ID   SEPB_PSEP1              Reviewed;        1046 AA.
AC   Q849R0; A5W4D9;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Probable efflux pump membrane transporter SepB;
GN   Name=sepB; OrderedLocusNames=Pput_2868;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9037074; DOI=10.1073/pnas.94.4.1453;
RA   Lau P.C., Wang Y., Patel A., Labbe D., Bergeron H., Brousseau R.,
RA   Konishi Y., Rawlings M.;
RT   "A bacterial basic region leucine zipper histidine kinase regulating
RT   toluene degradation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:1453-1458(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable membrane transporter component of the SepABC efflux
CC       pump with unknown specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
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DR   EMBL; U72354; AAO49779.1; -; Genomic_DNA.
DR   EMBL; CP000712; ABQ78999.1; -; Genomic_DNA.
DR   RefSeq; WP_012052588.1; NC_009512.1.
DR   AlphaFoldDB; Q849R0; -.
DR   SMR; Q849R0; -.
DR   STRING; 351746.Pput_2868; -.
DR   EnsemblBacteria; ABQ78999; ABQ78999; Pput_2868.
DR   KEGG; ppf:Pput_2868; -.
DR   eggNOG; COG0841; Bacteria.
DR   HOGENOM; CLU_002755_0_1_6; -.
DR   OMA; PAIMEMG; -.
DR   OrthoDB; 91817at2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004764; HAE1.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00915; 2A0602; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..1046
FT                   /note="Probable efflux pump membrane transporter SepB"
FT                   /id="PRO_0000161853"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        370..390
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        470..490
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        542..562
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        895..915
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        927..947
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        973..993
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1008..1028
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1046 AA;  113360 MW;  09C97337E6DA8904 CRC64;
     MSRFFIDRPI FAWVLAIIAM LAGALSLTKM PISQYPNIAA PAVSIQVVYP GASAKTVQDT
     VVQVIEQQLN GLDGFRYMAA ESASDGSMNI IVTFEQGTNP DIAQVQVQNK LQLATPRLPE
     EVQRQGLRVV KYQMNFFMVV GLVDKTGKMT NFDLGNLIAS QLQDPISRIN GVGDFLLFGS
     PYAMRIWLDP GKLNSYQLTP GDVAQAIREQ NVQVSSGQLG GLPTRSGVQL NATVVGKTRM
     TTPAEFEEIL VKVKADGSQV RVKDLGRVVL ASENFAISAK YRGQDSAGLG LRLASGGNLL
     ETVKAVKAEL EKQKAYLPEG VEVIYPYDTS PVVEASIDSV VHTILEAVVL VFLVMFLFLQ
     SLRATIIPTL AVPVVLLAAF ALLPYFGISI NVLTMYAMVL AIGLLVDDAI VVVENVERLM
     HDEGLSPLEA TRKSMGQISG ALVGIGMVLS AVFVPMAFFG GSAGIIYKQF AVTIVICMSL
     SVLVALIFTP ALCATILKAP ENDAHHEKKG FFGWFNRSFD RNSARFERGV GGILKHRGRY
     LLIFALITAG TGYLFTQIPK AFLPSEDQGL MMTEVRMPLN ASAERTEVVL QEVKDYLLKE
     EGQLVDHVMT VNGFNFAGRG QNSGLVLVVL KDWAARQAAG EDVLSVAERA NARFARIKDA
     TVMAFVPPAV LEMGNAMGFD LYLQDNLGLG HESLMAARNQ FLELAAENPS LRAVRPNGKD
     DEPQFQVKID DEKARALQVS IASINDTMSA AWGSMYVNDF IDLGRVKRVY IQGVDSSRIA
     PEDFDKWYVR NALGEMVPFS AFATGEWIHG SPKLERYGGI SAVNILGEPA PGFSTGDAMI
     AIAQIMQQLP SGIGLSYNGL SYEEIRTGDQ APMLYALTVL IVFLCLAALY ESWSVPMSVI
     LVVPLGIFGA VLATLWRGLE ADVYFQVGLM TTVGLSAKNA ILIIEFAKEL YEKEGVPLVK
     AAIEAARLRL RPIIMTSLAF TFGVLPMARA TGAGAGSQHS IATGVVGGMI TATVLAVFFV
     PLFYVVVVKV FERNKKPAAL AEEELA