..162
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 164..166
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 169..180
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 183..187
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 191..194
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 209..221
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 227..240
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 245..252
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 258..266
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 271..281
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 287..290
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 293..299
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 302..308
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 329..333
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 339..341
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 347..358
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 359..361
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 364..366
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 374..383
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 386..390
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 402..404
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 406..408
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 410..412
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 413..427
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 433..437
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 446..458
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 461..465
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 467..470
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 479..485
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 488..495
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 503..509
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 512..514
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 519..526
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 532..538
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 545..548
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 554..557
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 561..563
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 569..572
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 586..594
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 596..603
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 606..611
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 618..620
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 621..629
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 632..639
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 648..652
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 663..673
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 676..678
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 683..692
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 697..699
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 706..709
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 711..718
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 721..726
FT /evidence="ECO:0007829|PDB:6S6Z"
FT TURN 727..729
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 731..737
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 744..750
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 757..761
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 764..767
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 769..774
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 779..787
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 789..799
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 805..813
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 818..825
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 834..843
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 848..857
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 868..875
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 876..878
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 892..901
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 906..922
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 924..929
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 933..935
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 940..950
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 958..960
FT /evidence="ECO:0007829|PDB:6S6Z"
FT HELIX 965..967
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 974..985
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 991..993
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1000..1004
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1007..1009
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1015..1023
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1025..1027
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1029..1037
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1040..1049
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1053..1061
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1066..1073
FT /evidence="ECO:0007829|PDB:6S6Z"
FT STRAND 1078..1083
FT /evidence="ECO:0007829|PDB:6S6Z"
SQ SEQUENCE 1084 AA; 127608 MW; D52E3B762B53DDFC CRC64;
MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF AKNPFEVPED
FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN PPFVPKDDNP TGVYRRWIEI
PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG FSKDSCTPAE FRLTDVLRPG KNLITVEVLK
WSDGSYLEDQ DMWWFAGIYR DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG
EEEEKDLEVT LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG
EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM IQDIKLMKQH
NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW DPEVTLANRW EWEKAHFDRI
KRMVERDKNH PSIIFWSLGN EAGDGVNFEK AALWIKKRDN TRLIHYEGTT RRGESYYVDV
FSLMYPKMDI LLEYASKKRE KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW
VDQGIRKKDE NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR
QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL LKIPLPEMDD
SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS ISDGVSLRED GKHLTVEAKD
TVYVFSKLTG LLEQILHRRK KILKSPVVPN FWRVPTDNDI GNRMPQRLAI WKRASKERKL
FKMHWKKEEN RVSVHSVFQL PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF
TVPEEFGTVE WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL
SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ MGLGGDDSWG
AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH VEMSSEDVIR EGDTLRVKFS
LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP PFKKEELVFK VEGLKKGEHL IHTNLNTRKT
IYVR
