SEN34_SCHPO
ID SEN34_SCHPO Reviewed; 284 AA.
AC O60156;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable tRNA-splicing endonuclease subunit sen34;
DE EC=4.6.1.16;
DE AltName: Full=tRNA-intron endonuclease sen34;
GN Name=sen34; ORFNames=SPBC19C7.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC splicing endonuclease complex, a complex responsible for identification
CC and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC 5'-OH termini. There are no conserved sequences at the splice sites,
CC but the intron is invariably located at the same site in the gene,
CC placing the splice sites an invariant distance from the constant
CC structural features of the tRNA body. It probably carries the active
CC site for 3'-splice site cleavage (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC -!- SUBUNIT: Heterotetramer composed of sen2, sen15, sen34 and sen54.
CC Interacts directly with sen15 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA19575.1; -; Genomic_DNA.
DR PIR; T39813; T39813.
DR RefSeq; NP_596163.1; NM_001022083.2.
DR AlphaFoldDB; O60156; -.
DR BioGRID; 277117; 1.
DR STRING; 4896.SPBC19C7.07c.1; -.
DR MaxQB; O60156; -.
DR PaxDb; O60156; -.
DR EnsemblFungi; SPBC19C7.07c.1; SPBC19C7.07c.1:pep; SPBC19C7.07c.
DR GeneID; 2540591; -.
DR KEGG; spo:SPBC19C7.07c; -.
DR PomBase; SPBC19C7.07c; sen34.
DR VEuPathDB; FungiDB:SPBC19C7.07c; -.
DR eggNOG; KOG4133; Eukaryota.
DR HOGENOM; CLU_049366_0_0_1; -.
DR InParanoid; O60156; -.
DR OMA; FIAYPGD; -.
DR PhylomeDB; O60156; -.
DR PRO; PR:O60156; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005741; C:mitochondrial outer membrane; ISO:PomBase.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; ISO:PomBase.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; ISO:PomBase.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; ISO:PomBase.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006676; tRNA_splic.
DR InterPro; IPR016690; tRNA_splic_SEN34.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR PIRSF; PIRSF017250; tRNA_splic_SEN34; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
DR TIGRFAMs; TIGR00324; endA; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome; tRNA processing.
FT CHAIN 1..284
FT /note="Probable tRNA-splicing endonuclease subunit sen34"
FT /id="PRO_0000109467"
FT ACT_SITE 206
FT /evidence="ECO:0000250"
FT ACT_SITE 214
FT /evidence="ECO:0000250"
FT ACT_SITE 245
FT /evidence="ECO:0000250"
SQ SEQUENCE 284 AA; 32808 MW; F40AFEBE0C50F567 CRC64;
MEDEKFPISY VHGKFLVFDV QAVEVFRKNY HILGTLVGTL PQLPQQNVFL GLPMELSKEE
AFYLIEKGIS YIVDDTKVHK QLLENTTKDD VKQCLKKRQS LAYDQMIAAK KKENEKKIEI
MKKLGRTLPL DPLNYDEHDS FDLSWIPVDT VTTRIAEKSS MNDDFHKEED VFENLDINRY
LMFKSLVDTG FYLNPGLRFG CQFVAYPGDA LRYHSHYLVN SYKWDQEIPV LFLIGGGRLG
TAVKKTWLIG GSNDRNINMN GEKSKEELLL LPVRHFSIEW AGFG
