ID   SEN54_SCHPO             Reviewed;         384 AA.
AC   O74908;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Probable tRNA-splicing endonuclease subunit sen54;
DE   AltName: Full=tRNA-intron endonuclease sen54;
GN   Name=sen54; ORFNames=SPCC613.09;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Non-catalytic subunit of the tRNA-splicing endonuclease
CC       complex, a complex responsible for identification and cleavage of the
CC       splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice
CC       sites to release the intron. The products are an intron and two tRNA
CC       half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There
CC       are no conserved sequences at the splice sites, but the intron is
CC       invariably located at the same site in the gene, placing the splice
CC       sites an invariant distance from the constant structural features of
CC       the tRNA body. May be required to embody the molecular ruler of the
CC       complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC       sen2, sen15, sen34 and sen54. Interacts directly with sen2 (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SEN54 family. {ECO:0000305}.
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DR   EMBL; CU329672; CAA21061.1; -; Genomic_DNA.
DR   PIR; T41475; T41475.
DR   RefSeq; NP_587697.1; NM_001022692.2.
DR   AlphaFoldDB; O74908; -.
DR   STRING; 4896.SPCC613.09.1; -.
DR   MaxQB; O74908; -.
DR   PaxDb; O74908; -.
DR   PRIDE; O74908; -.
DR   EnsemblFungi; SPCC613.09.1; SPCC613.09.1:pep; SPCC613.09.
DR   GeneID; 2538979; -.
DR   KEGG; spo:SPCC613.09; -.
DR   PomBase; SPCC613.09; sen54.
DR   VEuPathDB; FungiDB:SPCC613.09; -.
DR   eggNOG; KOG4772; Eukaryota.
DR   HOGENOM; CLU_028449_0_1_1; -.
DR   InParanoid; O74908; -.
DR   OMA; MYMRLRH; -.
DR   PhylomeDB; O74908; -.
DR   PRO; PR:O74908; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0031966; C:mitochondrial membrane; HDA:PomBase.
DR   GO; GO:0005741; C:mitochondrial outer membrane; ISO:PomBase.
DR   GO; GO:0000214; C:tRNA-intron endonuclease complex; ISO:PomBase.
DR   GO; GO:0090502; P:RNA phosphodiester bond hydrolysis, endonucleolytic; IEA:GOC.
DR   GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; ISO:PomBase.
DR   InterPro; IPR024337; tRNA_splic_suSen54.
DR   InterPro; IPR024336; tRNA_splic_suSen54_N.
DR   PANTHER; PTHR21027; PTHR21027; 1.
DR   Pfam; PF12928; tRNA_int_end_N2; 1.
PE   3: Inferred from homology;
KW   Reference proteome; tRNA processing.
FT   CHAIN           1..384
FT                   /note="Probable tRNA-splicing endonuclease subunit sen54"
FT                   /id="PRO_0000194033"
FT   REGION          37..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   384 AA;  44151 MW;  69AA96FD3AF30636 CRC64;
     MDAQDEDNPF TNLETTVDVT EEIQDWRFLS NVEKDQGTYT IPKRGQKDFE PDGTNKQHSA
     LDLSRKAMFD ALSVERLISA KHAIIATWNA QNGMSCVEKA HGPLFKTMGT ADSQNRMWLL
     PEETLYLVER GSMECWSEEG LPMSLQAVYS ASIPLCGSLE NYLVYAHLRR CGFSVIRSNL
     VPVKEDEYRC DSKIMNFKDL LFLGLGKASQ ILQTFNFRKL AFPFSKRRRQ SILLHDTFYT
     YEEVYHDLQI VRGYVPIACN LITSSDSLFQ ITFHAYKPSA SFKKSALSEP DFRICVVSSQ
     DTLLPTIFEI DALFSSTPLR QNMPQHMFQR LKEGYRNIII AIVDYGVISY IRLSDVCFEE
     KVYTDFSKKG SKRKRVSKKF QQLV