SEN22_ARATH
ID SEN22_ARATH Reviewed; 250 AA.
AC Q9LSS3; Q3E887;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=tRNA-splicing endonuclease subunit Sen2-2;
DE EC=4.6.1.16;
DE AltName: Full=tRNA-intron endonuclease Sen2-2;
DE Short=AtSen2;
GN Name=SEN2; OrderedLocusNames=At5g60230; ORFNames=F15L12.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=11080584; DOI=10.1016/s0378-1119(00)00408-x;
RA Akama K., Junker V., Beier H.;
RT "Identification of two catalytic subunits of tRNA splicing endonuclease
RT from Arabidopsis thaliana.";
RL Gene 257:177-185(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC splicing endonuclease complex, a complex responsible for identification
CC and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC 5'-OH termini. There are no conserved sequences at the splice sites,
CC but the intron is invariably located at the same site in the gene,
CC placing the splice sites an invariant distance from the constant
CC structural features of the tRNA body. Probably carries the active site
CC for 5'-splice site cleavage (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC SEN2, SEN15, SEN34/LENG5 and SEN54. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LSS3-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC {ECO:0000305}.
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DR EMBL; AB026632; BAA97504.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97296.1; -; Genomic_DNA.
DR EMBL; BT004112; AAO42135.1; -; mRNA.
DR EMBL; BT004920; AAO50453.1; -; mRNA.
DR RefSeq; NP_200831.1; NM_125416.4. [Q9LSS3-1]
DR AlphaFoldDB; Q9LSS3; -.
DR SMR; Q9LSS3; -.
DR STRING; 3702.AT5G60230.2; -.
DR PaxDb; Q9LSS3; -.
DR PRIDE; Q9LSS3; -.
DR ProteomicsDB; 232876; -. [Q9LSS3-1]
DR EnsemblPlants; AT5G60230.1; AT5G60230.1; AT5G60230. [Q9LSS3-1]
DR GeneID; 836145; -.
DR Gramene; AT5G60230.1; AT5G60230.1; AT5G60230. [Q9LSS3-1]
DR KEGG; ath:AT5G60230; -.
DR Araport; AT5G60230; -.
DR eggNOG; KOG4685; Eukaryota.
DR HOGENOM; CLU_053228_0_0_1; -.
DR InParanoid; Q9LSS3; -.
DR PhylomeDB; Q9LSS3; -.
DR PRO; PR:Q9LSS3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LSS3; baseline and differential.
DR Genevisible; Q9LSS3; AT.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006678; tRNA_intron_Endonuc_N.
DR InterPro; IPR006676; tRNA_splic.
DR PANTHER; PTHR21227; PTHR21227; 1.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR Pfam; PF02778; tRNA_int_endo_N; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Lyase; mRNA processing; Nucleus; Reference proteome;
KW tRNA processing.
FT CHAIN 1..250
FT /note="tRNA-splicing endonuclease subunit Sen2-2"
FT /id="PRO_0000109457"
FT REGION 225..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 148
FT /evidence="ECO:0000250"
FT ACT_SITE 156
FT /evidence="ECO:0000250"
FT ACT_SITE 189
FT /evidence="ECO:0000250"
SQ SEQUENCE 250 AA; 28519 MW; DFAEC30162214014 CRC64;
MAPRWKWKGA EAKALAEPIS KSVSELQLSL AETESSGTLS SCNVLLAVEP EQAELLDRCC
FGRLVLSAEK IKKWIQLSFE EAFFLHYNLK CIKISLQGRC LENEVDTWLY MKSKRPNFPM
FFKAYSHLRS KNWVLRSGLQ YGVDFVAYRH HPSLVHSEYS VLVQSGDSDR LRVWSDIHCA
VRLSGSVAKT LLTLYVNGNF KGEDVNLLVC LENFTVEEQT ISRWSPELSR EDQSTNSKQH
VPNVSNLNTL
