ID   SEPA_PSEP1              Reviewed;         382 AA.
AC   Q849Q9; A5W4D8;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Probable efflux pump periplasmic linker protein SepA;
DE   Flags: Precursor;
GN   Name=sepA; OrderedLocusNames=Pput_2867;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9037074; DOI=10.1073/pnas.94.4.1453;
RA   Lau P.C., Wang Y., Patel A., Labbe D., Bergeron H., Brousseau R.,
RA   Konishi Y., Rawlings M.;
RT   "A bacterial basic region leucine zipper histidine kinase regulating
RT   toluene degradation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:1453-1458(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable periplasmic linker protein component of the SepABC
CC       efflux pump with unknown specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABQ78998.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U72354; AAO49780.1; -; Genomic_DNA.
DR   EMBL; CP000712; ABQ78998.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q849Q9; -.
DR   SMR; Q849Q9; -.
DR   STRING; 351746.Pput_2867; -.
DR   EnsemblBacteria; ABQ78998; ABQ78998; Pput_2867.
DR   KEGG; ppf:Pput_2867; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_018816_2_1_6; -.
DR   OMA; ARINLNW; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Coiled coil; Lipoprotein; Membrane;
KW   Palmitate; Signal; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..382
FT                   /note="Probable efflux pump periplasmic linker protein
FT                   SepA"
FT                   /id="PRO_0000018714"
FT   COILED          100..136
FT                   /evidence="ECO:0000255"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   382 AA;  41558 MW;  F0DD9A1979592757 CRC64;
     MRLERALRAR QLIPLAAIWL LVGCGKQETV ESTAVPPEVG VYTVKAQALT LTTDLPGRTS
     AYRVSEVRPQ ASGILQKRMF VEGAEVKQGE QLYQIDPRTY EALLARAEAS LLTAQNLARR
     YERLLDTNAI SQQQYDDAMA TWKQAQAEAQ MARINMQYTK VLAPITGRIG RSAVTEGALV
     TNGQAQELAT VTQLDPIYVD VNQPITRLLG LKRALESGRL QRVGDNQAQV SLTLDDGTPY
     PLKGVLKFSE VSVAPSTGSV TLRAEFPNPD HKLLPGMFVH ALLNEGEQQA AILVPHQAVG
     RDARGVPTVW VVKPDNTVES REVQTLQTVG NAWLLGAGIN DGERVITEGV QLARSGITVK
     PVAAKNVKLM SEFGSQVQAQ AH