SEN2_CHICK
ID SEN2_CHICK Reviewed; 461 AA.
AC Q5ZIN2;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=tRNA-splicing endonuclease subunit Sen2;
DE EC=4.6.1.16;
DE AltName: Full=tRNA-intron endonuclease Sen2;
GN Name=TSEN2; ORFNames=RCJMB04_24m3;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC splicing endonuclease complex, a complex responsible for identification
CC and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC 5'-OH termini. There are no conserved sequences at the splice sites,
CC but the intron is invariably located at the same site in the gene,
CC placing the splice sites an invariant distance from the constant
CC structural features of the tRNA body. Probably carries the active site
CC for 5'-splice site cleavage. The tRNA splicing endonuclease is also
CC involved in mRNA processing via its association with pre-mRNA 3'-end
CC processing factors, establishing a link between pre-tRNA splicing and
CC pre-mRNA 3'-end formation, suggesting that the endonuclease subunits
CC function in multiple RNA-processing events (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC SEN2, SEN15, SEN34/LENG5 and SEN54. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC {ECO:0000305}.
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DR EMBL; AJ720752; CAG32411.1; -; mRNA.
DR RefSeq; NP_001025765.1; NM_001030594.2.
DR AlphaFoldDB; Q5ZIN2; -.
DR STRING; 9031.ENSGALP00000007969; -.
DR PaxDb; Q5ZIN2; -.
DR GeneID; 415965; -.
DR KEGG; gga:415965; -.
DR CTD; 80746; -.
DR VEuPathDB; HostDB:geneid_415965; -.
DR eggNOG; KOG4685; Eukaryota.
DR InParanoid; Q5ZIN2; -.
DR OrthoDB; 1219366at2759; -.
DR PhylomeDB; Q5ZIN2; -.
DR PRO; PR:Q5ZIN2; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006678; tRNA_intron_Endonuc_N.
DR InterPro; IPR006676; tRNA_splic.
DR InterPro; IPR016589; tRNA_splic_SEN2.
DR PANTHER; PTHR21227; PTHR21227; 1.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR Pfam; PF02778; tRNA_int_endo_N; 1.
DR PIRSF; PIRSF011789; tRNA_splic_SEN2; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
PE 2: Evidence at transcript level;
KW Lyase; mRNA processing; Nucleus; Reference proteome; tRNA processing.
FT CHAIN 1..461
FT /note="tRNA-splicing endonuclease subunit Sen2"
FT /id="PRO_0000109455"
FT REGION 140..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 365
FT /evidence="ECO:0000250"
FT ACT_SITE 373
FT /evidence="ECO:0000250"
FT ACT_SITE 412
FT /evidence="ECO:0000250"
SQ SEQUENCE 461 AA; 52134 MW; 69108F9DAE6EC982 CRC64;
MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE DMDQLYNKGY
FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ QRVQWAKSAL QEQGFDGCSV
TKILENYTRP LEFPFLEKGG AEQTGDSCDT VCPNTENTEL SGQSSTDTGN IATSSPECQK
EVCQKAYAEG DPASDSMVGS KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM
AKSRERAPEY VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA
FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW VPKVGLKYGT
DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL SGLNRTTVNA SKELLLCYLI
KPSDMTEEEM ATPECLKRIK VQELIVTRWV SSRERSEQDD L
