SEM5B_MOUSE
ID SEM5B_MOUSE Reviewed; 1093 AA.
AC Q60519; B1B1A9; Q6ZPQ8; Q7TT33; Q8BVE5;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Semaphorin-5B;
DE AltName: Full=Semaphorin-G;
DE Short=Sema G;
DE Flags: Precursor;
GN Name=Sema5b; Synonyms=Kiaa1445, Semag, SemG;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=NMRI;
RX PubMed=8817451; DOI=10.1016/0925-4773(96)00525-4;
RA Adams R.H., Betz H., Pueschel A.W.;
RT "A novel class of murine semaphorins with homology to thrombospondin is
RT differentially expressed during early embryogenesis.";
RL Mech. Dev. 57:33-45(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 462-1093 (ISOFORM 1).
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 594-1093 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Adrenal gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: May act as positive axonal guidance cues.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q60519-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q60519-2; Sequence=VSP_029467, VSP_029468;
CC Name=3;
CC IsoId=Q60519-3; Sequence=VSP_029466;
CC -!- TISSUE SPECIFICITY: In adult, only detected in brain.
CC -!- DEVELOPMENTAL STAGE: Differentially expressed in embryonic and adult
CC tissues. Its abundance decreases from 10 dpc to birth.
CC -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
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DR EMBL; X97818; CAA66398.1; -; mRNA.
DR EMBL; AC154653; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT010576; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466521; EDK97889.1; -; Genomic_DNA.
DR EMBL; BC052397; AAH52397.1; -; mRNA.
DR EMBL; AK129362; BAC98172.1; -; mRNA.
DR EMBL; AK078659; BAC37350.1; -; mRNA.
DR CCDS; CCDS28140.1; -. [Q60519-1]
DR RefSeq; NP_038689.2; NM_013661.2. [Q60519-1]
DR RefSeq; XP_006521932.1; XM_006521869.1. [Q60519-3]
DR RefSeq; XP_011244146.1; XM_011245844.1. [Q60519-3]
DR AlphaFoldDB; Q60519; -.
DR SMR; Q60519; -.
DR BioGRID; 203172; 4.
DR STRING; 10090.ENSMUSP00000057494; -.
DR GlyGen; Q60519; 13 sites.
DR iPTMnet; Q60519; -.
DR PhosphoSitePlus; Q60519; -.
DR MaxQB; Q60519; -.
DR PaxDb; Q60519; -.
DR PRIDE; Q60519; -.
DR ProteomicsDB; 256945; -. [Q60519-1]
DR ProteomicsDB; 256946; -. [Q60519-2]
DR ProteomicsDB; 256947; -. [Q60519-3]
DR Antibodypedia; 2250; 121 antibodies from 14 providers.
DR DNASU; 20357; -.
DR Ensembl; ENSMUST00000050625; ENSMUSP00000057494; ENSMUSG00000052133. [Q60519-1]
DR Ensembl; ENSMUST00000120756; ENSMUSP00000112536; ENSMUSG00000052133. [Q60519-3]
DR GeneID; 20357; -.
DR KEGG; mmu:20357; -.
DR UCSC; uc007zbm.2; mouse. [Q60519-1]
DR UCSC; uc012aez.1; mouse. [Q60519-3]
DR CTD; 54437; -.
DR MGI; MGI:107555; Sema5b.
DR VEuPathDB; HostDB:ENSMUSG00000052133; -.
DR eggNOG; KOG3611; Eukaryota.
DR GeneTree; ENSGT00940000156712; -.
DR HOGENOM; CLU_005410_1_0_1; -.
DR InParanoid; Q60519; -.
DR OMA; AICAFNM; -.
DR OrthoDB; 64683at2759; -.
DR TreeFam; TF329951; -.
DR Reactome; R-MMU-5173214; O-glycosylation of TSR domain-containing proteins.
DR BioGRID-ORCS; 20357; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Sema5b; mouse.
DR PRO; PR:Q60519; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q60519; protein.
DR Bgee; ENSMUSG00000052133; Expressed in floor plate of midbrain and 135 other tissues.
DR Genevisible; Q60519; MM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0045499; F:chemorepellent activity; IBA:GO_Central.
DR GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR GO; GO:0048675; P:axon extension; IGI:MGI.
DR GO; GO:0007411; P:axon guidance; IBA:GO_Central.
DR GO; GO:0050908; P:detection of light stimulus involved in visual perception; IGI:MGI.
DR GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IBA:GO_Central.
DR GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR GO; GO:1990138; P:neuron projection extension; IDA:MGI.
DR GO; GO:0097485; P:neuron projection guidance; IMP:MGI.
DR GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR GO; GO:0071526; P:semaphorin-plexin signaling pathway; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.20.100.10; -; 5.
DR InterPro; IPR016201; PSI.
DR InterPro; IPR001627; Semap_dom.
DR InterPro; IPR036352; Semap_dom_sf.
DR InterPro; IPR027231; Semaphorin.
DR InterPro; IPR000884; TSP1_rpt.
DR InterPro; IPR036383; TSP1_rpt_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR11036; PTHR11036; 3.
DR Pfam; PF01403; Sema; 1.
DR Pfam; PF00090; TSP_1; 5.
DR SMART; SM00423; PSI; 1.
DR SMART; SM00630; Sema; 1.
DR SMART; SM00209; TSP1; 5.
DR SUPFAM; SSF101912; SSF101912; 1.
DR SUPFAM; SSF82895; SSF82895; 4.
DR PROSITE; PS51004; SEMA; 1.
