SENP8_MOUSE
ID SENP8_MOUSE Reviewed; 221 AA.
AC Q9D2Z4; Q543P0;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2002, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Sentrin-specific protease 8;
DE EC=3.4.22.-;
DE AltName: Full=Deneddylase-1;
DE AltName: Full=NEDD8-specific protease 1;
DE AltName: Full=Sentrin/SUMO-specific protease SENP8;
GN Name=Senp8; Synonyms=Den1, Nedp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cecum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND FUNCTION.
RX PubMed=12759362; DOI=10.1074/jbc.m302890200;
RA Gan-Erdene T., Nagamalleswari K., Yin L., Wu K., Pan Z.-Q., Wilkinson K.D.;
RT "Identification and characterization of DEN1, a deneddylase of the ULP
RT family.";
RL J. Biol. Chem. 278:28892-28900(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14673145; DOI=10.1128/mcb.24.1.84-95.2004;
RA Hemelaar J., Borodovsky A., Kessler B.M., Reverter D., Cook J., Kolli N.,
RA Gan-Erdene T., Wilkinson K.D., Gill G., Lima C.D., Ploegh H.L., Ovaa H.;
RT "Specific and covalent targeting of conjugating and deconjugating enzymes
RT of ubiquitin-like proteins.";
RL Mol. Cell. Biol. 24:84-95(2004).
CC -!- FUNCTION: Protease that catalyzes two essential functions in the NEDD8
CC pathway: processing of full-length NEDD8 to its mature form and
CC deconjugation of NEDD8 from targeted proteins such as cullins or p53.
CC {ECO:0000269|PubMed:12759362}.
CC -!- SIMILARITY: Belongs to the peptidase C48 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB31304.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK018606; BAB31304.1; ALT_INIT; mRNA.
DR EMBL; AK049120; BAC33554.1; -; mRNA.
DR EMBL; BC037443; AAH37443.1; -; mRNA.
DR CCDS; CCDS23254.1; -.
DR RefSeq; NP_001165539.1; NM_001172068.1.
DR RefSeq; NP_001165540.1; NM_001172069.1.
DR RefSeq; NP_001165541.1; NM_001172070.1.
DR RefSeq; NP_001165542.1; NM_001172071.1.
DR RefSeq; NP_082114.1; NM_027838.3.
DR AlphaFoldDB; Q9D2Z4; -.
DR SMR; Q9D2Z4; -.
DR BioGRID; 214805; 1.
DR STRING; 10090.ENSMUSP00000129441; -.
DR MEROPS; C48.011; -.
DR PhosphoSitePlus; Q9D2Z4; -.
DR MaxQB; Q9D2Z4; -.
DR PaxDb; Q9D2Z4; -.
DR PRIDE; Q9D2Z4; -.
DR ProteomicsDB; 256961; -.
DR Antibodypedia; 26582; 298 antibodies from 29 providers.
DR DNASU; 71599; -.
DR Ensembl; ENSMUST00000051039; ENSMUSP00000054509; ENSMUSG00000051705.
DR Ensembl; ENSMUST00000163586; ENSMUSP00000129441; ENSMUSG00000051705.
DR Ensembl; ENSMUST00000177963; ENSMUSP00000137228; ENSMUSG00000051705.
DR Ensembl; ENSMUST00000217093; ENSMUSP00000150601; ENSMUSG00000051705.
DR GeneID; 71599; -.
DR KEGG; mmu:71599; -.
DR UCSC; uc009pym.2; mouse.
DR CTD; 123228; -.
DR MGI; MGI:1918849; Senp8.
DR VEuPathDB; HostDB:ENSMUSG00000051705; -.
DR eggNOG; KOG3246; Eukaryota.
DR GeneTree; ENSGT00390000014038; -.
DR HOGENOM; CLU_043678_3_1_1; -.
DR InParanoid; Q9D2Z4; -.
DR OMA; LLDPPNW; -.
DR OrthoDB; 1313098at2759; -.
DR PhylomeDB; Q9D2Z4; -.
DR TreeFam; TF351057; -.
DR BioGRID-ORCS; 71599; 8 hits in 70 CRISPR screens.
DR PRO; PR:Q9D2Z4; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9D2Z4; protein.
DR Bgee; ENSMUSG00000051705; Expressed in secondary oocyte and 219 other tissues.
DR ExpressionAtlas; Q9D2Z4; baseline and differential.
DR Genevisible; Q9D2Z4; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008234; F:cysteine-type peptidase activity; ISO:MGI.
DR GO; GO:0019784; F:deNEDDylase activity; ISO:MGI.
DR GO; GO:0016929; F:deSUMOylase activity; IBA:GO_Central.
DR GO; GO:0016926; P:protein desumoylation; IBA:GO_Central.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR003653; Peptidase_C48_C.
DR Pfam; PF02902; Peptidase_C48; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS50600; ULP_PROTEASE; 1.
PE 1: Evidence at protein level;
KW Acetylation; Hydrolase; Protease; Reference proteome; Thiol protease;
KW Ubl conjugation pathway.
FT CHAIN 1..221
FT /note="Sentrin-specific protease 8"
FT /id="PRO_0000101728"
FT REGION 11..174
FT /note="Protease"
FT ACT_SITE 102
FT /evidence="ECO:0000250"
FT ACT_SITE 119
FT /evidence="ECO:0000250"
FT ACT_SITE 163
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q96LD8"
SQ SEQUENCE 221 AA; 25057 MW; AF9EB91D8A1C6F39 CRC64;
MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VCFISPEVTQ
FIKCTSSPAE IAMFLEPLDL PHKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS
HSRSNSIHAK QVAEKLKAFL GSKGDKLVFV EEKAPAQENS YDCGMYVICN TEALCQSLFR
RQPESPLQLL TPTYITKKRG EWKDLIARLA KKNEVATEEC S
