SEM4G_MOUSE
ID SEM4G_MOUSE Reviewed; 837 AA.
AC Q9WUH7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Semaphorin-4G;
DE Flags: Precursor;
GN Name=Sema4g;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=10495281; DOI=10.1016/s0925-4773(99)00125-2;
RA Li H., Wu D.K., Sullivan S.L.;
RT "Characterization and expression of sema4g, a novel member of the
RT semaphorin gene family.";
RL Mech. Dev. 87:169-173(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-836, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-836, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, INTERACTION WITH PLXNB2, AND DISRUPTION PHENOTYPE.
RX PubMed=21122816; DOI=10.1016/j.mcn.2010.11.005;
RA Maier V., Jolicoeur C., Rayburn H., Takegahara N., Kumanogoh A.,
RA Kikutani H., Tessier-Lavigne M., Wurst W., Friedel R.H.;
RT "Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar
RT development.";
RL Mol. Cell. Neurosci. 46:419-431(2011).
CC -!- FUNCTION: Cell surface receptor for PLXNB2. May play a role in axon
CC guidance. {ECO:0000269|PubMed:21122816}.
CC -!- SUBUNIT: Interacts with PLXNB2. {ECO:0000269|PubMed:21122816}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane
CC protein.
CC -!- TISSUE SPECIFICITY: Brain, spinal cord, and several sensory organs as
CC well as specific populations of projection neurons.
CC -!- DEVELOPMENTAL STAGE: Expressed early in development.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:21122816}.
CC -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
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DR EMBL; AF134918; AAD30541.1; -; mRNA.
DR EMBL; BC049183; AAH49183.1; -; mRNA.
DR CCDS; CCDS29853.1; -.
DR RefSeq; NP_036106.1; NM_011976.1.
DR AlphaFoldDB; Q9WUH7; -.
DR SMR; Q9WUH7; -.
DR BioGRID; 205002; 4.
DR MINT; Q9WUH7; -.
DR STRING; 10090.ENSMUSP00000026225; -.
DR GlyConnect; 2700; 1 N-Linked glycan (1 site).
DR GlyGen; Q9WUH7; 6 sites, 1 N-linked glycan (1 site).
DR iPTMnet; Q9WUH7; -.
DR PhosphoSitePlus; Q9WUH7; -.
DR jPOST; Q9WUH7; -.
DR MaxQB; Q9WUH7; -.
DR PaxDb; Q9WUH7; -.
DR PeptideAtlas; Q9WUH7; -.
DR PRIDE; Q9WUH7; -.
DR ProteomicsDB; 256772; -.
DR Antibodypedia; 31221; 63 antibodies from 17 providers.
DR DNASU; 26456; -.
DR Ensembl; ENSMUST00000026225; ENSMUSP00000026225; ENSMUSG00000025207.
DR Ensembl; ENSMUST00000130549; ENSMUSP00000138321; ENSMUSG00000025207.
DR Ensembl; ENSMUST00000179305; ENSMUSP00000137395; ENSMUSG00000025207.
DR GeneID; 26456; -.
DR KEGG; mmu:26456; -.
DR UCSC; uc008hqf.1; mouse.
DR CTD; 57715; -.
DR MGI; MGI:1347047; Sema4g.
DR VEuPathDB; HostDB:ENSMUSG00000025207; -.
DR eggNOG; KOG3611; Eukaryota.
DR GeneTree; ENSGT00940000157186; -.
DR HOGENOM; CLU_009051_4_2_1; -.
DR InParanoid; Q9WUH7; -.
DR OMA; SGPYMEY; -.
DR OrthoDB; 176445at2759; -.
DR PhylomeDB; Q9WUH7; -.
DR TreeFam; TF316102; -.
DR BioGRID-ORCS; 26456; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Sema4g; mouse.
DR PRO; PR:Q9WUH7; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9WUH7; protein.
DR Bgee; ENSMUSG00000025207; Expressed in left lobe of liver and 197 other tissues.
DR Genevisible; Q9WUH7; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0045499; F:chemorepellent activity; IBA:GO_Central.
