SENJU_DROME
ID SENJU_DROME Reviewed; 388 AA.
AC Q9VMU8; Q8MYZ6;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=UDP-galactose transporter senju {ECO:0000303|PubMed:25901322};
GN Name=senju {ECO:0000312|FlyBase:FBgn0031676};
GN ORFNames=CG14040 {ECO:0000312|FlyBase:FBgn0031676};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM29464.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM29464.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM29464.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=25901322; DOI=10.1073/pnas.1424514112;
RA Yamamoto-Hino M., Muraoka M., Kondo S., Ueda R., Okano H., Goto S.;
RT "Dynamic regulation of innate immune responses in Drosophila by Senju-
RT mediated glycosylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:5809-5814(2015).
CC -!- FUNCTION: UDP-galactose transporter involved in the synthesis of
CC galactose-containing glycans. Plays a role in quiescence of the innate
CC immune response, possibly by regulating glycosylation of the Toll
CC pathway ligand spz. {ECO:0000269|PubMed:25901322}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25901322}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Detected in lymph glands from late third-instar
CC larvae (at protein level). {ECO:0000269|PubMed:25901322}.
CC -!- DISRUPTION PHENOTYPE: Lethal in late larvae and early pupal stages. In
CC immunologically unchallenged third-instar larvae, displays
CC abnormalities of the immune organs, including melanotic cells in the
CC hemocoel and melanotic tumors in the lymph glands; in the primary and
CC secondary lobes, shows activation of lamellocytes and reduced numbers
CC of hematocyte progenitor cells and plasmatocytes. In addition, displays
CC hyperactivation of immune signal transduction via the Toll, JAK/STAT
CC and JNK pathways; shows reduced expression of galactose-containing
CC glycans affecting also spz glycosylation.
CC {ECO:0000269|PubMed:25901322}.
CC -!- MISCELLANEOUS: The gene name means 'Buddha with a thousand hands
CC manipulating tools to protect us' in Japanese.
CC {ECO:0000303|PubMed:25901322}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM29464.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF52211.2; -; Genomic_DNA.
DR EMBL; AY113459; AAM29464.1; ALT_FRAME; mRNA.
DR RefSeq; NP_608902.1; NM_135058.4.
DR AlphaFoldDB; Q9VMU8; -.
DR SMR; Q9VMU8; -.
DR STRING; 7227.FBpp0078648; -.
DR PaxDb; Q9VMU8; -.
DR PRIDE; Q9VMU8; -.
DR EnsemblMetazoa; FBtr0079009; FBpp0078648; FBgn0031676.
DR GeneID; 33734; -.
DR KEGG; dme:Dmel_CG14040; -.
DR UCSC; CG14040-RA; d. melanogaster.
DR CTD; 33734; -.
DR FlyBase; FBgn0031676; senju.
DR VEuPathDB; VectorBase:FBgn0031676; -.
DR eggNOG; KOG2234; Eukaryota.
DR GeneTree; ENSGT00950000182827; -.
DR HOGENOM; CLU_024645_5_0_1; -.
DR InParanoid; Q9VMU8; -.
DR OMA; CLYCREN; -.
DR OrthoDB; 754910at2759; -.
DR PhylomeDB; Q9VMU8; -.
DR BioGRID-ORCS; 33734; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 33734; -.
DR PRO; PR:Q9VMU8; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031676; Expressed in oocyte and 26 other tissues.
DR Genevisible; Q9VMU8; DM.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0005797; C:Golgi medial cisterna; IDA:FlyBase.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0045824; P:negative regulation of innate immune response; IMP:FlyBase.
DR GO; GO:0045751; P:negative regulation of Toll signaling pathway; IMP:FlyBase.
DR GO; GO:0002225; P:positive regulation of antimicrobial peptide production; IGI:UniProtKB.
DR GO; GO:0060050; P:positive regulation of protein glycosylation; IMP:UniProtKB.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IDA:FlyBase.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..388
FT /note="UDP-galactose transporter senju"
FT /evidence="ECO:0000305"
FT /id="PRO_0000441162"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 125
FT /note="T -> A (in Ref. 3; AAM29464)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 388 AA; 43616 MW; 9495C398C3263413 CRC64;
MSTNWRELFP TKLTFVIFLL YMSLFIGQGI FVTASQESNN SYGYNTVTVV LLTEVFKLIV
STCLYCRDNN LRSLVRDVQK DRNVLGLYMV PAFLYCLYNN LAFVNLATFD PTTYYLLLQL
RVVVTGILFQ IIFKKYLSQR QWISLILLTL GCMMKQVDFG SFYSDANDDS ESAAIQQQLQ
SHNKTTSAET HAHGKNMSGF DFSLSAVFIL AQTICSCLAG VYNEYLLKDK GADVNIFVQN
VFMYLDSIVC NAVILLLRGE LLDAFSPQNL GSIMRFSVLI IIVNNAAIGI VTSFFLKYMN
SILKTFASAL ELLFTAVLCY FLFSIPIYMN TALAIAVVSY AIYLYTQSPV VNLGKVRPLS
NLSDATTKST DKRKLIDEEA AESDLDMV
