BGL02_ARATH
ID BGL02_ARATH Reviewed; 299 AA.
AC Q9FMD8;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Putative beta-glucosidase 2;
DE Short=AtBGLU2;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU2; OrderedLocusNames=At5g16580; ORFNames=MTG13.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15604686; DOI=10.1007/s11103-004-0790-1;
RA Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R.,
RA Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 1.";
RL Plant Mol. Biol. 55:343-367(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10185.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB008270; BAB10185.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92313.1; -; Genomic_DNA.
DR RefSeq; NP_197161.2; NM_121664.3.
DR AlphaFoldDB; Q9FMD8; -.
DR SMR; Q9FMD8; -.
DR STRING; 3702.AT5G16580.1; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q9FMD8; -.
DR PRIDE; Q9FMD8; -.
DR EnsemblPlants; AT5G16580.1; AT5G16580.1; AT5G16580.
DR GeneID; 831520; -.
DR Gramene; AT5G16580.1; AT5G16580.1; AT5G16580.
DR KEGG; ath:AT5G16580; -.
DR Araport; AT5G16580; -.
DR TAIR; locus:2174180; AT5G16580.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_4_0_1; -.
DR InParanoid; Q9FMD8; -.
DR OMA; FPWAMES; -.
DR OrthoDB; 408001at2759; -.
DR PhylomeDB; Q9FMD8; -.
DR BioCyc; ARA:AT5G16580-MON; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMD8; baseline and differential.
DR Genevisible; Q9FMD8; AT.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 5: Uncertain;
KW Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..299
FT /note="Putative beta-glucosidase 2"
FT /id="PRO_0000389564"
FT ACT_SITE 50
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 255
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 49
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 189
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 290
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 69..72
FT /evidence="ECO:0000250"
SQ SEQUENCE 299 AA; 33646 MW; 4877F49F6248C5C5 CRC64;
MLHCITTIFL SISRMTMEDG PIAESYFTAY ADVCFREFGN HVKFWTTINE ANVFTIGGYN
DGTSPPGRCS NCSSGNSSTE TYIVGHNLLL AHASVSRLYQ QKYKDKQGGS VGFSLYAFEF
IPQTSSSKDD EIAIQRAKDF FYGWILGPLT FGDYPDEMKR AVGSRLPIFS KEESEQVKGS
SDFIGIMHYF PALVENIKLK PSLSRNTDFY SDMGVSLTYL GNFSGFGYDV FPWAMESVLE
YIKQTYGNPP VYILENGTPM KPDLELQQKD TRRIEYLQAY IGAVLKAVRN GSDTRGYFV