ID   SENP8_DICDI             Reviewed;         243 AA.
AC   Q54XR2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Probable sentrin-specific protease 8;
DE            EC=3.4.22.-;
DE   AltName: Full=Deneddylase;
DE   AltName: Full=Sentrin/sumo-specific protease senp8;
GN   Name=senp8; ORFNames=DDB_G0278795;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Protease that catalyzes two essential functions in the nedd8
CC       pathway: processing of full-length nedd8 to its mature form and
CC       deconjugation of nedd8 from targeted proteins. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C48 family. {ECO:0000305}.
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DR   EMBL; AAFI02000024; EAL67999.1; -; Genomic_DNA.
DR   RefSeq; XP_641968.1; XM_636876.1.
DR   AlphaFoldDB; Q54XR2; -.
DR   SMR; Q54XR2; -.
DR   STRING; 44689.DDB0304995; -.
DR   PaxDb; Q54XR2; -.
DR   EnsemblProtists; EAL67999; EAL67999; DDB_G0278795.
DR   GeneID; 8621700; -.
DR   KEGG; ddi:DDB_G0278795; -.
DR   dictyBase; DDB_G0278795; senp8.
DR   eggNOG; KOG3246; Eukaryota.
DR   HOGENOM; CLU_043678_3_1_1; -.
DR   InParanoid; Q54XR2; -.
DR   OMA; LLDPPNW; -.
DR   PhylomeDB; Q54XR2; -.
DR   PRO; PR:Q54XR2; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019784; F:deNEDDylase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR044613; Nep1/2-like.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR003653; Peptidase_C48_C.
DR   PANTHER; PTHR46468; PTHR46468; 1.
DR   Pfam; PF02902; Peptidase_C48; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS50600; ULP_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease;
KW   Ubl conjugation pathway.
FT   CHAIN           1..243
FT                   /note="Probable sentrin-specific protease 8"
FT                   /id="PRO_0000328419"
FT   REGION          12..185
FT                   /note="Protease"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        109
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        126
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        174
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   243 AA;  28095 MW;  BA25465FB693F506 CRC64;
     MSDPLIVSYN DSAIYQSDIN ILKSNQWLND SIISFYLEWL KDGGEDNKNK IPNQVLLLSP
     SVVFCCSFVE SEQEIQLMFE QPLSLKTKEV IFFPLTNNRD PNVIGGGTHW SLLIFIKSLN
     KFIYYDSINS FNSSDAIFII SKFKFLLSSP PPKTNLKEFL INQKTPQQQN GYDCGLYVLS
     IIEELLKLII KENENNKGEE NKISYKDLLL SEFTNELLFK EITPNYIKDK RVEILNTILK
     LKK