ID   SELX_STAAU              Reviewed;         203 AA.
AC   G0Z026;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Enterotoxin-like toxin X {ECO:0000303|PubMed:28880913};
GN   Name=selX {ECO:0000303|PubMed:28880913};
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=951;
RX   PubMed=22022262; DOI=10.1371/journal.ppat.1002271;
RA   Wilson G.J., Seo K.S., Cartwright R.A., Connelley T., Chuang-Smith O.N.,
RA   Merriman J.A., Guinane C.M., Park J.Y., Bohach G.A., Schlievert P.M.,
RA   Morrison W.I., Fitzgerald J.R.;
RT   "A novel core genome-encoded superantigen contributes to lethality of
RT   community-associated MRSA necrotizing pneumonia.";
RL   PLoS Pathog. 7:E1002271-E1002271(2011).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 36-203, FUNCTION, MUTAGENESIS OF
RP   THR-165 AND ARG-176, AND INDUCTION BY SAE.
RX   PubMed=28880913; DOI=10.1371/journal.ppat.1006549;
RA   Langley R.J., Ting Y.T., Clow F., Young P.G., Radcliff F.J., Choi J.M.,
RA   Sequeira R.P., Holtfreter S., Baker H., Fraser J.D.;
RT   "Staphylococcal enterotoxin-like X (SElX) is a unique superantigen with
RT   functional features of two major families of staphylococcal virulence
RT   factors.";
RL   PLoS Pathog. 13:E1006549-E1006549(2017).
CC   -!- FUNCTION: Plays a role in the inhibition of the host innate immune
CC       system. Inhibits phagocytosis and killing by human neutrophils by
CC       interacting with multiple neutrophil surface glycoproteins in a sialic
CC       acid-dependent manner. {ECO:0000269|PubMed:28880913}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- INDUCTION: Under the control of the Staphylococcus aureus exotoxin
CC       expression (Sae) two component gene regulatory system.
CC       {ECO:0000269|PubMed:28880913}.
CC   -!- DOMAIN: The C-terminal domain contains a V-shape binding site for
CC       sialyl Lewis X. {ECO:0000269|PubMed:28880913}.
CC   -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC       {ECO:0000305}.
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DR   EMBL; HQ850970; AEI60187.1; -; Genomic_DNA.
DR   RefSeq; WP_000475329.1; NZ_WSFM01000002.1.
DR   PDB; 5U75; X-ray; 1.66 A; A=36-203.
DR   PDBsum; 5U75; -.
DR   AlphaFoldDB; G0Z026; -.
DR   SMR; G0Z026; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR016091; SuperAg_toxin_C.
DR   SUPFAM; SSF54334; SSF54334; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Secreted.
FT   CHAIN           1..203
FT                   /note="Enterotoxin-like toxin X"
FT                   /id="PRO_0000447514"
FT   REGION          164..180
FT                   /note="Sialic acid-binding motif"
FT                   /evidence="ECO:0000305|PubMed:28880913"
FT   MUTAGEN         165
FT                   /note="T->A: Loss of binding to human granulocytes and
FT                   monocytes."
FT                   /evidence="ECO:0000269|PubMed:28880913"
FT   MUTAGEN         176
FT                   /note="R->A: Loss of binding to human granulocytes and
FT                   monocytes."
FT                   /evidence="ECO:0000269|PubMed:28880913"
FT   HELIX           58..70
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          73..75
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   TURN            82..86
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          96..107
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          110..112
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          116..126
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   HELIX           127..139
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   TURN            140..144
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          146..148
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          151..158
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          163..167
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   HELIX           174..176
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          180..182
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   HELIX           183..185
FT                   /evidence="ECO:0007829|PDB:5U75"
FT   STRAND          186..195
FT                   /evidence="ECO:0007829|PDB:5U75"
SQ   SEQUENCE   203 AA;  23225 MW;  0DC5367CDED88E22 CRC64;
     MFKKYDSKNS IVLKSILSLG IIYSGSFGIY PKADASTQNS SSVQDKQLQK VEEVPNNSEK
     ALVKKLYDRY SKDTINGKSN KSRNWVYSER PLNENQVRIH LEGTYTVAGR VYTPKRNITL
     NKEVVTLKEL DHIIRFAHIS YGLYMGEHLP KGNIVINTKN GGKYTLESHK ELQKNRENVE
     INTDDIKNVT FELVKSVNDI EQV