SEN21_ARATH
ID SEN21_ARATH Reviewed; 237 AA.
AC Q9M1E8; Q9S7L3;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=tRNA-splicing endonuclease subunit Sen2-1;
DE EC=4.6.1.16;
DE AltName: Full=tRNA-intron endonuclease Sen2-1;
DE Short=AtSen1;
GN Name=SEN1; OrderedLocusNames=At3g45590; ORFNames=F9K21.170;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND VARIANTS LEU-24; GLN-27;
RP GLN-52; SER-67; SER-167; THR-191; 199-GLY--ASN-205 AND 213-ASP-GLU-214.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=11080584; DOI=10.1016/s0378-1119(00)00408-x;
RA Akama K., Junker V., Beier H.;
RT "Identification of two catalytic subunits of tRNA splicing endonuclease
RT from Arabidopsis thaliana.";
RL Gene 257:177-185(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC splicing endonuclease complex, a complex responsible for identification
CC and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC 5'-OH termini. There are no conserved sequences at the splice sites,
CC but the intron is invariably located at the same site in the gene,
CC placing the splice sites an invariant distance from the constant
CC structural features of the tRNA body. Probably carries the active site
CC for 5'-splice site cleavage (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC SEN2, SEN15, SEN34/LENG5 and SEN54. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC {ECO:0000305}.
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DR EMBL; AB036339; BAA88627.1; -; mRNA.
DR EMBL; AB036340; BAA88628.1; -; Genomic_DNA.
DR EMBL; AL138657; CAB75488.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78047.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78048.1; -; Genomic_DNA.
DR EMBL; AK221492; BAD94688.1; -; mRNA.
DR PIR; T47499; T47499.
DR RefSeq; NP_001078245.1; NM_001084776.2.
DR RefSeq; NP_190145.1; NM_114428.4.
DR AlphaFoldDB; Q9M1E8; -.
DR SMR; Q9M1E8; -.
DR STRING; 3702.AT3G45590.1; -.
DR PaxDb; Q9M1E8; -.
DR PRIDE; Q9M1E8; -.
DR ProteomicsDB; 234507; -.
DR EnsemblPlants; AT3G45590.1; AT3G45590.1; AT3G45590.
DR EnsemblPlants; AT3G45590.2; AT3G45590.2; AT3G45590.
DR GeneID; 823701; -.
DR Gramene; AT3G45590.1; AT3G45590.1; AT3G45590.
DR Gramene; AT3G45590.2; AT3G45590.2; AT3G45590.
DR KEGG; ath:AT3G45590; -.
DR Araport; AT3G45590; -.
DR TAIR; locus:2085687; AT3G45590.
DR eggNOG; KOG4685; Eukaryota.
DR HOGENOM; CLU_053228_0_0_1; -.
DR InParanoid; Q9M1E8; -.
DR OMA; KNWIVRS; -.
DR OrthoDB; 1219366at2759; -.
DR PhylomeDB; Q9M1E8; -.
DR BRENDA; 4.6.1.16; 399.
DR PRO; PR:Q9M1E8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1E8; baseline and differential.
DR Genevisible; Q9M1E8; AT.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006678; tRNA_intron_Endonuc_N.
DR InterPro; IPR006676; tRNA_splic.
DR PANTHER; PTHR21227; PTHR21227; 1.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR Pfam; PF02778; tRNA_int_endo_N; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
PE 2: Evidence at transcript level;
KW Lyase; mRNA processing; Nucleus; Reference proteome; tRNA processing.
FT CHAIN 1..237
FT /note="tRNA-splicing endonuclease subunit Sen2-1"
FT /id="PRO_0000109456"
FT ACT_SITE 148
FT /evidence="ECO:0000250"
FT ACT_SITE 156
FT /evidence="ECO:0000250"
FT ACT_SITE 190
FT /evidence="ECO:0000250"
FT VARIANT 24
FT /note="S -> L (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 27
FT /note="R -> Q (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 52
FT /note="E -> Q (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 67
FT /note="G -> S (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 167
FT /note="G -> S (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 191
FT /note="S -> T (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:11080584"
FT VARIANT 199..205
FT /note="RKVNTEK -> GQVKTEN (in strain: cv. Landsberg
FT erecta)"
FT VARIANT 213..214
FT /note="ED -> DE (in strain: cv. Landsberg erecta)"
SQ SEQUENCE 237 AA; 27359 MW; 2844856CB58CD0B2 CRC64;
MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES EEAELLDRCC
FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS LENEVDLWRS MSSFKQDFAI
LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH HPSLVHSEYA VLVQSIGGND RLKVWSDIHC
SVRLTGSVAK SLLVLYVNRK VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT
