ID   BGL03_ORYSJ             Reviewed;         505 AA.
AC   Q8RZL1; Q0JIB3; Q5N772;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Beta-glucosidase 3;
DE            Short=Os1bglu3;
DE            EC=3.2.1.21;
DE   Flags: Precursor;
GN   Name=BGLU3; OrderedLocusNames=Os01g0813800, LOC_Os01g59840;
GN   ORFNames=OSJNBa0085D07.14-1, P0432B10.18;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA   Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA   Ketudat Cairns J.R.;
RT   "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT   beta-glucosidase.";
RL   BMC Plant Biol. 6:33-33(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC         with release of beta-D-glucose.; EC=3.2.1.21;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB90397.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD82684.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF06515.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP003570; BAB90397.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP004331; BAD82684.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008207; BAF06515.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q8RZL1; -.
DR   SMR; Q8RZL1; -.
DR   STRING; 4530.OS01T0813800-02; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   PaxDb; Q8RZL1; -.
DR   PRIDE; Q8RZL1; -.
DR   eggNOG; KOG0626; Eukaryota.
DR   InParanoid; Q8RZL1; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR   GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..505
FT                   /note="Beta-glucosidase 3"
FT                   /id="PRO_0000390322"
FT   ACT_SITE        189
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        405
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         43
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         450
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         457..458
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        221
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        415
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        436
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        208..217
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   505 AA;  56647 MW;  F3852AC1B79EA12C CRC64;
     MAAAAAFFCA LLFISVQHGV LGGYTRNDFP ADFVFGAATS AYQYEGAAAE DGRGASIWDT
     FTHAGKMKDK STGDVASDGY HKYKGDVKLM TETGLEAYRF SISWSRLIPS GRGAVNQQGL
     KYYNNIIDEL TKRGIQVHVM LYHLDLPQAL EDEYAGWLSP RIVEDFTAYA DVCFREFGDR
     VSHWTILAEP NVAALGGYDT GEFAPGRCSD PFGVTKCTVG NSSVEPYVAA HNMILTHAAV
     VRLYREKYQT LQKGIVGINV LSLWSYPLTD STADLQAAQR YKDFTYGWIL HPLVFGDYPQ
     VMKKAIGSRL PSFSKVQTEL VKGTLDFIGV NHYFSLYVSD LPLAKGVRDF IADRSVSCRG
     LLQGVRFIAQ TMQAPTRSMG DPHGLQLMLQ HLKESYGDLP IYVQENGKYR KASSNDSLDD
     TDRVDYIKGY IEGVLNATRN GVNARGYFAW FFVDMFELLS GYQTRYGLYR VDFDDAALPR
     RAKRSARWYR DFLKSKRQPL QIAQQ