ID   SEP2_ARATH              Reviewed;         250 AA.
AC   P29384;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 158.
DE   RecName: Full=Developmental protein SEPALLATA 2;
DE   AltName: Full=Agamous-like MADS-box protein AGL4;
GN   Name=SEP2; Synonyms=AGL4; OrderedLocusNames=At3g02310; ORFNames=F14P3.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=1672119; DOI=10.1101/gad.5.3.484;
RA   Ma H., Yanofsky M.F., Meyerowitz E.M.;
RT   "AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic
RT   and transcription factor genes.";
RL   Genes Dev. 5:484-495(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   CHARACTERIZATION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX   PubMed=9418042; DOI=10.1046/j.1365-313x.1997.12050999.x;
RA   Fan H.-Y., Hu Y., Tudor M., Ma H.;
RT   "Specific interactions between the K domains of AG and AGLs, members of the
RT   MADS domain family of DNA binding proteins.";
RL   Plant J. 12:999-1010(1997).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=10821278; DOI=10.1038/35012103;
RA   Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.;
RT   "B and C floral organ identity functions require SEPALLATA MADS-box
RT   genes.";
RL   Nature 405:200-203(2000).
RN   [6]
RP   INTERACTION WITH TT16/AGL32.
RX   PubMed=16080001; DOI=10.1007/s00438-005-0010-y;
RA   Kaufmann K., Anfang N., Saedler H., Theissen G.;
RT   "Mutant analysis, protein-protein interactions and subcellular localization
RT   of the Arabidopsis B sister (ABS) protein.";
RL   Mol. Genet. Genomics 274:103-118(2005).
CC   -!- FUNCTION: Probable transcription factor. Functions with SEPALLATA1/AGL2
CC       and SEPALLATA3/AGL9 to ensure proper development of petals, stamens and
CC       carpels and to prevent the indeterminate growth of the flower meristem.
CC       Forms a heterodimer via the K-box domain with AG, that could be
CC       involved in genes regulation during floral meristem development.
CC   -!- SUBUNIT: Heterodimer with AGAMOUS capable of binding to CArG-box
CC       sequences (PubMed:9418042). Interacts with TT16/AGL32
CC       (PubMed:16080001). {ECO:0000269|PubMed:16080001,
CC       ECO:0000269|PubMed:9418042}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DEVELOPMENTAL STAGE: Expressed early during flower development.
CC   -!- DISRUPTION PHENOTYPE: Triple mutations in the SEP1, SEP2 and SEP3 genes
CC       result in the replacement of the stamens and petals by sepals and of
CC       the carpels by a new mutant flower with sepaloid organs.
CC       {ECO:0000269|PubMed:9418042}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M55552; AAA32734.1; -; mRNA.
DR   EMBL; AC009755; AAF02125.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE73791.1; -; Genomic_DNA.
DR   PIR; D39534; D39534.
DR   RefSeq; NP_186880.1; NM_111098.4.
DR   AlphaFoldDB; P29384; -.
DR   SMR; P29384; -.
DR   BioGRID; 6484; 14.
DR   DIP; DIP-33790N; -.
DR   IntAct; P29384; 15.
DR   STRING; 3702.AT3G02310.1; -.
DR   PaxDb; P29384; -.
DR   PRIDE; P29384; -.
DR   ProteomicsDB; 232784; -.
DR   EnsemblPlants; AT3G02310.1; AT3G02310.1; AT3G02310.
DR   GeneID; 821151; -.
DR   Gramene; AT3G02310.1; AT3G02310.1; AT3G02310.
DR   KEGG; ath:AT3G02310; -.
DR   Araport; AT3G02310; -.
DR   TAIR; locus:2076522; AT3G02310.
DR   eggNOG; KOG0014; Eukaryota.
DR   HOGENOM; CLU_053053_0_2_1; -.
DR   InParanoid; P29384; -.
DR   OrthoDB; 1039681at2759; -.
DR   PhylomeDB; P29384; -.
DR   PRO; PR:P29384; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; P29384; baseline and differential.
DR   Genevisible; P29384; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..250
FT                   /note="Developmental protein SEPALLATA 2"
FT                   /id="PRO_0000199484"
FT   DOMAIN          3..57
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          88..178
FT                   /note="K-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT   COILED          85..150
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   250 AA;  28578 MW;  53136FE263389943 CRC64;
     MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVSLIVFSN RGKLYEFCST
     SNMLKTLERY QKCSYGSIEV NNKPAKELEN SYREYLKLKG RYENLQRQQR NLLGEDLGPL
     NSKELEQLER QLDGSLKQVR CIKTQYMLDQ LSDLQGKEHI LLDANRALSM KLEDMIGVRH
     HHIGGGWEGG DQQNIAYGHP QAHSQGLYQS LECDPTLQIG YSHPVCSEQM AVTVQGQSQQ
     GNGYIPGWML