DR PROSITE; PS50092; TSP1; 5.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation;
KW Disulfide bond; Glycoprotein; Membrane; Neurogenesis; Reference proteome;
KW Repeat; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..1093
FT /note="Semaphorin-5B"
FT /id="PRO_0000032338"
FT TOPO_DOM 20..978
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 979..999
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1000..1093
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 45..495
FT /note="Sema"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00352"
FT DOMAIN 551..605
FT /note="TSP type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 606..662
FT /note="TSP type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 664..713
FT /note="TSP type-1 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 721..776
FT /note="TSP type-1 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 795..850
FT /note="TSP type-1 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 852..907
FT /note="TSP type-1 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 908..952
FT /note="TSP type-1 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 287
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 333
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 378
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 532
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 539
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 547
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 602
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 728
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 944
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 114..124
FT /evidence="ECO:0000250"
FT DISULFID 141..150
FT /evidence="ECO:0000250"
FT DISULFID 264..367
FT /evidence="ECO:0000250"
FT DISULFID 288..330
FT /evidence="ECO:0000250"
FT DISULFID 498..515
FT /evidence="ECO:0000250"
FT DISULFID 507..524
FT /evidence="ECO:0000250"
FT DISULFID 618..655
FT /evidence="ECO:0000250"
FT DISULFID 622..661
FT /evidence="ECO:0000250"
FT DISULFID 633..645
FT /evidence="ECO:0000250"
FT DISULFID 676..707
FT /evidence="ECO:0000250"
FT DISULFID 680..712
FT /evidence="ECO:0000250"
FT DISULFID 691..697
FT /evidence="ECO:0000250"
FT DISULFID 807..844
FT /evidence="ECO:0000250"
FT DISULFID 811..849
FT /evidence="ECO:0000250"
FT DISULFID 822..834
FT /evidence="ECO:0000250"
FT DISULFID 864..901
FT /evidence="ECO:0000250"
FT DISULFID 868..906
FT /evidence="ECO:0000250"
FT DISULFID 879..891
FT /evidence="ECO:0000250"
FT VAR_SEQ 702
FT /note="V -> VVRSRGWGAGSGETYSPGGVGGSEATLTPK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029466"
FT VAR_SEQ 908
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029467"
FT VAR_SEQ 1041..1093
FT /note="KTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPN
FT S -> TSEATRPSKGNIRCGPTLSSSCVELGNPAGGH (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029468"
FT CONFLICT 84
FT /note="A -> G (in Ref. 1; CAA66398)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="V -> I (in Ref. 1; CAA66398)"
FT /evidence="ECO:0000305"
FT CONFLICT 791
FT /note="P -> Q (in Ref. 6; BAC37350)"
FT /evidence="ECO:0000305"
FT CONFLICT 1006
FT /note="R -> S (in Ref. 6; BAC37350)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1093 AA; 120327 MW; 512D73EEBC8959D2 CRC64;
MVVPGPLALS LLLSSLTLLV SHLSSSQDIA SESSSEQQMC TRREHPIVAF EDLKPWVFNF
TYPGVRDFSQ LALDPSRNQL IVGARNYLFR LSLANVSLLQ ATEWASSEDT RRSCQSKGKT
EEECQNYVRV LIVSGRKVFM CGTNAFSPVC SSRQVGNLSR TIEKINGVAR CPYDPRHNST
AVISSQGELY AATVIDFSGR DPAIYRSLGS GPPLRTAQYN SKWLNEPNFV AAFDIGLFAY
FFLRENAVEH DCGRTVYSRV ARVCKNDVGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE
LQSAFHLPEQ DLIYGVFTTN VNSIAASAVC AFNLSAISKA FNGPFRYQEN PRAAWLPIAN
PIPNFQCGTL PETGPNENLT ERSLQDAQRL FLMSEAVQPV TPEPCVTQDS VRFSHLVVDL
VQAKDTLYHV LYIGTESGTI LKALSTASRS LRGCYLEELH VLPPGRLEPL RSLRILHSAR
ALFVGLSDRV LRVPLERCSA YHSQGACLGA RDPYCGWDGK RQLCSTLEDS SNMSLWIQNI
TTCPVRNVTR DGGFGPWSPW KPCEHLDGDN SGSCLCRARS CDSPRPRCGG LECLGPSIHI
ANCSRNGAWT AWSSWAQCST SCGIGFQVRQ RSCSNPAPRH GGRICVGKSR EERFCNENTP
CPVPIFWASW GSWSKCSNNC GGGVQSRRRS CENGNSCPGC GVEFKTCNPE ACPEVRRNTP
WTPWLPVNVT QGGARQEQRF RFTCRAPLPD PHGLQFGKRR TETRTCPADG TGACDTDALV
EDLLRSGSTS PHTLNGGWAT WGPWSSCSRD CELGFRVRKR TCTNPEPRNG GLPCVGDAAE
YQDCNPQACP VRGAWSCWTA WSQCSASCGG GHYQRTRSCT SPAPSPGEDI CLGLHTEEAL
CSTQACPEGW SLWSEWGVCT EDGAQSRSRS CEELLPGPGA CVGNSSQSRP CPYSEIPVIL
PASSVEETTS CGGFNLIHLI VTGVSCFLVS GLLTLAVYLS CQHCQRQSQE STLVHPATPN
HLHYKGGGTP KNEKYTPMEF KTLNKNNLIP DDRANFYPLQ QTNVYTTTYY PSPLNKPSFR
PEASPGQRCF PNS
