ID   BGIA_MOMCH              Reviewed;          68 AA.
AC   P24076;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Glu S.griseus protease inhibitor;
DE   AltName: Full=BGIA;
OS   Momordica charantia (Bitter gourd) (Balsam pear).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3673;
RN   [1]
RP   PROTEIN SEQUENCE, AND ACETYLATION AT SER-1.
RC   TISSUE=Seed;
RX   PubMed=1679433; DOI=10.1016/s0021-9258(18)55360-6;
RA   Ogata F., Miyata T., Fujii N., Yoshida N., Noda K., Makisumi S., Ito A.;
RT   "Purification and amino acid sequence of a bitter gourd inhibitor against
RT   an acidic amino acid-specific endopeptidase of Streptomyces griseus.";
RL   J. Biol. Chem. 266:16715-16721(1991).
CC   -!- FUNCTION: Competitively inhibits Glu S.griseus protease by forming
CC       probably a 1:1 complex. BGIA has no inhibitory activity against 2 other
CC       acidic amino acid-specific endopeptidases (S.aureus protease V8 and
CC       B.subtilis proteinase), chymotrypsin, trypsin, pancreatic elastase, and
CC       papain, although subtilisin Carlsberg was strongly inhibited.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   PIR; A41174; A41174.
DR   AlphaFoldDB; P24076; -.
DR   SMR; P24076; -.
DR   MEROPS; I13.004; -.
DR   iPTMnet; P24076; -.
DR   Proteomes; UP000504603; Unplaced.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   PRINTS; PR00292; POTATOINHBTR.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Reference proteome; Serine protease inhibitor.
FT   CHAIN           1..68
FT                   /note="Glu S.griseus protease inhibitor"
FT                   /id="PRO_0000217644"
FT   SITE            44..45
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         1
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000269|PubMed:1679433"
FT   DISULFID        3..48
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   68 AA;  7383 MW;  97C98BF4AD71E933 CRC64;
     SQCQGKRSWP QLVGSTGAAA KAVIERENPR VRAVIVRVGS PVTADFRCDR VRVWVTERGI
     VARPPAIG