SEN54_NEUCR
ID SEN54_NEUCR Reviewed; 557 AA.
AC Q7SC91;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-FEB-2014, sequence version 2.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Probable tRNA-splicing endonuclease subunit tsp-5;
DE AltName: Full=tRNA-intron endonuclease sen54;
GN Name=tsp-5; Synonyms=sen54; ORFNames=B18P7.090, NCU08510;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Non-catalytic subunit of the tRNA-splicing endonuclease
CC complex, a complex responsible for identification and cleavage of the
CC splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice
CC sites to release the intron. The products are an intron and two tRNA
CC half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There
CC are no conserved sequences at the splice sites, but the intron is
CC invariably located at the same site in the gene, placing the splice
CC sites an invariant distance from the constant structural features of
CC the tRNA body. May be required to embody the molecular ruler of the
CC complex (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts
CC directly with tsp-2/sen2 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SEN54 family. {ECO:0000305}.
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DR EMBL; BX842594; CAE75682.1; -; Genomic_DNA.
DR EMBL; CM002237; EAA34203.2; -; Genomic_DNA.
DR RefSeq; XP_963439.2; XM_958346.2.
DR AlphaFoldDB; Q7SC91; -.
DR STRING; 5141.EFNCRP00000008482; -.
DR EnsemblFungi; EAA34203; EAA34203; NCU08510.
DR GeneID; 3879579; -.
DR KEGG; ncr:NCU08510; -.
DR VEuPathDB; FungiDB:NCU08510; -.
DR HOGENOM; CLU_028449_2_0_1; -.
DR InParanoid; Q7SC91; -.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0000214; C:tRNA-intron endonuclease complex; IBA:GO_Central.
DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR InterPro; IPR024337; tRNA_splic_suSen54.
DR InterPro; IPR024336; tRNA_splic_suSen54_N.
DR PANTHER; PTHR21027; PTHR21027; 1.
DR Pfam; PF12928; tRNA_int_end_N2; 1.
PE 3: Inferred from homology;
KW Reference proteome; tRNA processing.
FT CHAIN 1..557
FT /note="Probable tRNA-splicing endonuclease subunit tsp-5"
FT /id="PRO_0000194032"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 370..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 370..397
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 557 AA; 60957 MW; 59F2228B2F49AC24 CRC64;
MPLDDDLEDN PSLVPPPSTT TTSSNATGDA ATMMDEVEEA EDAPTADFRL FASTYSKTKN
ISAQTIRKGE KDFESHGTRA QASALDASRD AMRDVLSYTR VHNTKSASGW CRGWYFPDWW
KDWPEDWEQQ KLTKRGKEGA GEDDEEEKDR KLPPLHVRDR VVLLEHTNVA SQSLGRAVTG
LPKDRPARGR EWLLPEEALY LVERGSLDLW WPTKGIEEVF PADGSVPAAA AATTSAKGEG
EQRTEEDEED DDEYKYGLPL SLQAAYALLI GEDGERGKVS LQKFQVFSHL KRAGYNVIRA
PTNPLPVQED TQLTTTTQPA SKPISVTEWL ISCLPQSKSS PTDPPPYGPL VPPGFYRSYN
TIYNYLSLRP SSTSSSASPT ADNQPQKPQS PESDESDSGS DSPYKIHYHV YKASTKFTRT
RPPPPDFYIS VISAKDTSIP TLSEISSLLA SAPADLPKAE WLAGGPARLY ARLKHGYRNV
LLAVNDHGVI NYMRFAEGGF AKEELFRNYD MRVSGGPRRG GGGGGKKSGN NNGRGSRGRG
GGRGGGRGGR GGRGRGN