DR GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR GO; GO:0007411; P:axon guidance; IBA:GO_Central.
DR GO; GO:0050919; P:negative chemotaxis; IBA:GO_Central.
DR GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IBA:GO_Central.
DR GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR GO; GO:0071526; P:semaphorin-plexin signaling pathway; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR002165; Plexin_repeat.
DR InterPro; IPR016201; PSI.
DR InterPro; IPR001627; Semap_dom.
DR InterPro; IPR036352; Semap_dom_sf.
DR InterPro; IPR027231; Semaphorin.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR11036; PTHR11036; 1.
DR Pfam; PF01437; PSI; 1.
DR Pfam; PF01403; Sema; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00423; PSI; 1.
DR SMART; SM00630; Sema; 1.
DR SUPFAM; SSF101912; SSF101912; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS51004; SEMA; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Developmental protein; Differentiation; Disulfide bond;
KW Glycoprotein; Immunoglobulin domain; Membrane; Neurogenesis;
KW Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..837
FT /note="Semaphorin-4G"
FT /id="PRO_0000032334"
FT TOPO_DOM 18..673
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 674..694
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 695..837
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 35..503
FT /note="Sema"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00352"
FT DOMAIN 505..556
FT /note="PSI"
FT DOMAIN 565..647
FT /note="Ig-like C2-type"
FT REGION 721..776
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 759..776
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 794
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTN9"
FT MOD_RES 836
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 386
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 540
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 596
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 104..115
FT /evidence="ECO:0000250"
FT DISULFID 133..142
FT /evidence="ECO:0000250"
FT DISULFID 268..375
FT /evidence="ECO:0000250"
FT DISULFID 292..335
FT /evidence="ECO:0000250"
FT DISULFID 506..523
FT /evidence="ECO:0000250"
FT DISULFID 515..532
FT /evidence="ECO:0000250"
FT DISULFID 582..630
FT /evidence="ECO:0000250"
SQ SEQUENCE 837 AA; 92378 MW; 5C6E9C9C8A545EB1 CRC64;
MWGRLWPLLF SFLTVTAVPG PSLRRPSREL DATPRLTISY EELSQIRHFK GQTQNYSTLL
LEEASERLLV GARGALFSLS ARDIRDRTHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR
FLQRLNATHF YACGTHAFQP LCAAIDAETF ILPTSFEEGK EKCPYDPARG FTGLIIDGGL
YTATRYEFRS IPDIRRSRHP HSLRTEEAPM HWLNDAEFVF SVLVRESKTS AVGDDDKIYF
FFMEREEGSS SFTQSRSSHR VARVARVCKG DLGGKKILQK KWTSFLKARL ICHIPQYETL
RGVCSLNADT SSHTHFYAVF TLTTQWKTLE ASAICRYDLA EIQAVFTGPF MEYQDGARRW
GRYEGGVPEP RPGSCITDSL RSRGYNSSQD LPSLVLDFVK LHPLMARPVV PTRGRPLLLK
RNVRYTHLTG THVSTPAGPT YDLLFLGTAD GWIHKAVVLG SGMHIIEEIQ VFREPQSVDN
LVISPMQHSL YVGAASGVLQ FPLSSCSRYQ SCYDCILARD PYCGWDSSIH ACMVATTVAN
RTELIQDIER GNRGCEGSRD AGPPPPLKTR SVLRGDDVLL PCDQPSNLAR ALWLLNGSKS
LSDGQDGYRV GVDGLLVTDT QLEHSGNYGC YAEENGLRML LASYSLTVRP ATPAPAPQAP
ATPGAQLAHD MRMFYVVAIA ILGGLCLILA SSLLYVACLK GGRRGRRRKY SLGRAGRAGG
SAVQLQTVSG QCPGEEDEGD DGEGTGGLES GCLQIIPGEG APAPPPPPPP PPPAELTNGL
VALPSRLRRM NGNSYVLLRQ SNNGVPAGPC SFAEELSRIL EKRKHTQLVE QLDESSV
