ID   SELTB_DANRE             Reviewed;         193 AA.
AC   Q6PHY8; Q802G5;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Thioredoxin reductase-like selenoprotein T1b {ECO:0000305};
DE            EC=1.8.1.9 {ECO:0000250|UniProtKB:Q1H5H1};
DE   Flags: Precursor;
GN   Name=selenot1b {ECO:0000305};
GN   Synonyms=selt1b {ECO:0000312|EMBL:AAH53147.2};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1] {ECO:0000312|EMBL:AAH53147.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney {ECO:0000312|EMBL:AAH53147.2};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305, ECO:0000312|EMBL:AAO86700.1}
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-193, AND TISSUE SPECIFICITY.
RC   TISSUE=Kidney {ECO:0000269|PubMed:12915322};
RX   PubMed=12915322; DOI=10.1016/s1567-133x(03)00054-1;
RA   Thisse C., Degrave A., Kryukov G.V., Gladyshev V.N., Obrecht-Pflumio S.,
RA   Krol A., Thisse B., Lescure A.;
RT   "Spatial and temporal expression patterns of selenoprotein genes during
RT   embryogenesis in zebrafish.";
RL   Gene Expr. Patterns 3:525-532(2003).
CC   -!- FUNCTION: Selenoprotein with thioredoxin reductase-like oxidoreductase
CC       activity. {ECO:0000250|UniProtKB:Q1H5H1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide +
CC         H(+) + NADPH; Xref=Rhea:RHEA:20345, Rhea:RHEA-COMP:10698, Rhea:RHEA-
CC         COMP:10700, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.9;
CC         Evidence={ECO:0000250|UniProtKB:Q1H5H1};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q1H5H1}.
CC   -!- TISSUE SPECIFICITY: Widely expressed in the embryo. High level in
CC       embryonic blood at 24 hours post-fertilization (hpf).
CC       {ECO:0000269|PubMed:12915322}.
CC   -!- PTM: May contain a selenide-sulfide bond between Cys-44 and Sec-47.
CC       This bond is speculated to serve as redox-active pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SelWTH family. Selenoprotein T subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH53147.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAO86700.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BC053147; AAH53147.2; ALT_INIT; mRNA.
DR   EMBL; AY216586; AAO86700.1; ALT_INIT; mRNA.
DR   RefSeq; NP_840077.3; NM_178292.5.
DR   STRING; 7955.ENSDARP00000120681; -.
DR   PaxDb; Q6PHY8; -.
DR   PRIDE; Q6PHY8; -.
DR   Ensembl; ENSDART00000146972; ENSDARP00000120681; ENSDARG00000027595.
DR   GeneID; 352921; -.
DR   KEGG; dre:352921; -.
DR   CTD; 352921; -.
DR   ZFIN; ZDB-GENE-030411-1; selenot1b.
DR   eggNOG; KOG3286; Eukaryota.
DR   GeneTree; ENSGT00390000011725; -.
DR   HOGENOM; CLU_113870_1_0_1; -.
DR   InParanoid; Q6PHY8; -.
DR   OrthoDB; 1542197at2759; -.
DR   PhylomeDB; Q6PHY8; -.
DR   TreeFam; TF321235; -.
DR   PRO; PR:Q6PHY8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 18.
DR   Bgee; ENSDARG00000027595; Expressed in mature ovarian follicle and 27 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0004791; F:thioredoxin-disulfide reductase activity; IBA:GO_Central.
DR   GO; GO:0045454; P:cell redox homeostasis; IBA:GO_Central.
DR   InterPro; IPR011893; Selenoprotein_Rdx-typ.
DR   InterPro; IPR019389; Selenoprotein_T.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR13544; PTHR13544; 1.
DR   Pfam; PF10262; Rdx; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR02174; CXXU_selWTH; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; NADP; Oxidoreductase; Redox-active center;
KW   Reference proteome; Selenocysteine; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..193
FT                   /note="Thioredoxin reductase-like selenoprotein T1b"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000252042"
FT   NON_STD         47
FT                   /note="Selenocysteine"
FT                   /evidence="ECO:0000312|EMBL:AAH53147.2"
FT   CROSSLNK        44..47
FT                   /note="Cysteinyl-selenocysteine (Cys-Sec)"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        53
FT                   /note="F -> L (in Ref. 2; AAO86700)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="I -> F (in Ref. 2; AAO86700)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   193 AA;  22386 MW;  6BFD3994A4324031 CRC64;
     METRCLYLLL VCVLSVNHAT ADNGSIKKMK MQYTGFPLLK FQICVSUGYR RVFEEYTRVL
     TQRYPDIRIE GENFLPQPLY RHIASFLSVF KLVVIGLIIL GKNPFTYLHI ETPGIWLWAQ
     ENKIYACTMV FFLSNMIENQ CMSTGAFEVT LNDVPVWSKL QSGHLPSMQQ LVQILENEMK
     LSVHMDSLPH RRA