ID   SELX_STAAE              Reviewed;         203 AA.
AC   A0A0H3K6X4;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Enterotoxin-like toxin X {ECO:0000303|PubMed:28880920};
GN   Name=selX {ECO:0000303|PubMed:28880920}; OrderedLocusNames=NWMN_0362;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
RN   [2]
RP   FUNCTION, DOMAIN, AND MUTAGENESIS OF GLU-167; LYS-170 AND GLN-173.
RX   PubMed=28880920; DOI=10.1371/journal.ppat.1006461;
RA   Tuffs S.W., James D.B.A., Bestebroer J., Richards A.C., Goncheva M.I.,
RA   O'Shea M., Wee B.A., Seo K.S., Schlievert P.M., Lengeling A.,
RA   van Strijp J.A., Torres V.J., Fitzgerald J.R.;
RT   "The Staphylococcus aureus superantigen SElX is a bifunctional toxin that
RT   inhibits neutrophil function.";
RL   PLoS Pathog. 13:E1006461-E1006461(2017).
CC   -!- FUNCTION: Plays a role in the inhibition of the host innate immune
CC       system. Inhibits phagocytosis and killing by human neutrophils by
CC       interacting with multiple neutrophil surface glycoproteins in a sialic
CC       acid-dependent manner. {ECO:0000269|PubMed:28880920}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- INDUCTION: Under the control of the Staphylococcus aureus exotoxin
CC       expression (Sae) two component gene regulatory system.
CC       {ECO:0000250|UniProtKB:G0Z026}.
CC   -!- DOMAIN: The C-terminal domain contains a V-shape binding site for
CC       sialyl Lewis X. {ECO:0000269|PubMed:28880920}.
CC   -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC       {ECO:0000305}.
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DR   EMBL; AP009351; BAF66634.1; -; Genomic_DNA.
DR   RefSeq; WP_000475326.1; NZ_CP023390.1.
DR   AlphaFoldDB; A0A0H3K6X4; -.
DR   SMR; A0A0H3K6X4; -.
DR   EnsemblBacteria; BAF66634; BAF66634; NWMN_0362.
DR   KEGG; sae:NWMN_0362; -.
DR   HOGENOM; CLU_127691_0_0_9; -.
DR   OMA; HLEGTYR; -.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR016091; SuperAg_toxin_C.
DR   SUPFAM; SSF54334; SSF54334; 1.
PE   1: Evidence at protein level;
KW   Secreted.
FT   CHAIN           1..203
FT                   /note="Enterotoxin-like toxin X"
FT                   /id="PRO_0000447512"
FT   REGION          164..180
FT                   /note="Sialic acid-binding motif"
FT                   /evidence="ECO:0000305|PubMed:28880920"
FT   MUTAGEN         167
FT                   /note="E->A: Loss of interaction with neutrophils."
FT                   /evidence="ECO:0000269|PubMed:28880920"
FT   MUTAGEN         170
FT                   /note="K->A: Loss of interaction with neutrophils."
FT                   /evidence="ECO:0000269|PubMed:28880920"
FT   MUTAGEN         173
FT                   /note="Q->A: Loss of interaction with neutrophils."
FT                   /evidence="ECO:0000269|PubMed:28880920"
SQ   SEQUENCE   203 AA;  23165 MW;  3E297272D8E9B772 CRC64;
     MFKKYDSKNS IVLKSILSLG IIYGGTFGIY PKADASTQNS SSVQDKQLQK VEEVPNNSEK
     ALVKKLYDRY SKDTINGKSN KSRNWVYSER PLNENQVRIH LEGTYTVAGR VYTPKRNITL
     NKEVVTLKEL DHIIRFAHIS YGLYMGEHLP KGNIVINTKD GGKYTLESHK ELQKDRENVK
     INTADIKNVT FKLVKSVNDI EQV