ID   SEN15_NEUCR             Reviewed;         223 AA.
AC   Q7RY78;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Probable tRNA-splicing endonuclease subunit tsp-1;
DE   AltName: Full=tRNA-intron endonuclease sen15;
DE   AltName: Full=tRNA-splicing protein 1;
GN   Name=tsp-1; Synonyms=sen15; ORFNames=NCU00027;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Non-catalytic subunit of the tRNA-splicing endonuclease
CC       complex, a complex responsible for identification and cleavage of the
CC       splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice
CC       sites to release the intron. The products are an intron and two tRNA
CC       half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There
CC       are no conserved sequences at the splice sites, but the intron is
CC       invariably located at the same site in the gene, placing the splice
CC       sites an invariant distance from the constant structural features of
CC       the tRNA body (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC       tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts
CC       directly with tsp-4/sen34 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SEN15 family. {ECO:0000305}.
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DR   EMBL; CM002238; EAA27778.1; -; Genomic_DNA.
DR   RefSeq; XP_957014.1; XM_951921.2.
DR   AlphaFoldDB; Q7RY78; -.
DR   STRING; 5141.EFNCRP00000000006; -.
DR   EnsemblFungi; EAA27778; EAA27778; NCU00027.
DR   GeneID; 3873152; -.
DR   KEGG; ncr:NCU00027; -.
DR   VEuPathDB; FungiDB:NCU00027; -.
DR   HOGENOM; CLU_083361_1_0_1; -.
DR   InParanoid; Q7RY78; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   InterPro; IPR042777; Sen15_fungi.
DR   InterPro; IPR018593; tRNA-endonuc_su_Sen15.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   PANTHER; PTHR28518; PTHR28518; 1.
DR   Pfam; PF09631; Sen15; 1.
DR   SUPFAM; SSF53032; SSF53032; 1.
PE   3: Inferred from homology;
KW   Reference proteome; tRNA processing.
FT   CHAIN           1..223
FT                   /note="Probable tRNA-splicing endonuclease subunit tsp-1"
FT                   /id="PRO_0000194026"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        27..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   223 AA;  25366 MW;  B005C959C41EF4E6 CRC64;
     MESGSTPPTD KQIRENEQDG TGHQGKLLAR PTSTRQQQQQ QQQQPSHSVS QSVSQSPQTQ
     DCLTIVANMS ADQHLQALTK VVVNNLENQH DWTQVQIHIQ DNLPRPLIYG LPPKRLYVHP
     DEQIDIIRAE KQLNQRVPQE PELEWVLPLH LAEKWSISDF AAVFDAITAL PPGGESVNAN
     EDAQWQLWRG PKRGKRILLA TVQDDSTVTY YWMHNGLVKP RQN