SEN2_ASHGO
ID SEN2_ASHGO Reviewed; 356 AA.
AC Q74ZY5;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=tRNA-splicing endonuclease subunit SEN2;
DE EC=4.6.1.16;
DE AltName: Full=tRNA-intron endonuclease SEN2;
GN Name=SEN2; OrderedLocusNames=AGR073C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 299; 303; 306; 315; 319-320;
RP 324 AND 339-340.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC splicing endonuclease complex, a complex responsible for identification
CC and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC 5'-OH termini. There are no conserved sequences at the splice sites,
CC but the intron is invariably located at the same site in the gene,
CC placing the splice sites an invariant distance from the constant
CC structural features of the tRNA body. This subunit may anchor the
CC endonuclease complex to the nuclear membrane. Probably carries the
CC active site for 5'-splice site cleavage (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC -!- SUBUNIT: Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54.
CC Interacts directly with SEN54 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC {ECO:0000305}.
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DR EMBL; AE016820; AAS54562.2; -; Genomic_DNA.
DR RefSeq; NP_986738.2; NM_211800.2.
DR AlphaFoldDB; Q74ZY5; -.
DR SMR; Q74ZY5; -.
DR STRING; 33169.AAS54562; -.
DR EnsemblFungi; AAS54562; AAS54562; AGOS_AGR073C.
DR GeneID; 4623039; -.
DR KEGG; ago:AGOS_AGR073C; -.
DR eggNOG; KOG4685; Eukaryota.
DR HOGENOM; CLU_012847_2_0_1; -.
DR InParanoid; Q74ZY5; -.
DR OMA; GKYMTSE; -.
DR Proteomes; UP000000591; Chromosome VII.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:EnsemblFungi.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR GO; GO:0098787; P:mRNA cleavage involved in mRNA processing; IEA:EnsemblFungi.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006676; tRNA_splic.
DR InterPro; IPR016589; tRNA_splic_SEN2.
DR PANTHER; PTHR21227; PTHR21227; 1.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR PIRSF; PIRSF011789; tRNA_splic_SEN2; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
DR TIGRFAMs; TIGR00324; endA; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome; tRNA processing.
FT CHAIN 1..356
FT /note="tRNA-splicing endonuclease subunit SEN2"
FT /id="PRO_0000109459"
FT ACT_SITE 268
FT /evidence="ECO:0000250"
FT ACT_SITE 276
FT /evidence="ECO:0000250"
FT ACT_SITE 307
FT /evidence="ECO:0000250"
SQ SEQUENCE 356 AA; 41096 MW; 37FD39B17F5D8D5C CRC64;
MAKRVAGSKR YVHPLPIEPV VLPPLIAHNP LSWVYWVLAY VTSSNQLPRK IPLEVGADGR
YTVTGREQMQ YLWEHGFFGT GQLSRSEPTW QARTVDRLQL DTEGIAGHKL EQVTQLRRKQ
RLEFKRERAS FERKRLELRR QGVLESEILE QERLWLKQLR DRELQWEAST GDPSPVRAED
AEIIAEDGAS VLPIEKLELM PVEALFLTLA LPVLHADAPA ILARTLGPQP ALPQIERLCR
LYAAYHHYRS HGWCVRSGIK FGCDFLLYRR GPPFHHAEFS VMVLAPDERH DYTWYSTVAR
VVGGAQKTLV LAYVARRAAA DQLAALWHAR RYMEAFALFE VHELVYRRWL PGKNRE
