ID   SEN54_ASHGO             Reviewed;         455 AA.
AC   Q74ZJ5;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=tRNA-splicing endonuclease subunit SEN54;
DE   AltName: Full=tRNA-intron endonuclease SEN54;
GN   Name=SEN54; OrderedLocusNames=AGR205C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Non-catalytic subunit of the tRNA-splicing endonuclease
CC       complex, a complex responsible for identification and cleavage of the
CC       splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice
CC       sites to release the intron. The products are an intron and two tRNA
CC       half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There
CC       are no conserved sequences at the splice sites, but the intron is
CC       invariably located at the same site in the gene, placing the splice
CC       sites an invariant distance from the constant structural features of
CC       the tRNA body. May be required to embody the molecular ruler of the
CC       complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC       SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN2 (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SEN54 family. {ECO:0000305}.
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DR   EMBL; AE016820; AAS54695.1; -; Genomic_DNA.
DR   RefSeq; NP_986871.1; NM_211933.1.
DR   AlphaFoldDB; Q74ZJ5; -.
DR   STRING; 33169.AAS54695; -.
DR   EnsemblFungi; AAS54695; AAS54695; AGOS_AGR205C.
DR   GeneID; 4623173; -.
DR   KEGG; ago:AGOS_AGR205C; -.
DR   eggNOG; KOG4772; Eukaryota.
DR   HOGENOM; CLU_028449_0_1_1; -.
DR   InParanoid; Q74ZJ5; -.
DR   OMA; FNVWKPQ; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:EnsemblFungi.
DR   GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IEA:EnsemblFungi.
DR   GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR   InterPro; IPR024337; tRNA_splic_suSen54.
DR   InterPro; IPR024336; tRNA_splic_suSen54_N.
DR   PANTHER; PTHR21027; PTHR21027; 1.
DR   Pfam; PF12928; tRNA_int_end_N2; 1.
PE   3: Inferred from homology;
KW   Reference proteome; tRNA processing.
FT   CHAIN           1..455
FT                   /note="tRNA-splicing endonuclease subunit SEN54"
FT                   /id="PRO_0000194031"
FT   REGION          366..390
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          434..455
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        434..448
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   455 AA;  52261 MW;  938D0CD59281171C CRC64;
     MTEAEELSVA LGLHDSDAEE DALAQDWSEM AKLVKKANQH ALPRRGEKDY EPDGTDAQAL
     LLHTAKETMF GTLLNSVRGS TVKTLIKAYW EEEQRMARIP NARGSFTNTM GKVDKHGQCW
     LQLHEFVYLV ERGTVSPYLA LTVGDEKSNH EDVLLSVQDV YALFSSTEEL DEFLVYAHLK
     RLGFIVVSTD NPPAHVTSFY PPLSQKSSAS SNWFCHIHSW LKTPQNLFHV PLYHPLHFLL
     HRYTSSPQLY EELSQHIPFK KVPHDINELR EERNNGILNP NVKQWKIAFN VWKPKSSFKK
     KTPGLPDFQV VVYNKDNAGQ QFPTYDEFRS IFHRLDYRFD FLNELDVDDS AWDDYTYTDG
     MPTREFMQRN KIAPSQPHSG GDSRKKKART YPQHIAQIRR LKSGFKSFIL SVIDDGLVSF
     VRIAESDFGS SNIWYTPPSQ TGTTNNRTRS DKVDV