BGCN_DROME
ID BGCN_DROME Reviewed; 1215 AA.
AC Q9W1I2; Q9W1I3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Benign gonial cell neoplasm protein;
GN Name=bgcn; Synonyms=b(2)gcn; ORFNames=CG30170;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=10924476; DOI=10.1093/genetics/155.4.1809;
RA Ohlstein B., Lavoie C.A., Vef O., Gateff E., McKearin D.M.;
RT "The Drosophila cystoblast differentiation factor, benign gonial cell
RT neoplasm, is related to DExH-box proteins and interacts genetically with
RT bag-of-marbles.";
RL Genetics 155:1809-1819(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-898, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Essential cystoblast differentiation factor required for Bam
CC function in asymmetric division of the germline stem cells.
CC -!- INTERACTION:
CC Q9W1I2; P22745: bam; NbExp=13; IntAct=EBI-142483, EBI-88504;
CC -!- TISSUE SPECIFICITY: Expressed in testis and in 5-8 germline stem cells
CC of ovaries, immediately adjacent to terminal filament.
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DR EMBL; AF255662; AAF91348.1; -; mRNA.
DR EMBL; AE013599; AAF47077.2; -; Genomic_DNA.
DR RefSeq; NP_523832.2; NM_079108.3.
DR AlphaFoldDB; Q9W1I2; -.
DR SMR; Q9W1I2; -.
DR BioGRID; 71021; 13.
DR DIP; DIP-48890N; -.
DR IntAct; Q9W1I2; 1.
DR STRING; 7227.FBpp0072141; -.
DR iPTMnet; Q9W1I2; -.
DR PaxDb; Q9W1I2; -.
DR PRIDE; Q9W1I2; -.
DR EnsemblMetazoa; FBtr0072232; FBpp0072141; FBgn0004581.
DR GeneID; 47873; -.
DR KEGG; dme:Dmel_CG30170; -.
DR CTD; 47873; -.
DR FlyBase; FBgn0004581; bgcn.
DR VEuPathDB; VectorBase:FBgn0004581; -.
DR eggNOG; KOG0920; Eukaryota.
DR InParanoid; Q9W1I2; -.
DR OMA; DIDHLWD; -.
DR OrthoDB; 264013at2759; -.
DR PhylomeDB; Q9W1I2; -.
DR BioGRID-ORCS; 47873; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 47873; -.
DR PRO; PR:Q9W1I2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0004581; Expressed in testis and 8 other tissues.
DR ExpressionAtlas; Q9W1I2; baseline and differential.
DR Genevisible; Q9W1I2; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0002151; F:G-quadruplex RNA binding; IBA:GO_Central.
DR GO; GO:0004386; F:helicase activity; ISS:FlyBase.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; IDA:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0035212; P:cell competition in a multicellular organism; IMP:FlyBase.
DR GO; GO:0007282; P:cystoblast division; TAS:FlyBase.
DR GO; GO:0007281; P:germ cell development; TAS:FlyBase.
DR GO; GO:0030718; P:germ-line stem cell population maintenance; IDA:FlyBase.
DR GO; GO:0098730; P:male germline stem cell symmetric division; IMP:FlyBase.
DR GO; GO:0007140; P:male meiotic nuclear division; TAS:FlyBase.
DR GO; GO:0017148; P:negative regulation of translation; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; HMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; TAS:FlyBase.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00847; HA2; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Developmental protein; Phosphoprotein; Reference proteome.
FT CHAIN 1..1215
FT /note="Benign gonial cell neoplasm protein"
FT /id="PRO_0000067054"
FT REPEAT 407..439
FT /note="ANK"
FT MOD_RES 898
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1215 AA; 139279 MW; 11F83C1B3816F2CD CRC64;
MNHIIQDKYI PQQLLYFLAG RRCCQQFPCT FRTSEHEAFA NNARSLGLRS QVVHVNGNSC
VKVYKQACRH YLEEPKTLVL SSGATLNMFT LLSRKSLMGK EDLELYADLV SMKANASDLP
SLHLPLPAIR PPNLRFWTEA QLNFLTAFLG HSLSDEILQS LYASRVIVYN AALCWDKSVF
LPLVILDDCR NKKSNVKIMC IERQAILATY NSQRTANFFG EQLGETVGIQ LPYFSAVSSS
TFIIYSTAQY FLRSLTSQQF RNISHLVVND VHLHDPYTDI LLSEIRMALS SHQNLRVVLL
SQMGNPKKFT DFFGEGLQLN MIKQPEVAPR VSYLNELHSC IALAGIHKGP DIYKEIPEAF
RANNPRNEQM DKCLQAYGEL GTDAALRPFL YAVNYDLAPV NYRHSLTGKT AVHFASELNK
ANHLRLLLFM GADPYIVDLF QQNAISLAAM NGNHECIDVL NSYSLHGYVV KSAKPDFVDY
DLIIDIMYLL RTKPEYSPGE YSPGNILIIL PTYYHIVKLN YMILSHCLTG SLQECSIFLL
YDNMRNDYLQ ALVNASDETV KVVLATDIIE SLCLKVPFKY QIDTACRLNN VYDTTSCSGD
DRFEWVAKDA LLRRELILQP NKGDVQCFRL ISKEAYEELS ETSQPSLQTM QLDKICLAVK
LLSPNTIISE YLGITISPPP LINVHHAVQF LKKIDVLDDA EDVTWLGCRL MDIPVSCQLG
RMLIFGILLR CLDPILTIVS SLSTADPLGI PFTEDIDNLW DRFTIYIQNS IKKERTYLSD
NQFSDHFIFV RLYKEWQNRM HNRTPPLYLK DEYEFMLNGL MEQLTSIRSE IVSSLRAANL
IHSRGKLSMN NLNQMSCNWH MVKAALTGGM YPNIYAVDTR KSSLKSAFSG NVSMHPNTVL
RDFLEPLNIS AQSFRTPWIV CNRQKSHIVY ATLVVPLAVA MFSGHPRIRL SPICDSEMSL
TDRNVNVFID EWIWMVMSKA TAEMVMRTRY YFFKMYHDLL KHCSELDMWR RDCEPVSQYT
VLTDTLSKIF ESEDGFVGFF KPPPITFLPT PQLPSLYLLS VNAHFSWARE VEENMLSKPH
HFNSHFIERQ FFVLYAGGDC EEFHSRNTPA FIESVLGKFV RPIDTPNRHI FVILYRKDPD
MMLSISRAKF VNGVFMLQEY FRNNIPVFEI LDACVSLNVQ TPVFDGRLMS ALIDKRVGNL
IMELFAFRHH WIHKR
